<正>研究了稀释率、流加糖浓度和初始酵母浓度对树干毕赤酵母单级连续发酵的影响。结果表明：稀释率对单级连续发酵影响较大，当流加糖浓度为45g/L、稀释率为0.04～0.06h-1时发酵效果较好，乙醇得率可达0.370～0.372g/g，产率为0.586～0.754g/L·h。在稀释率为0.06h-1时，较为适宜的流加糖浓度为45g/L，此时乙醇产率为0.785g/L·h。只要稀释率和流加糖浓度确定，初始酵母浓度不会对达到“稳态”的单级连续发酵产生影响。当稀释率一定时，发酵40h后基本可达到“稳态”，此后各参数均稳定在一定范围内，可稳定连续发酵104h。

The mixed ionic liquid 2[BMIM]Cl/[BMIM]2SO4 and 2[BPy]Cl/[BPy]2SO4 which were liquid in room temperature were prepared by using low-cost vitriol to partially exchange chlorine ion of [BMIM]Cl and [BPy]Cl. The synthesis of benzyl acetate from sodium acetate and benzyl chloride was investigated in two kinds of mixed ionic liquids, respectively. After comparing the influence of temperature, reaction time and molar ratio of sodium acetate and benzyl chloride on the yield of benzyl acetate, the best condition was obtained. The yield of benzyl acetate would increase by combined 2[BMIM]Cl/[BMIM]2SO4 with 2[BPy]Cl/[BPy]2SO4 and it would reach the maximum when the molar ratio of 2[BMIM]Cl/[BMIM]2SO4 and 2 [BPy]Cl/[BPy]2SO4 is 1∶1. All these results offered a new approach to predigest the production of ionic liquid, decrease the cost and improve applied worthiness.