无菌松材线虫的获得及培养方法研究

贲爱玲1,2,韩正敏1*,韩旭1,孙平1

南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (03) : 99-102.

PDF(3653077 KB)
PDF(3653077 KB)
南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (03) : 99-102. DOI: 10.3969/j.jssn.1000-2006.2008.03.024
研究论文

无菌松材线虫的获得及培养方法研究

  • 贲爱玲1,2,韩正敏1*,韩旭1,孙平1
作者信息 +

A study of the acquisition of sterilized nematode and growth of the pine wood nematode

  • BEN Ai-ling1,2, HAN Zheng-min1*, HAN Xu1, SUN Ping1
Author information +
文章历史 +

摘要

<正>通过室内模拟试验,研究了保水剂种类及施用浓度对土壤水分蒸发量的影响。结果表明,经过不同类型保水剂处理后,各处理土壤的水分蒸发量均随干旱胁迫时间的延长呈先上升后下降的变化趋势,且经过保水剂处理的土壤的水分蒸发量始终低于对照;各保水剂处理中,kh型保水剂处理后土壤的水分蒸发量始终比其他几种保水剂处理的低。在施用浓度上,保水剂质量分数为0.5%和0.3%两处理对土壤保持水分起较好效果,高浓度的保水剂反而不利于土壤水分的保持。说明施用保水剂能够有效地抑制土壤水分蒸发,且只有在生产中施用合适含量的保水剂才能充分发挥其保水效果。

Abstract

Acquisition of sterilized nematode was the basis of disease mechanism and nematode pathogenic study. Different concentrations of H2O2_ and antibiotics were treated to pine wood nematodes and their eggs. The effective sterilizing process was tested. After an analysis, it was seen that the best way to sterilize the surface of the nematode body was to use higher concentration of antibiotics and lower concentration of H2O2 alternately and repeatedly. The sterilized trematode and the nematode carring bacteria were used in the Botrysls cinerea which grew in the medium of PSA or corn. It was found there were no significant differences in growth and reproduction of the nematode.

引用本文

导出引用
贲爱玲1,2,韩正敏1*,韩旭1,孙平1. 无菌松材线虫的获得及培养方法研究[J]. 南京林业大学学报(自然科学版). 2008, 32(03): 99-102 https://doi.org/10.3969/j.jssn.1000-2006.2008.03.024
BEN Ai-ling1,2, HAN Zheng-min1*, HAN Xu1, SUN Ping1. A study of the acquisition of sterilized nematode and growth of the pine wood nematode[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2008, 32(03): 99-102 https://doi.org/10.3969/j.jssn.1000-2006.2008.03.024
中图分类号: S763.3    Q95   

PDF(3653077 KB)

Accesses

Citation

Detail

段落导航
相关文章

/