Abstract: 22 geographical populations of Anoplohora glabripennis and 1 species of A. chinensis, covering 16 regions in China, 2 regions in USA and 2 regions in South Korea were collected. Those specimens were screened for polymorphic RAPD markers with 17 arbitary primers. There were totally 219 different polymorphic loci obtained, which were sufficient to identify the differences of DNA at the intra-specific and inter-specific levels among these populations studied. According to the relationships of genetic distances in RAPD phylogram, Anoplohora was classified into a clustering branch, geographical populations of A. glabripennis were classied into another clustering branch. In RAPD phylogram, the population of A. glabripennis in USA was classified into an indpendence clustering branch, which showed that the origin of A. glabripennis distributed in USA was not likely from China.