Abstract: Through collecting, isolating and cultivating the pine phyllosphere microbes, several isolates were selected to test their antagonistic effect to Sphaeropsis sapinea and Pestalotiopsis sp. By the method of dual culture, the result showed that the isolates with obviously antagonistic ability to Sphaeropsis sapinea and Pestalotiopsis sp. contained six kinds of bacteria, three kinds of yeast and three kinds of Trichodermas sp., which perhaps could be used for biological control. The antagonistic effect of bacteria is the highest, secondly Trichoderma sp. and yeast lastly. The standard for screening antagonistic fungus is relatively restraining the effect of a fungus. At the same time the restrained proportion and the characteristics leading to disease for the fungus should be considered.