百合种间远缘杂交的不亲和性和杂交后代鉴别,影响着百合杂交育种效率。以东方百合杂交品种‘康斯坦萨’(Lilium var. ‘Constanta’)为母本,我国的特有野生百合岷江百合(Lilium regale Wilson)为父本,采用直接授粉法、切柱法、切柱加柱头液法3种方法在温室栽培条件下进行授粉实验; 子房发育至20 d以后,采集不同处理的子房经过表面灭菌处理,横切为0.3 cm的子房薄片,接种于MS+6-BA(0.01 mg/L)+NAA(0.1 mg/L)的培养基上进行胚拯救培养; 用SRAP分子标记对拯救获得的再生植株进行杂种鉴定。结果表明:3种不同授粉方法中,采用直接授粉法子房生长情况最好,切柱加柱头液法次之,切柱法最差。通过胚拯救培养获得一批杂种后代株系。分子检测结果显示,后代中7个株系均具有典型的父本及母本条带。因此可以认为,‘康斯坦萨’和岷江百合远缘杂交的障碍为受精后障碍,通过子房切片胚拯救培养获得的7个株系均为杂种。
Abstract
In this research,Lilium var. ‘Constanta’(oriental lily hybrid)was chosen as female parent, while the endemic wild lily in China. Lilium regale Wilson was regarded as the male parent. Direct stigma pollination, cut-style pollination, and cut-style pollination with smear stigma fluid were carried out for pollination. Ovaries with different pollination method were collected 20 d after pollination and sterilized, the ovaries were transversely cut into 0.3 cm slices, and were embryo rescue cultured on the MS medium supplemented with 0.01 mg/L 6-BA and 0.1 mg/L NAA. SPAP method was used to identify the strains after embryo rescue culture. The results showed that stigma pollination was the most appropriate pollination method. Through the sliced ovaries rescue culture, seven strains were obtained and all the strains exist typical two parents’ molecular band. In conclusions, the barriers would appear after fertilization in this distant hybridization between Lilium var. ‘Constanta’ and Lilium regale Wilson. And all the seven rescued strains are hybrids.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 吴祝华,施季森,池坚,等.观赏百合资源与育种研究进展[J].南京林业大学学报:自然科学版,2006,30(2):113-118.
[2] 龙雅宜,张金政,张兰年.百合—球根花卉之王[M].北京:金盾出版社,1999.
[3] 彭隆金.百合资源与栽培[M].昆明:云南民族出版社,2002.
[4] 张玉静,王志刚,印东升,等.百合主要病害及防治[J].北方园艺,2007(11):202.
[5] 李晓晨.切花百合的病虫害防治[J].广西植保,2006,19(4):26-28.
[6] 张彩霞,明军,刘春,等.岷江百合天然群体的表型多样性[J].园艺学报,2008,35(8):1183-1188.
[7] 王仙芝,张延龙,牛立新,等.秦巴山区六种野生百合感染3种病毒病鉴定及其田间抗病性初步评价[J].中国农业科学,2008,41(11):3618-3625.
[8] Van Tuyl J M, Dien M P, Creij M G M, et al. Application of in vitro pollination, ovary culture and embryo rescue for overcoming incongruity barriers in interspecific Lilium crosses [J]. Plant Science, 1991,(74)115-126.
[9] Van Tuyl J M. Interspecific hybridization of flower bulbs: A review [J]. Acta Hort, 1997, 430:465-471.
[10] Van Tuyl J M, Dijken A,Chi H S,et al. Breakthroughs in interspecific hybridization of lily [J].Acta Hort, 2000, 508:83-88.
[11] Loffler H J, Meijer H, Straathof T P, et al. Segregation of Fusarium resistance in an interspecific cross between Lilium longiflorum and Lilium dauricum[J].Acta Hort,1996,414:203-208.
[12] Chi H S. The efficiencies of various embryo rescue methods in interspecific crosses of Lilium [J]. Bot Bull Acad Sin,2002,43:139-146.
[13] 刘春,穆鼎,明军,等.百合种间杂交受精前障碍的研究[J].园艺学报,2006,33(3):653-656.
[14] 刘妍,郑思乡,关文灵,等.L×OT和OT×L的新型百合离体授粉杂交研究初报[J].西部林业科学,2009,38(1):105-108.
[15] 陈琼,穆鼎,义鸣放,等.不同授粉方法对克服百合杂交受精前障碍的作用[J].中国农业大学学报,2007,12(4):35-40.
[16] 谈晓林,崔光芬,郑思乡,等.百合不同离体授粉方法的杂交结实研究[J].西南农业学报,2011,24(1):270-274.
[17] 罗建让,张延龙,张林华.克服百合自交及杂交障碍方法的初步研究[J].西北农业学报,2007,16(4):260-263.
[18] 卢江杰,吉永胜彦,方伟,等.3种竹类植物杂种的分子鉴定[J].林业科学,2009,45(3):29-34.
[19] 李怀志,张峻,李翔,等.应用SRAP标记对茄子品种进行遗传多样性分析与指纹图谱构建[J].南京农业大学学报,2011,34(4):18-22.
[20] Budak H, Shearman R C, Parmaksiz I, et al. Molecular characterization of buffalo-grass germplasm using sequence-related amplified polymorphism markers [J]. Theor Appl Genet,2004, 108: 328-334.
[21] Alwala S, Kimbeng C, Veremis J, et al. Linkage mapping and genome analysis in a Saccharum interspecific cross using AFLP, SRAP and TRAP markers [J]. Euphytica, 2008, 164(1): 37-51.
基金
收稿日期:2011-10-28 修回日期:2012-03-01
基金项目:国家林业局“948”项目(2007-4-06); 国家自然科学基金项目(30972407)
第一作者:张睿婧,硕士生。*通信作者:席梦利,副教授。E-mail: ximenglinjfu@126.com。黄寿先,教授。E-mail:huangshouxian@163.com。
引文格式:张睿婧,姜珊,席梦利,等. 百合远缘杂交及其杂种鉴别研究[J]. 南京林业大学学报:自然科学版,2012,36(3):23-27.
PDF(970835 KB)
