通过组织分离法，对安徽省9县（市）不同健康状态马尾松树干内真菌种类进行了研究。结果表明，不同健康状态马尾松树干均存在大量真菌，笔者分离到长喙壳等24类可鉴定真菌以及一些待鉴定真菌，其中长喙壳和待鉴定菌Ⅰ为木块变色真菌。对不同健康状态马尾松树干内真菌相对检出率进行比较，死亡马尾松中最高，濒死马尾松次之，健康马尾松最低。健康马尾松树干中不存在变色菌，优势真菌为木霉和镰刀菌，濒死马尾松树干优势真菌为待鉴定菌Ⅰ和长喙壳，死亡马尾松树干优势真菌为木霉和待鉴定菌Ⅰ。
Abstract
Fungi in stem of Pinus massoniana were studied. The results showed that a lot of fungi were isolated and identified. Predominant fungi Ophiostoma sp. and the unidentified fungus isolate Ⅰ were sap stained fungi. The number of fungi species in the died stem of P. massoniana was richer than that in the impending death stem, and more abundant than that in the healthy stem . The population of Ophiostoma sp. and the unidentified fungus isolate Ⅰ in the impending death stem of P. massoniana were more than that in the died stem of P. massoniana. The died stem of P. massoniana had the highest population of Trichoderma spp., the impending death stem had less, and the healthy stem had the least.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1]万方浩，郑小波，郭建英.重要农林外来入侵物种的生物学与控制[M].北京:科学出版社，2005.
[2]Kobayashi T, Sasaki K, Mamiya Y. Fungi associated with Bursaphelenchus lignicolus, the pine wood nematode ：Ⅰ [J]. Jpn For Soc,1974 (56):136-145.
[3]Kobayashi T, Sasaki K, Mamiya Y. Fungi associated with Bursaphelenchus lignicolus, the pine wood nematode：Ⅱ [J]. Jpn For Soc,1975(57):184-193.
[4]Wing field.Transmission of pinewood nematode to cut timber and girdled trees [J].Plant Disease,1983(67):35-37.
[5]Kuroda K.Migration speed of pine wood nematodes and activities of other microbes during the development to pine wilt disease in Pinus thunbergii [J].Journal of Japanese Forestry Society, 1992 (74): 383-389.
[6]Noritoshi Maehara,Kazuyoshi Futai.Factors affecting both the numbers of the pinewood nematode, Bursaphelenchus xylophilus (Nematode: Aphelenchoididae), carried by the Japanese pine sawyer, Monochamus alternatus (Coleoptera: Cerambycidae) and the nematodes life history [J]. Appl Entomol Zoo,1996,31(3):443-452.
[7]Noritoshi Maehara, Kazuyoshi Futi.Factors affecting the number of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) carried by several species of beetles [J].Nematology, 2002,5(4): 653-658.
[8]方中达.植病研究方法[M].北京:中国农业出版社,1998.
[9]戴芳澜.真菌的形态和分类[M].北京:中国科学出版社,1987.
[10]邵力平，沈瑞祥，张素轩.真菌分类学[M].北京:中国林业出版社,1984.
[11]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[12]赵桂华,陈啸寅,吴玉柱,等.松材线虫病木中真菌的研究[J].南京林业大学学报:自然科学版,2006,30(2):79-87.
基金
收稿日期：2010-02-21修回日期：2010-06-19基金项目：国家林业局林业公益性行业科研专项项目（200704026）作者简介：鲁国华(1984—)，硕士。*叶建仁（通信作者），教授。Email: jrye@njfu.edu.cn。
PDF(1215762 KB)
