为满足大量松材线虫虫株长期保存的需要，对其低温冷冻保存技术进行了研究。试验比较了不同种类及浓度冷冻保护剂对松材线虫在低温冷冻保存时的保护效果,以及冷冻线虫繁殖力与致病力的变化。结果表明：使用15 %甘油和4 %DMSO作为冷冻保护剂，经－80 ℃保存1 d后，松材线虫存活率分别为(39.4±6.6) %和(37.5±21.8) %，且冻存10 d、1个月后均没有显著性下降。与未冷冻的线虫相比，冷冻线虫的繁殖力与致病力均未发生改变。
Abstract
The cryopreservation of Bursaphelenchus xylophilus was investigated. The effects of different cryoprotectants (1 %-5 % DMSO, 5 %-25 % glycerol) on cryopreservation of nematodes and the fertility and infectivity of cryonematodes were evaluated. The results indicated that the survival rate of B. xylophilus was up to (39.4±6.6) % or (375±21.8) % after storage for 1 day at －80 ℃ by using 15 % glycerin or 4 % DMSO respectively as cryoprotectant. And it didnt decline significantly after storage for 10 days or a month. When cryopreserved nematodes were plated onto Botrytis cinerea, they reproduced rapidly. Cryopreserved nematodes were as pathogenic as untreated nematodes to Japanese black pines.
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基金
收稿日期：2011-05-23修回日期：2011-08-30基金项目：国家林业局林业公益性行业科研专项项目（200704026）；江苏省有害生物入侵预防与控制重点实验室开放基金项目（PMIS200802）；江苏高校优势学科建设工程资助项目〖ZK)〗〖JP〗作者简介：朱丽华(1970—)，讲师，博士。*叶建仁（通信作者），教授。Email：jrye@njfu.com.cn。
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