黄栌枯萎病菌拮抗细菌的分离与鉴定

孙妍; 于地美; 黄华毅; 王永林; 田呈明

doi:10.3969/j.issn.1000-2006.2015.06.004

黄栌枯萎病菌拮抗细菌的分离与鉴定

孙妍,于地美,黄华毅,王永林,田呈明

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 17-23.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 17-23. DOI: 10.3969/j.issn.1000-2006.2015.06.004

研究论文

黄栌枯萎病菌拮抗细菌的分离与鉴定

孙妍,于地美,黄华毅,王永林,田呈明^*

作者信息 +

Isolation and identification of an antagonistic bacterium against smoke tree wilt fungus Verticillium dahliae

SUN Yan, YU Dimei, HUANG Huayi, WANG Yonglin, TIAN Chengming^*

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文章历史 +

摘要

为了获得能有效拮抗黄栌枯萎病的大丽轮枝菌(Verticillium dahliae)和尖孢镰刀菌(Fusarium oxysporum)的生防菌株,采用系列稀释分离法和平板对峙法从盐碱地土壤中筛选拮抗细菌,基于形态特征、生理生化特性和16S rDNA序列分析鉴定菌株,并测定其无菌滤液和挥发性气体的抑菌活性。筛选结果表明,菌株C-2-3-2是一株能有效抑制黄栌枯萎病菌和尖孢镰刀菌的生防菌株,经鉴定其为枯草芽孢杆菌(Bacillus subtilis)。该菌株的无菌滤液对黄栌枯萎病菌和尖孢镰刀菌均具有明显的体外抑菌作用,且能有效抑制黄栌枯萎病菌微菌核的形成。同时,其挥发性气体也能有效抑制黄栌枯萎病菌的生长。另外,研究还发现该菌株能产生铁载体和合成氨,并产生几丁质酶、纤维素酶、蛋白酶和β-1,3-葡聚糖酶。研究结果表明菌株C-2-3-2在黄栌枯萎病生物防治中具有较好的应用潜力和较高的研究价值。

Abstract

In order to isolate and characterize an antagonistic bacterial strain against Verticillium dahliae and Fusarium oxysporum, serial dilution and plate-confrontation methods were used for preliminary screening and selecting antagonistic bacteria from saline-alkali soil. Strain identification was based on morphological and physiological characteristics, and phylogenetic analysis of 16S rDNA sequence. The antagonistic effects of sterile culture filtrates and volatiles of antagonistic bacteria were determined. An antagonistic bacteria, named as C-2-3-2, was isolated and identified as Bacillus subtilis. The results indicated that sterile culture filtrate of the strain exhibited obvious in vitro inhibition against V. dahliae and F. oxysporum, and suppressed the production of microsclerotia of V. dahliae. The volatiles also effectively inhibited the mycelial growth of V. dahliae. In addition, the results indicated that C-2-3-2 could produce ammonia and siderophore, and also could produce cell wall degrading enzymes, such as chitinase, cellulose, protease and β-1,3-glucanase. It indicted that strain C-2-3-2 had high research value and potentials for biological control of Verticillium wilt of Cotinus coggygria.

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孙妍,于地美,黄华毅,王永林,田呈明. 黄栌枯萎病菌拮抗细菌的分离与鉴定[J]. 南京林业大学学报（自然科学版）. 2015, 39(06): 17-23 https://doi.org/10.3969/j.issn.1000-2006.2015.06.004

SUN Yan, YU Dimei, HUANG Huayi, WANG Yonglin, TIAN Chengming. Isolation and identification of an antagonistic bacterium against smoke tree wilt fungus Verticillium dahliae[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(06): 17-23 https://doi.org/10.3969/j.issn.1000-2006.2015.06.004

中图分类号： S763.1

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基金

收稿日期:2014-11-24 修回日期:2015-09-11
基金项目:中央高校基本科研业务费专项资金项目(xs201401)
第一作者:孙妍,本科生。*通信作者:田呈明,教授。E-mail: chengmt@bjfu.edu.cn。
引文格式:孙妍,于地美,黄华毅,等. 黄栌枯萎病菌拮抗细菌的分离与鉴定[J]. 南京林业大学学报:自然科学版,2015,39(6):17-23.