研究了微波辅助硫酸、氢氧化钠预处理对玉米秸秆酶解糖得率的影响，并对温度、酸碱添加量、预处理时间、液固比4个因素进行了单因素试验分析。结果表明预处理的最佳条件为：微波-硫酸预处理时，温度190℃，硫酸质量浓度为10 g/L，预处理时间3 min，液固比20(硫酸体积(mL)与玉米秸秆质量(g)之比)条件下，预处理得糖率及酶解得糖率分别为44.6%和30.3%；微波-氢氧化钠预处理时，温度130℃，氢氧化钠质量浓度15 g/L，预处理时间7 min，液固比30(氢氧化钠溶液体积(mL)与玉米秸秆质量(g)之比)条件下，预处理得糖率及酶解得糖率分别为1.5%和80.0%。
Abstract
The effects of microwaveassisted sulfuric acid and sodium hydroxide pretreatment on sugar yields of enzymatic hydrolysis of corn stover were studied. Four factors, i.e., temperature, acid or alkali concentration, pretreatment time and liquid to solid ratio were selected in the single factor analysis method. It was found that with microwaveassisted sulfuric acid pretreatment the optimal pretreatment conditions were temperature 190℃, sulfuric acid concentration 10 g/L, pretreatment time 3 min and liquid to solid ratio 20. Under these conditions the sugar yields were 44.6% in pretreatment and 30.3% in enzymatic hydrolysis. On the other hand, with microwaveassisted sodium hydroxide pretreatment the optimal conditions were temperature 130℃, sodium hydroxide concentration 15 g/L, pretreatment time 7 min and liquid to solid ratio 30. Under these conditions the sugar yields were 1.5% in pretreatment and 80.0% in enzymatic hydrolysis.
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参考文献
[1]余世袁. 林产资源的生物转化与利用[J]. 南京林业大学学报,2000,24(2):1-5.
[2]Mosier N, Wyman C, Dale B, et al. Features of promising technologies for pretreatment of lignocellulosic biomass[J]. Bioresource Technology, 2005, 96: 673-686.
[3]Sun Y, Cheng J. Hydrolysis of lignocellulosic materials for ethanol production[J]. Bioresource Technology, 2002, 83: 1-11.
[4]Grous W R, Converse A O, Grethlein H E. Effect of steam explosion pretreatment on pore size and enzymatic hydrolysis of poplar[J]. Enzyme Microb Technol, 1986,8: 274-280.
[5]Hartree M, Dale B E, Craig W K. Comparison of steam and ammonia pretreatment for enzymatic hydrolysis of cellulose[J]. Appl Microbiol Biotechnol, 1988, 29: 462-468.
[6]Ranmahboob J, Nadim F, Monemi S, et al. Optimizing acidhydrolysis: a critical step for production of ethanol from mixed wood chips[J]. Biomass and Bioenergy, 2002, 22: 401-404.
[7]熊键,叶君,梁文芷,等. 微波对纤维素Ⅰ超分子结构的影响[J]. 华南理工大学学报:自然科学版,2000,28(3):84-89.
[8]Azuma J, Tanaka F, Koshijima T. Enhancement of enzymatic susceptibility of lignocellulosic wastes by microwave irradiation[J]. Ferment Technol, 1884, 62(4): 377-384.
[9]Mandels M. Measurement of saccharifying cellulose[J]. Biotechnol Bioeng Symp, 1976(6): 21-23.
[10]石淑兰,何福望. 制浆造纸分析与检测[M]. 北京:中国轻工业出版社, 2004.
[11]宋向阳,余世袁. 植物纤维原料酶水解的工艺条件[J]. 东北林业大学学报,2000,28(6):116-118.
[12]安宏,王树荣,庄新妍,等. 纤维素稀酸水解的试验研究[J]. 新能源及工艺,2005(2):22-25.
[13]刘家键,陆怡. 预处理对纤维素酶降解影响的研究[J]. 林产化学与工业,1995,15(3):67-71.
基金
收稿日期：2008-06-22修回日期：2009-03-15基金项目：江苏省“青蓝工程”资助项目作者简介：仝明(1984—)，硕士生。*姚春才(通讯作者)，教授，研究方向为化工分离和生物化学加工。Email: ccyao@njfu.com.cn。引文格式：仝明,姚春才. 微波辅助预处理对玉米秸秆酶解的影响[J]. 南京林业大学学报:自然科学版,2009,33(4):91-95.
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