采用5个盐分梯度,对10种墨西哥柏( Cupressus lusitanica )幼苗进行盐胁迫处理。通过对苗高、地径净生长量、叶片含水量、叶绿素总含量、相对电导率、脯氨酸和丙二醛含量等指标的测定,观测其在盐胁迫下的一系列生理生化反应。结果表明:随着盐胁迫的加剧,墨西哥柏苗高、地径净生长量、叶片含水量逐渐降低,且盐胁迫对苗高的影响较对地径的大; 叶片脯氨酸、丙二醛含量则随着盐胁迫程度增加显著上升; 叶绿素总含量在低强度胁迫下增大而在高强度胁迫下降低; 相对电导率不能真实地反映出墨西哥柏受盐胁迫的影响程度。根据隶属函数法综合评价各种源耐盐能力,筛选出了3个强抗盐种源为: D10(‘Ejido sierra de agua’)、D3(‘La cienega’)、D4(‘La cumbre del manzano’)。
Abstract
This article aimed to determine the effect of salt stress on physiological and biochemical index of 10 provenances of Cupressus lusitanica by measuring seedling height, ground diameter, relative water content, chlorophyll content, relative conductivity, proline content and malonaldehyde(MDA)content under five salinity gradient levels. The results showed that under salt stress, the seedling’s height, ground diameter and relative water content decreased, while proline content and malonaldehyde(MDA)content increased outstandingly. Salt stress affected the height growth more than ground diameter growth. Total content of chlorophyll increased under low level of salt stress and decreased under high level of salt stress. Relative conductivity could not show the real growth condition of Cupressus lusitanica suffering salt stress. Based on subordination function method, the three high salt-resistant provenances(D10,D3 and D4)among ten provenances are obtained.
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基金
收稿日期:2012-03-15 修回日期:2013-05-15
基金项目:国家林业局“948”项目(2009-4-17)
第一作者:刘奕琳,博士生。*通信作者:万福绪,教授。E-mail:fxwan@njfu.edu.cn。
引文格式:刘奕琳,万福绪,娄晓瑞. 盐胁迫对10个墨西哥柏种源幼苗生理生化的影响[J]. 南京林业大学学报:自然科学版,2013,37(4):29-33.
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