松萎蔫病是一种非常复杂的病害。据报道，松材线虫(Bursaphelenchus xylophilus)及其携带的细菌诱导了该病，但是对于细菌的的作用尚存争议。笔者筛选出杀细菌剂进行了温室和林间防治松萎蔫病的实验。利用每株3年生赤松(Pinus densiflora)注射3 mg的恶喹酸，松萎蔫病防效达到71 %。利用恶喹酸或阿维菌素及两者混合物对大约20年生赤松的试验表明，3种处理均显示了很高的防效。以上结果证明伴生细菌是诱导松萎蔫病的必要因素，对松萎蔫病可利用杀细菌剂或杀细菌剂与杀线剂混合物进行防控。
Abstract
Pine wilt disease is a very complex disease and has been reported to be caused by pine wood nematode, Bursaphelenchus xylophilus and its accompanying bacteria. However, the role of bacteria in the disease is still debatable. In order to elucidate the hypothesis, we selected one antibacterial agent, oxolinic acid, showing potent antibacterial activity and then evaluated its control efficacy against pine wilt disease in greenhouse and field conditions. Among 6 antibiotics tested, oxolinic acid showed the strongest antibacterial activity against 5 bacteria isolated from three strains of pine wood nematode. In in vivo assay, the agent could effectively suppressed the development of pine wilt disease in 3year old seedlings of Pinus densiflora, it showed 71 % control value when injected 3 mg per seedling. The mixture of oxolinic acid and a nematicidal agent abamectin showed higher disease control efficacy against pine wilt disease than either oxolinic acid or abamectin alone. In addition, oxolinic acid or abamectin alone and their mixture could also controlled pine wilt disease in approximately 20year old pine trees under field conditions. These results strongly indicate that the bacteria accompanying with pine wood nematodes are necessary for the occurrence of pine wilt disease and pine wilt disease could be controlled by antibacterial antibiotic alone and a combination of antibacterial and nematicidal agents.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]Cao Y, Han Z M, Li C D. Studies on wilting toxic substances produced in pines infected by pine wood nematodes[J]. Scientia Silvae Sinicae, 1997, 37: 75-79. [2]Chi SY, Han Z M, He Y Q. Studies on the pathogenicity of 10yearold black pine inoculated with aseptic pine wood nematode[J]. Scientia Silvae Sinicae, 2006, 42: 71-73. [3]Han Z M, Hong Y D, Zhao B G. A study on pathogenicity of bacteria carried by pine wood nematode[J]. J Phytopathol, 2003, 151: 683-689. [4]Kawazu K, Kaneko N. Asepsis of pine wood nematode isolate OKD-3 causes it to lose its pathogenicity[J]. Jpn J Nematol, 1997, 27: 76-80. [5]Kawazu K, Zhang H, Yamashita H, et al. Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phenyl acetic acid production[J]. Biosci Biotech Biochem, 1996, 60: 1413-1415. [6]Tan J J, Feng Z X. Population dynamics of pine wood nematode and its accompanying bacterium in the host[J]. Scientia Silvae Sinicae, 2004, 40: 110-114. [7]Yang B J. Advance in research of pathogenetic mechanism of pine wood nematode[J]. Fort Pest Dis, 2002, 1: 27-31. [8]Zhao B G, Lin F. Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of the genus Pseudomonas[J]. For Pathol, 2005, 35: 339-345. [9]Zhao BG, Lin F. Effects of bacteria associated with pine wood nematode (Bursaphelenchus xylophilus) on development and egg production of the nematode[J]. J Phytopathol, 2007, 155: 26-30. [10]Zhao B G, Wang H L, Han S F. Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China[J]. Nematology, 2003, 5: 899-906.
基金
IUFRO2009国际松萎蔫病研讨会学术论文摘要选登
PDF(137596 KB)
