Abstract: The present research was conducted to determine the effects of basal medium, GA3 and sugar on the embryo germination and seedling development of Cyclocarya paliurus by orthogonal design and to induce the obtained shoots to proliferate. The results showed that the basal medium played a determinative role in the embryo germination and seedling development. The best media to promote embryo germination was WPM+GA3 (10mg/L)+sucrose (30g/L), just the E6 treatment according to the orthogonal design, on which the percentage of germination was up to 100%, and the optimal media to develop into seedlings was WPM+GA3 (5mg/L)+maltose (30g/L). Transplanted into the mixture of perlite, peat and vermiculite (1:1:1), the plantlets were all survived after one month. The optimal induction media for proliferation was WPM+6-BA(0.5mg/L), and the mean coefficient of proliferation was 3.45.