杨树MAGPIE和JACKDAW基因克隆、表达及蛋白互作研究

doi:10.3969/j.issn.1000-2006.2014.03.006

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (03): 29-34.doi: 10.3969/j.issn.1000-2006.2014.03.006

杨树MAGPIE和JACKDAW基因克隆、表达及蛋白互作研究

宣磊,胥猛,徐立安,黄敏仁^*

南京林业大学森林资源与环境学院,江苏南京 210037

出版日期:2014-05-15 发布日期:2014-05-15
基金资助:
收稿日期:2014-01-15 修回日期:2014-03-06
基金项目:国家自然科学基金项目(31100484); 江苏省高校自然科学研究重大项目(13KJA220002); 国家高技术研究发展计划(2013AA102703); 江苏省普通高校研究生科研创新计划(CXZZ13_0549)
第一作者:宣磊,博士生。*通信作者:黄敏仁,教授。E-mail:mrhuang@njfu.edu.cn。
引文格式:宣磊,胥猛,徐立安,等. 杨树MAGPIE和JACKDAW基因克隆、表达及蛋白互作研究[J]. 南京林业大学学报:自然科学版,2014,38(3):29-34.

Cloning, expression and interaction analysis of MAGPIE and JACKDAW genes in Populus

XUAN Lei, XU Meng, XU Li’an, HUANG Minren^*

College of Forest Resource and Environment,Nanjing Forestry University,Nanjing 210037,China

Online:2014-05-15 Published:2014-05-15

摘要/Abstract

摘要： 利用RACE技术从杨树不定根中克隆获得PeMGP和PeJDK基因全长cDNA序列,分别包含1 434 bp和1 518 bp长度的开放阅读框。两者都含2个内含子,其氨基酸序列均有4个保守结构域:2个C₂H₂型锌指结构域ZF1(CX_2-4CX₃FX₄LX₂HX_3-5H)和ZF2(CX₄CX_nHX_nX₄H),2个C₂HC型锌指结构域ZF3(CX₂CX_nHX₃C)和ZF4(CXCX_nHX₃C)。在根茎叶中均检测到PeMGP和PeJKD基因的表达,但它们在杨树不定根发育过程中具有动态的表达模式。杨树原生质体瞬时表达揭示,PeMGP和PeJDK蛋白都定位于细胞核,两者在细胞核发生相互作用。

Abstract: The PeMGP and PeJDK genes were cloned from adventitious roots of Populus, and their full-length cDNA sequences contain open reading frame(ORF)of 1 434 bp and 1 518 bp, respectively. There were two introns in their genomic structure. The two deduced proteins all contained 4 identical zinc finger domains, ZF1(CX_2-4CX₃FX₄LX₂HX_3-5H)and ZF2(CX₄CX_nHX_nX₄H), ZF3(CX₂CX_nHX₃C)and ZF4(CXCX_nHX₃C). Quantitative real-time PCR analysis revealed that two genes expressed in different organs, and showed a dynamic expression pattern in Populus adventitious roots. Transient gene expression assay in Populus mesophyll protoplasts demonstrated that PeMGP and PeJDK were localized in nucleus, and their protein interact each other in nucleus.

中图分类号:

Q781
S722

宣磊,胥猛,徐立安,等. 杨树MAGPIE和JACKDAW基因克隆、表达及蛋白互作研究[J]. 南京林业大学学报（自然科学版）, 2014, 38(03): 29-34.

XUAN Lei, XU Meng, XU Li’an, HUANG Minren. Cloning, expression and interaction analysis of MAGPIE and JACKDAW genes in Populus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(03): 29-34.DOI: 10.3969/j.issn.1000-2006.2014.03.006.

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杨树MAGPIE和JACKDAW基因克隆、表达及蛋白互作研究

Cloning, expression and interaction analysis of MAGPIE and JACKDAW genes in Populus

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