桂花品种SSR荧光指纹图谱的构建

doi:10.3969/j.issn.1000-2006.2014.S1.001

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (增刊): 1-6.doi: 10.3969/j.issn.1000-2006.2014.S1.001

• 研究论文 • 下一篇

桂花品种SSR荧光指纹图谱的构建

段一凡,王贤荣^*,梁丽丽,李雪霞,向其柏

南京林业大学木犀属新品种国际登录中心,江苏南京 210037

出版日期:1900-01-01 发布日期:1900-01-01
基金资助:
收稿日期:2014-09-10
基金项目:江苏省博士创新基金项目; 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:顾一凡,博士。*通信作者:王贤荣,教授,博士。E-mail:wangxianrong66@njfu.edu.cn。
引文格式:段一凡,王贤荣,梁丽丽,等. 桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报:自然科学版,2014,38(S1):1-6.

Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

DUAN Yifan, WANG Xianrong^*, LIANG Lili, LI Xuexia, XIANG Qibai

International Cultivar Registration Center for Osmanthus, Nanjing Forestry University, Nanjing 210037, China

Online:1900-01-01 Published:1900-01-01

摘要/Abstract

摘要： 利用SSR荧光标记技术筛选出的11对SSR荧光标记引物,构建了64个桂花品种的SSR指纹图谱。11对SSR引物共扩增出143个等位基因,平均每个位点13个等位基因。群体平均的观测杂合度(H_o)、期望杂合度(H_e)、Shannon’s信息指数(I)、观察等位基因数(N_a)和有效等位基因数(N_e)分别为0.619 7、0.848 3、2.323 7、17.833 3和8.228 2。四季桂与秋桂(金桂、银桂和丹桂)的遗传距离较远。利用引物OF002、OF019、OSM63和OSM63中的任意2对可区分所有供试品种。64个桂花品种的SSR指纹图谱互不相同,可以作为各品种特定的图谱,为品种鉴别提供依据。

Abstract: In this study, fluorescence-labeled simple sequence repeat(SSR)markers were used to fingerprinting the 64 Osmanthus fragrans cultivars under cultivation in China. Eleven pairs of SSR primers screened amplified 143 alleles,with an average of 13 alleles per locus. The average of H_o, H_e, I, N_a and N_e was0.619 7, 0.848 3, 2.323 7, 17.833 3 and 8.228 2, respectively. The Asiaticus Group had a distance relationship with the remaining groups. Two of OF002, OF019, OSM63 and OSM63 could distinguish all of the O. fragrans cultivars. Sixty-four O. fragrans cultivars with different SSR fingerprints of each other could serve as the cultivars-specific patterns and as an important basis for cultivars identification.

中图分类号:

Q948
S685.13

段一凡,王贤荣,梁丽丽,等. 桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报（自然科学版）, 2014, 38(增刊): 1-6.

DUAN Yifan, WANG Xianrong, LIANG Lili, LI Xuexia, XIANG Qibai. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(增刊): 1-6.DOI: 10.3969/j.issn.1000-2006.2014.S1.001.

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桂花品种SSR荧光指纹图谱的构建

Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers

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