南京林业大学学报(自然科学版) ›› 2006, Vol. 30 ›› Issue (01): 72-74.doi: 10.3969/j.jssn.1000-2006.2006.01.018

• 研究论文 • 上一篇    下一篇

免疫亲和柱净化法对奶酪中黄曲霉毒素的检测

曾义1,叶建仁2   

  1. 1. 淮安出入境检验检疫局, 江苏 淮安 223001; 2. 南京林业大学森林资源与环境学院, 江苏 南京 210037
  • 出版日期:2016-03-28 发布日期:2016-03-28

Immunoaffinity Column Clean-up of Five Aflatoxins from Cheese and Analysis by HPLC

ZENG Yi1, YE Jian-ren2   

  1. 1. Huaian Entry-exit Commodity Inspection and Quarantine Bureau, Huaian 223001, China; 2. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China
  • Online:2016-03-28 Published:2016-03-28

摘要: <正>研究了江苏南%--和浙江舟山地区松材线虫病木标本,并进行分离培养,得到10多种真菌,其中Ophiostmaips。Ophiostomalignicola,Sphaeropsissalicicola,Dothiorellagregaria和Haplosporellaminor出现的最多;在Ophioslo~naspp。和Cephalosporiumzonatum的培养皿中出现大量的线虫。在天牛的蛹室和虫道中有大量的长喙壳真菌存在,并与线虫的关系密切。实验证明Sphaeropsissalicicola,Dothiorellagregaria、Haplosporellaminor能引起严重的松木变色。

Abstract: A method based on immunoaffinity column clean-up and reversed-phase highperformance liquid chromatography (RP-HPLC) with fluorescence detection has been applied to determinate aflatoxins B1, B2, G1, G2 and M1 from spiked cheese. Chromatographic conditions were bettered by gradient elution technique. Calibration curves of aflatoxins G1, B1 and M1 were linear up to 2~20μg/L, and the calibration curves of aflatoxins G2 and B2 were linear up to 0.6~6.0μg/L. Limits of determination of B1, B2, G1, G2 and M1 were 0.2, 0.3, 0.06, 0.1 and 0.4μg/L, respectively. Average recoveries of the aflatoxins B1, B2, G1, G2 and M1 from cheese spiked with three different concentration levels were greater than 76%, and the RSDs ranged from 1.7% to 4.5%.

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