槲皮素作为荧光探针对醋酸根离子的识别作用

徐园园; 杨世龙; 姜维娜; 赵俸艺; 印彬; 徐莉

doi:10.3969/j.issn.1000-2006.2016.01.013

徐园园,杨世龙,姜维娜,赵俸艺,印彬,徐莉1,3,4*, 高步红,孙海军,杜丽婷,唐颖3,5,曹福亮1,5

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (01) : 80-86.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (01) : 80-86. DOI: 10.3969/j.issn.1000-2006.2016.01.013

研究论文

槲皮素作为荧光探针对醋酸根离子的识别作用

徐园园^1,4,杨世龙^1,2,姜维娜^1,2,赵俸艺^1,4,印彬⁴,徐莉^1,3,4*, 高步红³,孙海军³,杜丽婷³,唐颖^3,5,曹福亮^1,5

作者信息 +

A natural quercetin-based fluorescent sensor for sensitive and selective detection of acetate ions

XU Yuanyuan^1,4, YANG Shilong^1,2, JIANG Weina^1,2, ZHAO Fengyi^1,4, YIN Bin⁴, XU Li^1,3,4*, GAO Buhong³, SUN Haijun³, DU Liting³, TANG Ying^3,5, CAO

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摘要

为了探讨天然产物槲皮素作为荧光探针检测醋酸根离子的选择性和灵敏性,实验将不同种类阴离子加入到槲皮素的二甲亚砜溶液中,考查槲皮素溶液的荧光强度变化。实验发现:槲皮素的二甲亚砜溶液在500 nm处有一荧光发射峰,加入醋酸根离子时,该峰强度明显增强,而其他阴离子(Cl^-、Br^-、I^-、ClO^-₄、H₂PO^-₄)对该峰强度无明显影响,同时在Q-Ac^- 中加入其他阴离子未引起荧光强度的变化。表明槲皮素对醋酸根离子具有较好的选择性; 荧光滴定光谱表明槲皮素对醋酸根离子具有较高的灵敏度,且荧光滴定曲线线性关系较好(R²=0.995),线性范围为1.0×10^-6～8.0×10^-6 mol/L,最低检测限为1.0×10^-7 mol/L; 通过Job曲线得到槲皮素与醋酸根离子作用的化学计量比为3:2,稳定常数为3.11×10⁴。核磁等实验表明,醋酸根离子的加入破坏或减弱了体系原有的氢键,促进了槲皮素分子内电荷转移,使槲皮素的荧光强度增强。用该方法检测了样品中微量的醋酸根离子,回收率为98.82%～100.96%,测定结果稳定。

Abstract

A natural quercetin-based fluorescent sensor for sensitive and selective detection of acetate ions has been studied. Changes of fluorescence spectrum were studied before and after the addition of different anions in dimethyl sulfoxide(DMSO)solution of quercetin(Q), fluorescence emission intensity of Q increased obviously, acetate ions enhance fluorescence emission. And the fluorescence emission intensity had no change when adding other anions(Cl^-,Br^-,I^-,ClO^-₄,H₂PO^-₄). All suggested that the progress for detecting acetate ions will not be affected by other anions, and quercetin-based fluorescent sensor has good selectivity. The sensor Q can be applied to the quantification of acetate ions with a linear range of 1.0×10^-6-8.0 × 10^-6 mol/L and the detection limit of 1.0 × 10^-7 mol/L. The stoichiometric ratio of Q and acetate ions was 3:2 obtained by Job's plot. The stability constant is 3.11×10⁴. The ¹H-NMR spectra of Q and Q-Ac^- system showed a probable mechanism of quercetin recognition was that Ac^- could destroy or weaken original hydrogen bonds, and promote charge transfer within quercetin molecule, which resulted in fluorescence intensity increasing. This method had been successfully applied in detecting acetate ions of samples in DMSO with good recovery(98.82%-100.96%)and repeatability.

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徐园园,杨世龙,姜维娜,赵俸艺,印彬,徐莉1,3,4*, 高步红,孙海军,杜丽婷,唐颖3,5,曹福亮1,5. 槲皮素作为荧光探针对醋酸根离子的识别作用[J]. 南京林业大学学报（自然科学版）. 2016, 40(01): 80-86 https://doi.org/10.3969/j.issn.1000-2006.2016.01.013

XU Yuanyuan, YANG Shilong, JIANG Weina, ZHAO Fengyi, YIN Bin, XU Li^1,3,4*, GAO Buhong, SUN Haijun, DU Liting, TANG Ying^3,5, CAO. A natural quercetin-based fluorescent sensor for sensitive and selective detection of acetate ions[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(01): 80-86 https://doi.org/10.3969/j.issn.1000-2006.2016.01.013

中图分类号： O657.3

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基金

收稿日期:2015-06-25 修回日期:2015-10-15
基金项目:国家林业公益性行业科研专项项目(20120460102); “十二五”国家科技支撑计划(2012BAD21B04); 南京林业大学优秀博士学位论文创新基金项目
第一作者:徐园园(yyxu_njfu@163.com)。*通信作者:徐莉(xuliqby@njfu.edu.cn),教授。
引文格式:徐园园,杨世龙,姜维娜,等. 槲皮素作为荧光探针对醋酸根离子的识别作用[J]. 南京林业大学学报(自然科学版),2016,40(1):80-86.