通过对上海市区6种林型沿海防护林的土壤养分和三大类群土壤微生物(细菌、真菌、放线菌)数量的动态特征进行研究，结果表明：(1)不同土壤层次三大类群微生物数量变化均较大，土壤表层(0~10 cm)的细菌数量是底层(40~60 cm)的2.33~3.67倍；土壤表层(0~10 cm)的真菌数量是底层(40~60 cm)的2.21~3.75倍；土壤表层(0~10 cm)的放线菌数量是底层(40~60 cm)的1.29~2.26倍；(2)不同林型下土壤三大类群微生物数量的季节变化各有其自身特点，变化趋势不一致；(3)三大类群微生物数量从多到少的顺序为：细菌、真菌、放线菌，细菌在土壤微生物中占有绝对优势；(4)不同林型的表层土壤(0~10 cm)中微生物总量差异较大，年内总量从大到小表现为：杨树、杨树+麻栎、榆树+榉树、水杉+杨树、东方杉、水杉、滩地。
Abstract
The number and distributions of soil microorganisms (bacteria, fungi and actinomycetes) and soil fertility factors of six different forest types were studied. The results obtained were as follows: (1)The quantities of microbe were different at different soil depth. The amounts were much higher within 0-10 cm than that within 40-60 cm (bacteria were 2.33-367 times, fungi were 2.21-3.75 times and actinomycetes were 1.29-2.26 times). (2)The distribution of microorganisms was also different from each other in different forest types at different seasons. (3)Number from largest to smallest in order of bacteria, fungi, actinomycetes. Bacteria were the absolute superiority in soil microorganisms. (4)Total number of microorganisms in 0-10 cm soil layer was much different among every forest type and the total from biggest to smallest were as follows: Populus, Populus+Quercus acutissima, Ulmus pumila+Zelkova schnideriana, Metasequoia glyptostoboides+Populus, Taxodium mucronatum×Crytomeria fortunei, Metasequoia glyptostoboides, Beach.
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参考文献
[1]Smith J L, Paul E A. The significance of soil microbial biomass estimations[J]. Soil Biochemistry, 1990(6): 357-396.
[2]Lin X G, Yin R, Zhang H Y, et al. Changes of soil microbiological properties caused by land use changing from ricewheat rotation to vegetable cultivation[J]. Environmental Geochemistry and Health, 2004, 26(2): 119-128.
[3]林先贵. 土壤微生物的研究进展和发展方向[J]. 土壤,1991,23(4):210-213.
[4]张俊忠,陈秀蓉,杨成德,等. 东祁连山高寒草地土壤微生物三大类群的时空动态特征[J]. 水土保持学报,2008,22(5):167-171.
[5]王素娟,苏和,高丽. 库布齐沙地土壤微生物数量初步研究[J]. 中国草地学报,2008,30(6):89-93.
[6]张星杰,刘景辉,李立军,等. 保护性耕作方式下土壤养分、微生物及酶活性研究[J]. 土壤通报,2009,40(3):542-546.
[7]鲁如坤. 土壤农业化学分析方法[M]. 北京:中国农业科技出版社,2000.
[8]许光辉,郑洪元. 土壤微生物分析方法手册[M]. 北京:农业出版社,1986.
[9]Shamir I, Steinberger Y. Vertical distribution and activity of soil microbial population in a sandy desert ecosystem[J]. Microbial Ecology, 2007, 53: 340-346.
[10]王群,尹飞,郝四平,等. 下层土壤容重对玉米根际土壤微生物数量及微生物量碳、氮的影响[J]. 生态学报,2009,29(6):3096-3104.
[11]Salamanca E F, Raubuch M, Joergensen R G. Microbial reaction of secondary tropical forest soils to the addition of leaf litter[J]. Applied Soil Ecology, 2006, 31: 53-61.
[12]陆雅海,张福锁. 根际微生物研究进展[J]. 土壤,2006,38(2):113-121.
[13]魏媛,张金池,喻理飞. 退化喀斯特植被恢复过程中土壤微生物生物量碳的变化[J]. 南京林业大学学报:自然科学版,2008,32(5):71-75.
[14]王海英,宫渊波,龚伟. 不同林分土壤微生物、酶活性与土壤肥力的关系研究综述[J]. 四川林勘设计,2005(3):9-12.
[15]李国雷,刘勇,李俊清,等. 油松飞播林土壤质量评判及其调控[J]. 南京林业大学学报:自然科学版,2008,32(3):19-24.
[16]Oliver D, Sigrun B, Petra R, et al. Shifis in physiological capabilities of the microbiota during the decomposition of leaf litter ina black alder (Alnus glutinosa (Gaertu.)Ⅰ.) forest[J]. Soil Biology and Biochemistry, 2001, 33(7-8): 921-930.
[17]郑凤英,罗伟雄,李乐,等. 威海市区黑松沿海防护林土壤养分和微生物的研究[J]. 生态环境,2008,17(4):1590-1594.
[18]谢龙莲,陈秋波,王真辉,等. 环境变化对土壤微生物的影响[J]. 热带农业科学,2004,24(3):39-47.
[19]夏北成. 植被对土壤微生物群落结构的影响[J]. 应用生态学报,1998,9(3):296-300.
基金
收稿日期：2009-07-02修回日期：2009-11-08基金项目：上海市科技兴农推广项目(沪农科攻字(2006)第4-10号)；“十一五”国家科技支撑计划(2006BAD03A140301)作者简介：李玥(1981—)，博士生。﹡张金池(通信作者)，教授。Email: nfujczhang@sina.com。引文格式：李玥,张金池,王丽,等. 上海市沿海防护林土壤微生物三大类群变化特征[J]. 南京林业大学学报:自然科学版,2010,34(1):43-47.
