南京林业大学学报(自然科学版) ›› 2006, Vol. 30 ›› Issue (01): 79-83.doi: 10.3969/j.jssn.1000-2006.2006.01.020

• 研究论文 • 上一篇    下一篇

大戟科4种植物内生真菌分离与抑菌研究

戴传超1,余伯阳2,赵玉婷1,蒋继宏3,杨启银1   

  1. 1. 南京师范大学生命科学学院微生物工程重点实验室, 江苏 南京 210097; 2. 中国药科大学中药复方研究室, 江苏 南京 210009; 3. 江苏省药用植物生物技术重点实验室, 江苏 徐州 221116
  • 出版日期:2016-03-28 发布日期:2016-03-28

The Screening and Identification of Endophytic Fungi from Four Species of Family Euphorbiaceae and the Strains’ Antibacterial Activity

DAI Chuan-chao1, YU Bo-yang2, ZHAO Yu-ting1, JIANG Ji-hong3, YANG Qi-yin1   

  1. 1. The Key Lab of Microbiology and Bioengineering of Life Science College Nanjing Normal University, Nanjing 210097, China; 2. Department of Complex Recipe of CMM, China Pharmaceutical University, Nanjing 210009, China; 3. Key Lab of Medicinal Plant Biotechnology, Xuzhou 221116, China
  • Online:2016-03-28 Published:2016-03-28

摘要: <正>运用X射线电子探针研究了硅对盐胁迫下金丝小枣(Ziziphu5jujuba:cv. Jinsixiaozao)根系中离子微域分布的影响。结果表明:盐胁迫(NaCl质量分数为0.3%)处理使得枣苗根系表皮、皮层和中柱细胞中Na+、Cl-的X射线峰较高,而K+峰较低;而盐胁迫处理加入0.4g/kg硅后,根系表皮、皮层和中柱细胞中Na+、Cl-的X射线峰显著降低,而K峰则大幅度提高,并且使Na’、Cl-在根系各部分呈均匀分布。表明盐胁迫下给土壤施硅可改变金丝小枣体内养分离子的吸收和离子的微域分布,从而缓解盐分离子对金丝小枣的毒害作用。

Abstract: To develop the endophytic fungi from medicinal plants of family Euphorbiaceae, the endophytic fungi from four kinds of medicinal plants of family Euphorbiaceae were screened and identified. The antibacterial activity of these strains was detected. The results showed that 13 strains of endophytic fungi were screened from Sapium sebiferum and 12 of them were identified. These strains belong to Rhizoctonia spp., Sclerotium spp., Pestalotiopsis spp. Acremoniell spp, Chaetomium spp., Coniothyrium spp., Coryneum spp., alternaria spp. 9 strains of endophytic fungi were screened from Euphorbia pekinensis and 8 of them were identified. They were identified as Fusariium spp. and Alternaria spp. 6 strains of endophytie fungi were screened from E. helioscopia and 4 of them were identified. They were identified as Alternaria spp. 15 strains of endophytic fungi were screened from Bischofia polycarpam and 4 of them were identified. They were identified as Dothiorella spp., Coniothyium sp. Phomopsis spp. There are endophytic fungi at the stems. But we can’t find them at leaves of Bischofia polycarpam and four kinds of seeds. 11 strains of 43 (25.58%) showed the steadiness antibacterial activity, 12 strains of 43 (27.91%) strains showed unsteadiness antibacterial activity. The strains showed that steadiness antibacterial activity belonged to Alternaria spp., Fusariium spp., Chaetomium spp., Coniothyium spp. and Phomopsis spp. The results by green grams germinating showed that only one strain was diseased. 21 of total 43 strains were beneficial to the germinating of green grams.

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