Abstract: Cadmium uptake, accumulation and tolerance potential of three weeping willow (Salix babylonica L.) clones were investigated through hydroponic culture. The results showed that changes of growth parameters in three clones were related to clones and Cd levels in the medium, growth parameters of three clones were not significantly influenced at low Cd concentration (10 μmol/L), Cd concentrations in roots and shoots was increased with elevated Cd levels, high accumulation of Cd was found manly in roots, there was remarkable difference on Cd concentration of shoots among clones. At 10 μmol/L Cd, Cd concentration in shoots of three clones was more than 100 μg/g, translocation factors (TF) of three clones were less than 1, however, ratio of total amount of Cd in shoots to Cd in roots was more than 1. Tolerance indexes (TI) of three clones were related to clones and Cd levels in the medium, TIroot of 10 μmol/L Cd was 0.80—093, and TIshoot of 10 μmol/L was 0.74—0.96. Above results suggested that S.babylonica could tolerate and accumulate Cd at low concentration (10 μmol/L), and was suitable for potential phytoremediation.