Abstract: The contents of flavonoids and lactones in ginkgo pollen over one-hundred-years-old male ginkgo trees from 15 different regions were analysized by HPLC. The results were as follows: the average content of total flavonoids of ginkgo pollen was 20.44mg/g. The component of lactones firstly detected from ginkgo pollen was ginkolide A, and its average content was 2.22mg/g. Correlation analyses showed that the contents of flavonoids and lactones from ginkgo pollen in different regions didn’t remarkably correlate with climatic and geographical factors. Chongqing-O1, Guangde-O1, Beijing-O1, Kangxian--O1, were pri2 marily selected for selecting superior-individual and establishing pollen-producing plantation of ginkgo.