Abstract: An experiment was conducted to study the growth responses of Pinus tabulaeformis containerized transplanting through keeping the proportion of perlite powder unchanged, and gradually increasing the proportion of mushroom compost from 0% to 75%, and replacing grass carbon. Eight kinds of media formula were designed in this study. The results showed that it was feasible to use mushroom slag compost as the culture matrix of P. tabulaeformis containerized transplanting. When the proportion of mushroom compost in the mediums reached 40%, the height, diameter, root biomass, shoot biomass and foliar biomass all exhibited no changes if compared with the common peat pressing, while nutrient content was significantly higher than that in the common peat processing. The physical and EC of the treatments were suitable to the growth of P. tabulaeformis .When mushroom compost proportion was ≥50%,the pH value was changed to alkaline from slightly acidic. The elevated matrix pH values may be the main factor leading to decline in the quality of seedlings. Therefore, under this experimental condition, the optimum matrix formula used to develop P. tabulaeformis containerized transplanting is 35% peat, 25% perlite and 40% spent mushroom compost.