Abstract: In order to establish a high efficient regeneration system of Cinnamomum kanehirae, its stems were used as explants to study the effects of collection season, sterilization methods and different hormone concentrations on stem regeneration in the processes of buds induction. The results were as follows: The lower infecting and browning rate, quicker differentiation speed and higher germination rate were observed when explants were collected in spring and summer. The infectious rate significantly decreased if the explants were treated by thiophanate-methyl solution diluted 1 000 times for 10 min, 75% alcohol for 30 s and 0.1% mercuric chloride for 8-10 min. And these treatments were harmless to explants. MS was an optimal basic medium. The optimum medium for buds induction was MS+1.0 mg/L 6-BA + 0.1 mg/L IBA, which exhibited higher bud induction rate and thicker buds. MS+ 1.5 mg/L 6-BA+ 0.1 mg/L IBA was the optimal medium for bud subculture proliferation, which exhibited higher cluster bud proliferation rate and strong bud. 1/2MS + 0.2 mg/L ABT + 0.05 mg/L IBA +0.5 g/L AC was the optimal medium for root differentiation, with the rooting percentage 100% after 30 days. Establishment of stable plant regeneration system of C. kanehirae will be useful for saving the resources of endangered species and expanding its living space. Also, it will lay a foundation for the improvement of breeding and the genetic transformation system in the future.