以UPM常熟纸厂PM2网下白水为试样，采取常规纯培养的方法，获得优势菌株20株，根据细菌的菌落形态、颜色，以及革兰氏染色等常见特征，从中选取4株菌落数量较大的菌株（N1、N2、N4、N16）进行进一步研究。通过16S rDNA序列进行分类研究，确定该细菌的分类地位，并结合个体形态与培养特征、常规生理生化特性进行鉴定，确定各细菌所属种。结果表明：N1、N2为肺炎克雷伯氏菌（Klebsiella pneumoniae），其中N2为肺炎克雷伯氏菌鼻硬结亚种（Klebsiella pneumoniae Rhinoscleroma）；N4为鞘氨醇单胞菌属中的一种Sphingomonas azotifigens；N16为肠杆菌属中的一种Enterobacter radicincitans。鉴于菌株 N1、N2相对N4、N16有较厚的荚膜，由此推断N1、N2可能是造成高速纸机运转过程中产生生物膜污染的主要菌种。
Abstract
White water of the wire pit was sampled from the PM2 in UPM(Changshu) Mill. By the conventional pure culture method, 20 dominant microbial strains were selected. Thereafter, 4 strains of these microorganisms, which were obviously different in shape, color and some other common characteristics, were used to conduct further analyses (N1,N2,N4,N16). The phylogenetic relationship of the 4 strains was determined by sequencing the 16S rDNA. Taxonomy of these strains was also studied through physiological and chemical tests, and morphorlogical observation. The experiments indicated that N1 and N2 were Klebsiella pneumoniae, N2 was Klebsiella pneumoniae Rhinoscleroma, additionally; N4 was Sphingomonas azotifigens; N16 was Enterobacter radicincitans. The thicker capsule of strain N1 and N2 against to strain N4 and N16 implied that N1, N2 were Klebsiella pneumoniae, which likely to be the main disease bacterium that brought about pollution to biofilm of the paper machine.
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基金
收稿日期：2010-06-02修回日期：2010-12-17基金项目：南京林业大学科技创新基金项目（X09-030-5）作者简介：赵丰(1985—)，硕士生。*时留新（通信作者），副教授。Email:shiliuxin@njfu.edu.cn。引文格式：赵丰，焦虎美，时留新. 造纸网下白水中细菌的分离和鉴定［J］. 南京林业大学学报:自然科学版,2011,35(3):115-118.
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