采用红绒盖牛肝菌、劣味乳菇、紫金蜡蘑、美味牛肝菌和2株彩色豆马勃（分别编号Pt1、Pt2）接种895杨扦插苗，发现菌根真菌对895杨的矿质营养吸收有显著的影响。6种菌根真菌处理的895杨树体内铵态氮和硝态氮含量均有不同程度的增加，提高了杨树根系活力，其中红绒盖牛肝菌、劣味乳菇、美味牛肝菌和Pt 2处理的895杨体内硝酸还原酶含量、硝态氮含量以及氮、磷的吸收均显著高于对照；接种红绒盖牛肝菌、劣味乳菇、美味牛肝菌的895杨根系活力提高较为显著，其中接种美味牛肝菌的895杨，根系活力最强。接种紫金蜡蘑、Pt1处理的杨树对于磷的吸收显著高于对照；各处理下钾元素含量差异不显著；接种菌根真菌后895杨体内微量矿质元素含量都有不同程度的增加，铜、铁、锰、锌元素增加量最高，可见优良的外生菌根真菌可以提高宿主对矿质元素吸收的能力。
Abstract
Inoculating ramets of poplar clone 895 with Xerocomus chrysenteron, Lactarius insulsus, Laccaria aemthystea, Boletus edulis, Pisolithus tinctorius 1 and Pisolithus tinctorius 2 indicated that ectomycorrhizal fungi significantly affected the mineral nutrient absorption of poplar clone 895 cutting seedlings. NH+4N, NO-3N content and root activity of poplar clone 895 treated with 6 kinds of ectomycorrhizal fungi were increased variously. Nitrate reductase, NO-3N content and N, P absorption of poplar clone 895 treated with X. chrysenteron, Lactarius insulsus, B. edulis and P. tinctorius 2 were observably higher than control. Root activity of poplar clone 895 treated with X. chrysenteron, Lactarius insulsus and B. edulis were enhanced. B. edulis was the most effective. P absorption of poplar clone 895 treated with Laccaria aemthystea and P. tinctorius 1 was higher than control. After being treated with 6 kinds of ectomycorrhizal fungi, K concentration of poplar 895 changed slightly, but Cu, Fe, Mn, and Zn concentration were highly increased. The results showed that ectomycorrhizal fungi could enhance trace mineral elements absorption of the host.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
参考文献: [1]马琼，黄建国.菌根及其在植物吸收矿质元素营养中的作用[J].吉林农业科学，2003，28(2):41-43.
[2]金辉，亢志华，陈晖.菌根真菌对铁皮石斛生长和矿质元素的影响[J].福建林学院学报，2007，27(1)：80-83.
[3]郝再彬，苍晶，徐仲.植物生理实验[M].哈尔滨：哈尔滨工业大学出版社，2004.
[4]中国科学院南京土壤研究所.土壤和植物中微量元素分析方法[M]. 北京:科学出版社，1979.
[5]宋微，吴小芹，叶建仁．江苏几种杨树优良外生菌根真菌的筛选[J].南京林业大学学报:自然科学版，2009 33(2):81-84.
[6]ElShourbagy M N ，Malibari A. Effect of PEG stress and mycorrhiza on growth and mineral uptake of barley and soybean[J]. J Agron Crop Sci, 1988,161(5):333-338.
[7]刘晓捷，曾明，杜建斌，等．AMF对葡萄扦插苗矿质营养及生长的影响[J].西南农业大学学报:自然科学版，2006, 28(2)：286-289，318.
[8]Muthukumar T， Udaiyan K. Influence of vesicular rarbuscular mycorrhiza and Rhizobium sp. on growth responses and nutrient status of Tephrosia purpurea Pers[J]. Acta Botanica Indica, 1995, 23(1):75-80.
[9]李晓林，姚青.VA菌根与植物的矿质营养[J].自然科学进展, 2000, 10(6):524-531.
[10]Alkaraki Ghazin, Alraddada. Drought stress and VA mycorrhizal fungi effects on growth and nutrient uptake of two wheat genotypes differing in drought resistance[J].Crop Research (Hisar), 1997, 13(2):245-257.
[11]LangenfeldHeyser R, Gao J，Ducic T, et al．Paxillus involutus mycorrhiza attenuate NaClstress responses in the saltsensitive hybrid poplar Populus×canescens[J].Mycorrhiza, 2007, 17:121-131.
[12]Muhsin T M，Zwiazek J J．Colonization with Hebeloma crustuliniforme increases water conductance and limits shoot sodium up take in white spruce (Picea glauca) seedlings[J]. Plant and Soil, 2002, 238(2):217-225．
基金
收稿日期：2010-04-29修回日期：2010-12-20 基金项目：江苏省重大科技支撑与自主创新专项（BE2008393）；国家林业局林业公益性行业科研专项（201004061）作者简介：宋微(1978—)，博士。*吴小芹（通信作者），教授。Email: xqwu@njfu.com.cn。引文格式：宋微，吴小芹，叶建仁. 6种外生菌根真菌对895杨矿质营养吸收的影响[J]. 南京林业大学学报:自然科学版,2011,35(2):35-38.
PDF(1215840 KB)
