Abstract: Betula alnoides is one of the fast growing and high quality timber production tree spices in southern China, but short of genetic improved planting materials supplying. We presented a series of research results of culture mediums difference to induction, multiplication and rooting ability, by seven clones selected from B. alnoides provenances testing plantation. The result indicated that when choosing the second, and the third buds against shoot tip, as explants, have had a highest sprouting rate cultured on B5 medium with the NAA (0.05mg/L). For multiplication, the best medium improved MS1, and for rooting was 1/3MS with IBA(1.0mg/L)+ABT(0.2mg/L)+NAA(0.2mg/L) medium. Among the different clones, clone M16 was the best responding one to the culture medium, however, such a relationship between tissue culture performance and growth still needs to be carefully investigated.