抗松材线虫病赤松胚性愈伤组织的诱导及增殖

吴静,朱丽华,许建秀,吴小芹,叶建仁

南京林业大学学报(自然科学版) ›› 2015, Vol. 39 ›› Issue (01) : 17-21.

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南京林业大学学报(自然科学版) ›› 2015, Vol. 39 ›› Issue (01) : 17-21. DOI: 10.3969/j.issn.1000-2006.2015.01.004
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抗松材线虫病赤松胚性愈伤组织的诱导及增殖

  • 吴 静,朱丽华,许建秀,吴小芹,叶建仁*
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Induction and proliferation of embryogenic callus in nematode-resistant Pinus densiflora

  • WU Jing, ZHU Lihua,XU Jianxiu, WU Xiaoqin, YE Jianren*
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摘要

以抗松材线虫病赤松未成熟合子胚为外植体,研究了合子胚发育阶段、激素种类及浓度以及碳源等对胚性愈伤组织诱导的影响。结果表明:发育至2~4阶段的合子胚为胚性愈伤组织诱导的适宜外植体,2,4-D和6-BA是最优激素组合,麦芽糖比蔗糖、葡萄糖更有利于胚性愈伤组织的诱导。抗松材线虫病赤松胚性愈伤组织诱导率总体较低,以DCR为基本培养基,添加2 mg /L 2,4-D和1 mg /L 6-BA时诱导率最高为5.0%,诱导的胚性愈伤组织在增殖培养基上能够稳定增殖。

Abstract

Embryogenic callus was induced from immature embryos of nematode-resistant Pinus densiflora. Factors affecting callus initiation were investigated. The results showed that successful initiation of embryogenic callus depended heavily on embryo developmental stage, the combination of auxin and cytokinins, and carbon source. The initiation of embryogenic callus worked best when immature mega gametophytes developed at stage 2-4 were used as starting material. The optimal combination of hormone was 2 mg /L 2,4-D and 1 mg /L 6-BA with the induction rate of 5%. Maltose was better than sucrose and glucose. Further proliferation of embryogenic callus was achieved by transfering explants to a medium with reduced levels of plant growth regulators.

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吴静,朱丽华,许建秀,吴小芹,叶建仁. 抗松材线虫病赤松胚性愈伤组织的诱导及增殖[J]. 南京林业大学学报(自然科学版). 2015, 39(01): 17-21 https://doi.org/10.3969/j.issn.1000-2006.2015.01.004
WU Jing, ZHU Lihua,XU Jianxiu, WU Xiaoqin, YE Jianren. Induction and proliferation of embryogenic callus in nematode-resistant Pinus densiflora[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(01): 17-21 https://doi.org/10.3969/j.issn.1000-2006.2015.01.004
中图分类号: S763    Q813   

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基金

收稿日期:2014-11-03 修回日期:2014-12-08
基金项目:国家林业局“948”项目(2011-04-69); 江苏省科技支撑计划(BE2014405); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:吴静,硕士。*通信作者:叶建仁,教授。E-mail: jrye@njfu.edu.cn。
引文格式:吴静,朱丽华,许建秀,等. 抗松材线虫病赤松胚性愈伤组织的诱导及增殖[J]. 南京林业大学学报:自然科学版,2015,39(1):17-21.

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