在室内对球孢白僵菌(Beauveria bassiana) 6个自然菌株的营养生长、产孢量、孢子活力、 孢子耐热能力、孢子抗紫外线能力及其对萧氏松茎象成虫的毒力等指标进行了研究。结果表明：菌 株B6和B3对萧氏松茎象成虫的毒力最强，20 d的死亡率和白僵率均达到90 %和80 %。B6菌株生长最 快，14 d直径生长量达24.21 mm，产孢量最高，达12.15×107个/mm2，产孢时间最早，108 h就开始 产孢。B3菌株的孢子萌发率最高，36 h后达到71.07 %，且耐热性较好，抗紫外线能力较强，但其在 PPDA培养基上的产孢量最低。因此，B6菌株是防治萧氏松茎象的优良菌株，具有较大应用价值。
Abstract
To find out a high virulent strain of Beauveria bassiana against Hylobitelus xiaoi Zhang, six strains of B.bassiana were tested for their colony growth, sporogenous ability, spore germination rate, heatresistant, ultravioletresistant and virulence to H.xiaoi Zhang adults. The results showed that the virulent strains to H.xiaoi of B6 and B3 were better than the other ones, their mortality both reached 90 % and cadaver rates reached 80 % after 20 days. The mycelium growth rate of the B6 strain, whose mycelium diameter reached 24.21 mm after 14 days, was the fastest of all. Also the B6 strain was the first one who begun to sporulate (108 hours) and its spore production was the highest, which reached 12.15 ×107 spores per square millimeter. Although the spore germination rate of the B3 strain was the highest which reached 71.07 % in 36 hours and both its heatresistant and ultravioletresistant were the best of all, its spore production on PPDA was the lowest. So the B6 strain may be considered as a potential source of a biocontrol agent of H.xiaoi adult.
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基金
收稿日期：2009-05-12修回日期：2009-08-27基金项目：国家林业局重点课 题资助项目(2001-22);江西省科技厅攻关项目作者简介：王丽娜(1981—),硕士。*温小遂(通信作者 ),教授级高级工程师。Email:jxsfzwxs@yahoo.com.cn。引文格式：王丽娜,唐艳龙,李冬,等. 萧氏 松茎象优良白僵菌菌株的筛选[J]. 南京林业大学学报:自然科学版,2010,34(4):74-78.
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