拟南芥逆境诱导型启动子rd29A的克隆及活性检测

杨春霞; 陈英; 黄敏仁; 李火根

doi:10.3969/j.jssn.1000-2006.2008.01.002

杨春霞，陈英，黄敏仁，李火根

南京林业大学学报（自然科学版） ›› 2008, Vol. 32 ›› Issue (01) : 10.

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南京林业大学学报（自然科学版） ›› 2008, Vol. 32 ›› Issue (01) : 10. DOI: 10.3969/j.jssn.1000-2006.2008.01.002

研究论文

拟南芥逆境诱导型启动子rd29A的克隆及活性检测

杨春霞，陈英，黄敏仁，李火根

作者信息 +

loning of stress-inducible promoter rd29A from Arabidopsis thaliana
?and its activity detection in transgenic tobacco

YANG Chun-xia, CHEN Ying, HUANG Min-ren, LI Huo-gen

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文章历史 +

摘要

以拟南芥基因组DNA为模板,通过特异PCR扩增,克隆逆境诱导表达启动子rd29A。序列分析表明,该启动子与NCBI上已报道rd29A的启动子有9964%的同源性,其序列含有干旱诱导表达元件DRE和ABA作用元件ABRE等顺式作用元件。用rd29A启动子替换pBI121载体上的35S启动子构建新的载体pBrd-GUS,并以农杆菌介导法将其转入烟草。转基因烟草的GUS组织化学染色及PCR分析结果表明,在低温、干旱及高盐等胁迫诱导下,rd29A启动子可增强GUS基因表达,因此，rd29A启动子可应用于植物抗逆基因工程研究。

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杨春霞，陈英，黄敏仁，李火根. 拟南芥逆境诱导型启动子rd29A的克隆及活性检测[J]. 南京林业大学学报（自然科学版）. 2008, 32(01): 10 https://doi.org/10.3969/j.jssn.1000-2006.2008.01.002

YANG Chun-xia, CHEN Ying, HUANG Min-ren, LI Huo-gen. loning of stress-inducible promoter rd29A from Arabidopsis thaliana
?and its activity detection in transgenic tobacco[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2008, 32(01): 10 https://doi.org/10.3969/j.jssn.1000-2006.2008.01.002

中图分类号： S722

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