通过改变产酶培养基中的营养成分与比例,采用酶活力测定方法,分析培养基中的碳源种类和浓度、氮源组成、碳氮比(mC:mN)等主要因素对里氏木霉合成β-甘露聚糖酶的影响。结果表明,20 g/L微晶纤维素为碳源、含氮素质量比为1:1的硫酸铵和尿素为氮源、 mC:mN=4的培养基组成,是里氏木霉合成β-甘露聚糖酶的最佳条件。在此条件下β-甘露聚糖酶酶活力在发酵96 h时达到最大,β-甘露聚糖酶酶活力和β-甘露糖苷酶酶活力分别为4.48、0.04 μmol/(min·mL)。
Abstract
In order to improve the synthesis of β-mannanase by Trichoderma reesei,and determinate enzyme activity,fermentation medium of nutritional composition and proportion were investigated,which included carbon sources, carbon source concentration, nitrogen sources and ratio, carbon to nitrogen(mC:mN)ratio of four main factors on the biosynthesis of β-mannanase by Trichoderma reesei.The results showed that 20 g/L microcrystalline cellulose used as the carbon source, ammonium sulfate-urea mixture with the ratio of 1:1 used as the nitrogen source, the mC:mN=4 of culture medium composition were optimal nutrient for β-mannosidase synthesis of Trichoderma reesei.The highest β-mannanase activity and β-mannosidase activity were 4.48 and 0.04 μmol/(min·mL), respectively.The maximal β-mannanase activity was gained after 96 h fermentation.
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基金
收稿日期:2012-05-15 修回日期:2012-10-23
基金项目:国家林业公益性行业科研专项项目(20090417); 江苏高校自然科学重大基础研究项目(11KJA220004); 江苏省科技支撑计划(BE2010732); 江苏高校科技创新团队资助项目
第一作者:王静,博士生。*通信作者:勇强,教授。E-mail: swhx@njfu.com.cn。
引文格式:王静,李鑫,朱均均,等. 培养基组成对里氏木霉合成β-甘露聚糖酶的影响[J]. 南京林业大学学报:自然科学版,2013,37(1):101-104.
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