Abstract: Antibacterial material of silvercontaining hexagonal mesoporous silica (AgHMS) was synthesized using solgel method, and its structure and nature were characterized by means of XRD, FTIR, UVVis, ESEM, EDS, TGDTA and N2 absorptiondesorption technique. The antibacterial ability of the material was also investigated using turbidimetric method. The results indicated that the AgHMS material was thermostable, the particles of silver were embed and evenly distributed in the framework/nonframework. The crystallinity, mesoporous ordered degree and specific surface area changed then silver particles were added into the material. Both the template agent and demoulding temperature had notable effect on the peak position and strength in IR spectra of Si-O. The results of antibacterial experiments showed that AgHMS had strong inhibition on bacterial. It had an increase order to inhibit Staphylococcus aureus, Gemma bacillus, Escherichia coli and Bacillus subtilis. When the dosage of AgHMS was 0.20 mg/L, the growth of B.subtilis was inhibited. B.subtilis was almost completely killed after 12 hours when the dosage of AgHMS was up to 1.00 mg/L.