Abstract: Plantlets regeneration from leaf explants of Phalanopsis was studied. The results showed that:(1) Decontamiated-materials could be obtained by inducing from floral stalk, and proliferation of plantlet was achieved on Murashige and Skoog(MS) medium supplemented with 6-BA(7. 5 mg/L)+NAA(1.0 mg/L); (2)6-BA and NAA can stimulate PLBs(protocorm-like bodies) formation and the effective ruslts could be obtained on MS 6-BA(10.0 mg/L)+NAA(1.0 mg/L); (3)The optimal mediums were 1/2MS and Hyponex mediums for proliferation and transformation of PLBs; (4)The relations between the concentration of sucrose and plantlets regenerations from PLBs could be formulated by y=-0. 019 92 x2+0. 077 51 x+0. 170 00, and plantlets developed well on 1/2 MS containing 20 g/L without any growth regulators.