Abstract: Nanomaterials have been widely applied on targeting medical treatments, animal cell imaging or transgenic research. Transformation of molecules into plant cells is more complicated due to its cell wall, an extra barrier to the cell membrane. 〖JP2〗Recently, applications of nanomaterials on plant cell have been focused on cellular imaging and transgenic researches. Herein, we mainly introduced the approaches of the synthesis and functionalization of quantum dots, and their recent applications on plant cell biology. In addition, we also reviewed the category, characteristics and of gene nanocarriers, and their applications on plant transgenic researches including walled cells and protoplast cells. The disadvantages of existing gene nanocarriers were large size and cytotoxicity, which could inhibit their further applications on plant cell biology. Therefore, the design and synthesis of novel nanomaterials for plant cells would be important in future.