Abstract: Nutrient loading has been widely used as an important cultural technique to regulate seedling quality, which can build higher nutrient reserves in seedlings during nursery culture. To test the influence of nutrient loading on the quality of Larix olgensis, one-year-old seedlings were subjected to a continual supplement of nitrogen in August, September and October(equivalent applications of 240, 360 and 420 kg/hm² N, respectively)after the ordinary fertilization in July. At the time of harvest, seedling height and root collar diameter increased by 35.4%-52.4% and 11.3%-19.0% compared with ordinary fertilization. There were no significant differences in morphology and shoot N concentration among seedlings with different N loadings, while the root N concentration increased significantly with fertilization application, suggesting root may be more sensitive to N loading than shoot. The biomass of seedlings increased significantly compared with ordinary fertilization,but didn’t show any differences among the treatments. On all the temperatures treatments, the values of freeze-induced electrolyte leakage(FIEL)in the N-fertilized seedlings was lower than that of unfertilized, which ranged from 13.57% to 23.12% at temperature above -20 ℃. However, once the temperature declined as low as -30 ℃, FIEL values increased sharply to 23.28%-35.59%, whereas that of the seedlings with 360 and 420 kg/ hm² N treatments was significantly lower, demonstrating that the advantage of N loading practices having a possibility of enhancing the cold resistance of Larix olgensis seedling.