Abstract: Bamboo culm brown rot occurs generally, especially in Nanjing，and influences the growth of bamboo such as Phyllostachys glauca and Ph. viridis f. houzeauana,especially for the former. There was no any reports about Ph. glauca infected the disease previously. By isolation and culture of diseased tissue、artificial inoculation test,morphological observation of isolated strain and amplification with universal primer ITS1/ITS4，finally molecular identification with fragment amplified of 559 bp by ITS analysis，the pathogen of Ph. glauca and Ph. viridis f. houzeauana was identified as Fusarium equiseti (Corda) Sacc.. The research result could provide the reference basis for the effective monitoring and control of the disease.