外生菌根对马尾松幼苗生长、生理特征和养分的影响

doi:10.3969/j.issn.1000-2006.2013.02.017

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (02): 97-102.doi: 10.3969/j.issn.1000-2006.2013.02.017

外生菌根对马尾松幼苗生长、生理特征和养分的影响

王艺^1,2,丁贵杰^1*

1.贵州大学造林生态研究所,贵州贵阳 550025;
2.贵州民族大学,贵州贵阳 550025

出版日期:2013-04-18 发布日期:2013-04-18
基金资助:
收稿日期:2012-02-08 修回日期:2012-07-04
基金项目:国家高技术研究发展计划(2011AA100203); 国家自然科学基金项目(31260183); “十一五”国家科技支撑计划(2006BAD24B0301); 贵州重大专项课题(黔科合重大专项字[2012]6001号); 贵州创新团队建设项目(黔科合人才团队(2011)4003); 贵州大学研究生创新基金(省研农2009002)
第一作者:王艺,博士生。*通信作者:丁贵杰,教授,博导。E-mail: guiji

Effects of ectomycorrhizal on growth, physiological characteristics and nutrition in Pinus massoniana seedlings

WANG Yi^1,2, DING Guijie^1*

1.Research Institute of Silviculture and Ecology, Guizhou University, Guiyang 550025,China; 2.Guizhou Minzu University,Guiyang 550025,China

Online:2013-04-18 Published:2013-04-18

摘要/Abstract

摘要： 研究了褐环乳牛肝菌、鸡油菌、彩色豆马勃、土生空团菌、白色豆马勃、松乳菇的接种对马尾松苗木的生长、生理代谢和养分吸收的影响,最终筛选出对马尾松幼苗促生效果较好的菌种。结果表明:(1)接种褐环乳牛肝菌、鸡油菌、彩色豆马勃和土生空团菌均能显著增加苗木的苗高、地径、侧根长、侧根数,促进苗木的生长和生物量的积累,而接种白色豆马勃和松乳菇则对马尾松苗木生长和生物量积累无显著影响,其地径生长还低于对照。(2)外生菌根促进了马尾松苗木体内水分增加,根吸收能力加强,叶绿素含量增加,硝酸还原酶活性和可溶性糖含量增加,降低了脯氨酸、丙二醛含量。(3)外生菌根促进了苗木对磷、钾养分的吸收,对马尾松幼苗氮素营养的吸收效果不显著。与对照比,所有菌根化苗木根中氮、磷、钾分配比例减少,而茎中钾的分配比例增加,叶中磷的分配比例增加。总的来看,褐环乳牛肝菌、鸡油菌、彩色豆马勃和土生空团菌无论对苗木的生长、生理、养分吸收促生效果较好,而白色豆马勃和松乳菇则促生效果较差。

Abstract: The effects of seven ectomycorrhizas including Suillus luteus(L.:Fr.)Gray, Cantharellus cibarius Fr., Pisolithus tinctorius(Pers.), Cenococcum geophilum Fr., P. tinctorius(Pers.)Coder et Couch, Lactarius deliciosus(L.:Fr.)Gray on growth, physiological characteristics and nutrition in Pinus massoniana seedlings were studied in greenhouse. The results showed that ectomycorrhiza could significantly improve the growth of seedling shoot, ground diameter, lateral root and could increase number of lateral root and accumulation of biomass by inoculating with Suillus luteus(L.:Fr.)Gray, Cantharellus cibarius Fr., Pisolithus tinctorius(Pers.)and Cenococcum geophilum Fr. Inoculation with P. tinctorius(Pers.)Coder et Couch and Lactarius deliciosus(L.:Fr.)Gray had no significant influence on growth of seedling height, diameter and biomass compared to control group. The ectomycorrhiza could significantly increase the moisture content of seedling, the content of chlorophyll, activity of nitrate reductase and content of soluble sugar, strengthen absorptive ability of root, reduce the content of proline and MDA. The ectomycorrhiza could significantly improve the absorption of P and K compared to N. The ectomycorrhiza reduced the allocation of N, P and K in root of seedlings but increased the allocation of K in stem and P in leaf compared to control group. Generally, inoculation with S. luteus(L.:Fr.)Gray, C. cibarius Fr., P. tinctorius(Pers.)Coder et Couch, C. geophilum Fr. had preferable effect on growth, physiological metabolism, absorption of N, P and K. And there was a poor effect after inoculation with P. tinctorius(Pers.)Coder et Couch and L. deliciosus(L.:Fr.)Gray.

中图分类号:

王艺,丁贵杰. 外生菌根对马尾松幼苗生长、生理特征和养分的影响[J]. 南京林业大学学报（自然科学版）, 2013, 37(02): 97-102.

WANG Yi, DING Guijie. Effects of ectomycorrhizal on growth, physiological characteristics and nutrition in Pinus massoniana seedlings[J].Journal of Nanjing Forestry University (Natural Science Edition), 2013, 37(02): 97-102.DOI: 10.3969/j.issn.1000-2006.2013.02.017.

参考文献

[1] 马琼,黄建国,蒋剑波.接种外生菌根真菌对马尾松幼苗生长的影响[J].福建林业科技,2005,32(2):85-88. Ma Q,Huang J G, Jiang J B. Effect of inoculating with the ectotrophic mycorrhizal epiphyte on the Pinus massoniana seedling growth[J].Journal of Fujian Forestry Science and Technology, 2005,32(2):85-88.
[2] 毕银丽,丁保建,李晓林. VA菌根对冬小麦养分和水分利用的影响[J].土壤通报,2001,32(3):99-101. Bi Y L, Ding B J, Li X L. Acquisition of nutrient and water by VA mycorrhiza associated to winter wheat[J]. Chinese Journal of Soil Science,2001,32(3):99-101.
[3] Hamel C. Prospects and problems pertaining to the management of Arbuscular mycorrhizae in agriculture[J]. Agriculture,Ecosystems and Environment,1996,60(2-3):197-210.
[4] Dodd J C, Dougall T A, Clapp J P,et al. The role of Arbuscular mycorrhizal fungi in plant community establishment at samphire Hoe, Kent, UK-the reclamation platform created during the building of the channel tunnel between France and the UK[J]. Biodiversity and Conservation, 2002,11(1):39-58.
[5] Abu-Zeyad R, Khan A G, Khoo C. Occurrence of Arbuscular mycorrhiza in Castanospermum australe A. Cunn. & C. Fraser and effects on growth and production of castanospermine[J]. Mycorrhiza,1999,9(2):111-117.
[6] Ahiabor B D, Hirata H. Characteristic responses of three tropical legumes to the inoculation of two species of VAM fungi in Andosol soils with different fertilizers[J]. Mycorrhiza,1994,5(1):63-70.
[7] 辜夕容,梁国仕,杨水平,等. 接种双色蜡蘑对马尾松幼苗生长、养分和抗铝性的影响[J]. 林业科学,2005,41(4):199-203. Gu X R,Liang G S,Yang S P, et al. Influences of Laccaria bicoloron the growth,nutrient uptake and aluminum resistance of Pinus massoniana seedlings[J]. Scientia Silvae Sinicae, 2005,41(4):199-203
[8] 宋瑞清,李喜梅,祁金玉. 外生菌根菌不同接种方法对樟子松苗木生长的影响[J].植物生态学报,2008,32(6):1378-1385. Song R Q, Li X M, Qi J Y. Effects of inoculation of ectomycorrhizal fungi on the seedling growth of mongol scotch pine(Pinus sylvestris var.mongolica)[J].Journal of Plant Ecology:Chinese Version,2008,32(6):1378-1385.
[9] 高悦,吴小芹,孙民琴. 马尾松不同菌根苗对氮磷钾的吸收利用[J].南京林业大学学报:自然科学版,2009,33(4):77-80. Gao Y, Wu X Q, Sun M Q. Effects of ectomycorrhizal seedlings of Masson pine on absorption and utilization of N, P and K[J]. Journal of Nanjing Forestry University:Natural Sciences Edition,2009,33(4):77-80.
[10] 于萌,崔明,申鸿,等. 外生菌根真菌对油松幼苗生长的影响[J].四川林业科技,2011,32(2):45-48. Yu M,Cui M, Shen H, et al. Effect of Inoculating with the Ectotrophic mycorrhizal fungi on the growth of Pinus tabulaeformis seedlings[J].Journal of Sichuan Forestry Science and Technology,2011,32(2):45-48.
[11] 周德庆.微生物学教程[M]. 北京:高等教育出版社,1993.
[12] 张志良,沈曾佑,沈宗英,等.植物生理学实验指导[M].北京:高等教育出版社,1990.
[13] 郝建军,康宗利,于洋,等.植物生理学实验技术[M].北京:化学工业出版社,2007.
[14] Maruland A A, Azc N R, Ruia-Lonazo J M. Contribution of six Arbucular mycorrhizal fungal isolates to water uptake by Lactuna sativaplants under drought stress[J].Physiol Plant,2003,119(4):526-533.
[15] Roden J S, Ball M C. The effect of elevated CO₂ on growth and photosynthesis of two Eucalyptus species exposed to hight temperatures and water defieits [J]. Plant Physiol,1996,11l(3):909-919.
[16] Lareher W. Physiological Plant Ecology[M]. Third edition.Berlin:Springer-Verglag, 1995.
[17] 孙宗玖,李培英,阿不来提,等.干旱复水后4份偃麦草渗透调节物质的响应[J].草业学报,2009,18(5):52-57. Sun Z J,Li P Y,Abulaiti,et al. Response of osmotic adjustment indexes of four Elytrigia repensunder re-watering after drought stress[J].Acta Prataculturae Sinica,2009,18(5):52-57.
[18] 赵祥,侯志兵,董宽虎,等.分胁迫及复水对达乌里胡枝子酶促防御系统影响[J].草业学报,2010,18(2):199-211. Zhao X,Hou Z B,Dong K H,et al. Effects of drought stress and rewatering on enzymatic defensive system in Lespedeza davurica(Laxm.)Schindl[J]. Acta Agrestia Sinica, 2010,18(2):199-211.
[19] 刘娟,董宽虎.干旱胁迫及复水处理对白羊草抗旱生理特性的影响[J].草原与草坪,2011,31(2):74-78. Liu J,Dong K H. Study on the physiological characteristics of Othriochloa ischaemumunder drought stress and re-watering treatments[J]. Grassland And Turf,2011,31(2):74-78.
[20] Porcel R, Ruiz-Lozano J M. Arbuseula rmycorrhizal influence on leaf water Potential,solute accumulation,and oxidative stress in soybean plants subjected to drought stress[J]. J Exp Bot,2004,55(403):1743-1750.
[21] Rabie G H,Almadini A M. Role of bioinoculants in development of salt-tolerance of Vicia faba plants under salinity stress[J]. Afr J Biotechnol,2005,4(3):210-222.
[22] 杨艳. 外生菌根真菌提高油松抗旱性的研究[D].杨凌:西北农林科技大学,2010. Yang Y. Mechanism of the promotion of drought resistance of Pinus tabulaeformis Carr. with ECMF[D].Yangling: Northwest Agriculture and Forestry University,2010.
[23] 王艺,丁贵杰. 干旱胁迫下外生菌根真菌对马尾松幼苗生长[J]. 浙江农林大学学报, 2012, 29(6): 822-828. Wang Y, Ding G J. Growth and microelement absorption for Pinus massoniana seedlings with ectomycorrhizal and water stress[J]. Journal of Zhejiang A & F University, 2012, 29(6): 822-828.
[24] 张小龙,张洪,张香,等. 外生菌根菌剂对白皮松幼苗生长效应的研究[J].林业科学研究, 2005,18(2):133-136. Zhang X L,Zhang H,Zhang X. Study on the effects of ectomycorrhizal preparation on seedling growth of Pinus bungeana[J]. Forest Research, 2005,18(2):133-136.
[25] 张晓兵,李喜梅,向儒江,等. 外生菌根菌对红松苗木生长的影响[J].林业科技,2009,34(3):30-32. Zhang X B, Li X M, Xiang R J, et al. Effect of ectomycorrhizal fungi on the growth of Pinus koraiensis[J]. Forestry Science & Technology, 2009,34(3):30-34.
[26] 付瑞,郭素娟,马履一. 菌根化栓皮栎苗木对不同土壤水分条件的形态和生理响应[J]. 西北林学院学报, 2011, 26(2): 101-104. Fu R, Guo S J, Ma L Y. The Morphology and physiological response of mycorrhizal seedlings of Quercus variabilis under different soil moisture levels[J]. Journal of Northwest Forestry University,2011, 26(2): 101-104.
[27] Mosse B. Growth and chemical composition of mycorrhizal and non-mycorrhizal apples[J].Nature,1957,179:992-994.
[28] 陈应龙,弓明钦,王凤珍,等.混合接种Glomus与Pisolithus菌株对尾叶桉矿质营养吸收的影响[J].林业科学研究,1999,12(3):262-267. Chen Y L,Gong M Q,Wang F Z,et al. Effect on nutrient acquisition of Eucalyptus urophylla inoculated with Glomus and/or Pisolithus[J]. Forest Research,1999,12(3):262-267.
[29] 周志春,陈连庆,黄秀凤.马褂木菌根真菌筛选和菌根化育苗效果研究[J].林业科学研究,2009,22(2):196-199. Zhou Z C,Chen L Q,Huang X F. Screening of chinese tulip tree mycorrhizal fungi and effect of growing seedling with mycorrhizae[J]. Forest Research,2009,22(2):196-199.
[30] John D M, David A P. Basal and maintenance respiration of mycorrhizal and nonmycorrhizal root systems of conifers[J]. Canadian Journal of Forest Research,1987,17(8): 872-877.
[31] Wallander H, Arnebrant K, Dahlberg A. Relationships between fungal uptake of ammonium, fungal growth and nitrogen availability in ectomycorrhizal Pinus sylvestris seedlings[J].Mycorrhiza,1999,8(4): 215-223.
[32] Wallander H. Uptake of P from apatite by Pinus sylvestris seedlings colonized by different ectomycorrhizal fungi[J]. Plant and Soil,2000,218(1-2): 249-256.
[33] Pera J, A’lvarez I F, Rincón A,et al. Field performance in northern Spain of Douglas-fir seedlings inoculated with ectomycorrhizal fungi[J]. Mycorrhiza,1999,9(2): 77-84.
[34] Rennenberg H. The significance of ectomycorrhizal fungi for sulfur nutrition of trees[J]. Plant and Soil,1999,215(2): 115-122.

外生菌根对马尾松幼苗生长、生理特征和养分的影响

Effects of ectomycorrhizal on growth, physiological characteristics and nutrition in Pinus massoniana seedlings

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