美国山核桃种质资源遗传多样性ISSR分析

李晖; 张瑞; 彭方仁; 李永荣; 翟敏

doi:10.3969/j.issn.1000-2006.2015.04.002

李晖,张瑞,彭方仁,李永荣,翟敏

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 7-12.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 7-12. DOI: 10.3969/j.issn.1000-2006.2015.04.002

专题报道

美国山核桃种质资源遗传多样性ISSR分析

李晖¹,张瑞¹,彭方仁^1*,李永荣²,翟敏²

作者信息 +

Genetic diversity and identification of pecan by ISSR markers

LI Hui¹, ZHANG Rui¹, PENG Fangren^1*, LI Yongrong², ZHAI Min²

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文章历史 +

摘要

以161个美国山核桃品种及株系、1个核桃品种和1个山核桃品种为试材,利用ISSR分子标记技术对其进行遗传多样性分析。结果表明:14条ISSR引物在供试品种中共扩增出56条谱带,其中55条为多态性条带,多态性指数达98%; 供试品种的基因多样性指数为0.366 8,Shannon多态性信息指数为0.542 8; 品种间相似系数范围为0.29～0.94,平均为0.649 6,表明这些美国山核桃种质资源间的亲缘关系较近。供试品种(单株)的遗传背景比较复杂,根据聚类分析可将这些品种(株系)划分为9个类群。

Abstract

In this study, ISSR markers were used to evaluate the genetic diversity of 161 pecan cultivars and individuals, one walnut cultivar and one Carya cathayensis cultivars. A total of 56 bands were yielded by 14 ISSR premiers, and 55 were amplified bands(98% polymorphic). Nei’s gene diversity He and Shannon’s information index I were 0.366 8 and 0.542 8, respectively. The Nei’s genetic similarity coefficients ranged from 0.29 to 0.94, and the average was 0.649 6, which showed close genetic relationships. According to the results of cluster analysis, the 163 accessions could be divided into 9 groups, which showed that the genetic background was very complex.

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李晖,张瑞,彭方仁,李永荣,翟敏. 美国山核桃种质资源遗传多样性ISSR分析[J]. 南京林业大学学报（自然科学版）. 2015, 39(04): 7-12 https://doi.org/10.3969/j.issn.1000-2006.2015.04.002

LI Hui, ZHANG Rui, PENG Fangren, LI Yongrong, ZHAI Min. Genetic diversity and identification of pecan by ISSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(04): 7-12 https://doi.org/10.3969/j.issn.1000-2006.2015.04.002

中图分类号： S722

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基金

收稿日期:2014-04-21 修回日期:2015-02-21基金项目:国家林业公益性行业科研专项项目( 201304711); 江苏高校优势学科建设工程资助项目( PAPD) 第一作者:李晖,硕士。*通信作者:彭方仁,教授。E-mail:frpeng@njfu.edu.cn。引文格式:李晖,张瑞,彭方仁,等. 美国山核桃种质资源遗传多样性ISSR分析[J]. 南京林业大学学报:自然科学版,2015,39(4):7-12.