橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析

周雪梅; 杨礼富; 王真辉; 邹智; 袁坤

doi:10.3969/j.jssn.1000-2006.2012.05.006

周雪梅，杨礼富，王真辉，邹智，袁坤*

南京林业大学学报（自然科学版） ›› 2012, Vol. 36 ›› Issue (05) : 37-41.

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南京林业大学学报（自然科学版） ›› 2012, Vol. 36 ›› Issue (05) : 37-41. DOI: 10.3969/j.jssn.1000-2006.2012.05.006

研究论文

橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析

周雪梅^1,2，杨礼富¹，王真辉¹，邹智¹，袁坤¹*

作者信息 +

Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis

ZHOU Xuemei^1,2, YANG Lifu¹, WANG Zhenhui¹, ZOU Zhi¹, YUAN Kun¹*

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摘要

橡胶树死皮是影响天然橡胶产量的重要限制因子。通过双向凝胶电泳技术(2-DE)研究了橡胶死皮树与健康树胶乳C-乳清蛋白表达谱的差异。以软件分析后共获得31个差异表达的蛋白点，经基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)分析后，搜索NCBInr数据库，结果有10个蛋白点被成功鉴定，其中6个为未知蛋白，其余4个蛋白分别为chain A,structure of ubiquitinlike protein, Rub1；β1,3glucanase；AIR9 protein和flagellar inner dynein arm heavy chain 11。这些蛋白可能在橡胶树死皮发生过程中扮演重要角色。

Abstract

The tapping panel dryness (TPD) in Hevea brasiliensis is a significant limit factor influencing natural rubber production. In this experiment, the twodimensional gel electrophoresis (2DE) was used to study the differentiation of protein expression profiles of latex Cserum between TPD trees and healthy ones of H. brasiliensis. Thirtyone differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) followed by searching for NCBInr database, ten spots were successfully identified. Six of them were identified as the unknown proteins and the remaining 4 were the chain A, structure of ubiquitinlike protein, Rub1; β1, 3glucanase; AIR9 protein and flagellar inner dynein arm heavy chain 11. These proteins might play important roles in the process of TPD occuring.

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周雪梅，杨礼富，王真辉，邹智，袁坤*. 橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报（自然科学版）. 2012, 36(05): 37-41 https://doi.org/10.3969/j.jssn.1000-2006.2012.05.006

ZHOU Xuemei, YANG Lifu, WANG Zhenhui, ZOU Zhi, YUAN Kun*. Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2012, 36(05): 37-41 https://doi.org/10.3969/j.jssn.1000-2006.2012.05.006

中图分类号： S794.1 Q503

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基金

收稿日期：2011-04-16修回日期：2012-06-19
基金项目：中央级公益性科研院所基本科研业务费专项（YWFZX2008-12，2009-12，2010-9）；海南省重点科技项目（90107）；现代农业产业技术体系建设专项资金（CARS-34-02A）
第一作者：周雪梅，硕士生。*通信作者：袁坤，副研究员。E-mail：yuankun628@126.com
引文格式：周雪梅，杨礼富，王真辉,等. 橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析[J]. 南京林业大学学报:自然科学版,2012,36(5):37-41.