研究了褐环乳牛肝菌、鸡油菌、彩色豆马勃、土生空团菌、白色豆马勃、松乳菇的接种对马尾松苗木的生长、生理代谢和养分吸收的影响,最终筛选出对马尾松幼苗促生效果较好的菌种。结果表明:(1)接种褐环乳牛肝菌、鸡油菌、彩色豆马勃和土生空团菌均能显著增加苗木的苗高、地径、侧根长、侧根数,促进苗木的生长和生物量的积累,而接种白色豆马勃和松乳菇则对马尾松苗木生长和生物量积累无显著影响,其地径生长还低于对照。(2)外生菌根促进了马尾松苗木体内水分增加,根吸收能力加强,叶绿素含量增加,硝酸还原酶活性和可溶性糖含量增加,降低了脯氨酸、丙二醛含量。(3)外生菌根促进了苗木对磷、钾养分的吸收,对马尾松幼苗氮素营养的吸收效果不显著。与对照比,所有菌根化苗木根中氮、磷、钾分配比例减少,而茎中钾的分配比例增加,叶中磷的分配比例增加。总的来看,褐环乳牛肝菌、鸡油菌、彩色豆马勃和土生空团菌无论对苗木的生长、生理、养分吸收促生效果较好,而白色豆马勃和松乳菇则促生效果较差。
Abstract
The effects of seven ectomycorrhizas including Suillus luteus(L.:Fr.)Gray, Cantharellus cibarius Fr., Pisolithus tinctorius(Pers.), Cenococcum geophilum Fr., P. tinctorius(Pers.)Coder et Couch, Lactarius deliciosus(L.:Fr.)Gray on growth, physiological characteristics and nutrition in Pinus massoniana seedlings were studied in greenhouse. The results showed that ectomycorrhiza could significantly improve the growth of seedling shoot, ground diameter, lateral root and could increase number of lateral root and accumulation of biomass by inoculating with Suillus luteus(L.:Fr.)Gray, Cantharellus cibarius Fr., Pisolithus tinctorius(Pers.)and Cenococcum geophilum Fr. Inoculation with P. tinctorius(Pers.)Coder et Couch and Lactarius deliciosus(L.:Fr.)Gray had no significant influence on growth of seedling height, diameter and biomass compared to control group. The ectomycorrhiza could significantly increase the moisture content of seedling, the content of chlorophyll, activity of nitrate reductase and content of soluble sugar, strengthen absorptive ability of root, reduce the content of proline and MDA. The ectomycorrhiza could significantly improve the absorption of P and K compared to N. The ectomycorrhiza reduced the allocation of N, P and K in root of seedlings but increased the allocation of K in stem and P in leaf compared to control group. Generally, inoculation with S. luteus(L.:Fr.)Gray, C. cibarius Fr., P. tinctorius(Pers.)Coder et Couch, C. geophilum Fr. had preferable effect on growth, physiological metabolism, absorption of N, P and K. And there was a poor effect after inoculation with P. tinctorius(Pers.)Coder et Couch and L. deliciosus(L.:Fr.)Gray.
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基金
收稿日期:2012-02-08 修回日期:2012-07-04
基金项目:国家高技术研究发展计划(2011AA100203); 国家自然科学基金项目(31260183); “十一五”国家科技支撑计划(2006BAD24B0301); 贵州重大专项课题(黔科合重大专项字[2012]6001号); 贵州创新团队建设项目(黔科合人才团队(2011)4003); 贵州大学研究生创新基金(省研农2009002)
第一作者:王艺,博士生。*通信作者:丁贵杰,教授,博导。E-mail: guiji
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