香樟齿喙象成虫触角感器的微观特征分析

顾天滋; 张丛丛; 苏鹏; 樊斌琦; 王焱; 郝德君

doi:10.3969/j.issn.1000-2006.201606018

香樟齿喙象成虫触角感器的微观特征分析

顾天滋,张丛丛,苏鹏,樊斌琦,王焱,郝德君

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04) : 89-94.

PDF(2493617 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2493617 KB)

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04) : 89-94. DOI: 10.3969/j.issn.1000-2006.201606018

研究论文

香樟齿喙象成虫触角感器的微观特征分析

顾天滋¹,张丛丛¹,苏鹏²,樊斌琦³,王焱³,郝德君^1*

作者信息 +

Ultrastructure of Pagiophloeus tsushimanus Morimoto antennal sensilla with scanning electron microscope

GU Tianzi¹, ZHANG Congcong¹, SU Peng², FAN Binqi³, WANG Yan³, HAO Dejun^1*

Author information +

文章历史 +

摘要

【目的】香樟齿喙象(Pagiophloeus tsushimanus Morimoto)是新发现的危害香樟(Cinnamomum camphora)的钻蛀性害虫,研究触角感器超微形态有助于了解该害虫的嗅觉行为及其与寄主植物的相互关系。【方法】将成虫触角用超声波清洗并冷冻干燥,采用离子溅射仪喷金镀膜,再利用扫描电镜观察其外部形态、感器类型及分布状况。【结果】香樟齿喙象雌、雄成虫触角均呈膝状,共有7类11种感器类型,分别为3种刺形感器、2种毛形感器、2种锥形感器以及表皮孔、柱形感器、栓锥形感器和叉形感器。刺形感器是香樟齿喙象的主要感器类型,在性别之间除刺形感器Ⅰ型、Ⅱ型长度存在显著差异(P<0.05)外,其他类型感器长度、数量及分布均没有显著差异(P>0.05); 锥形感器和叉形感器仅在雌虫触角上发现。【结论】此次研究观察了香樟齿喙象成虫的触角感器类型,可为开发基于信息素的害虫防治方法提供理论依据。

Abstract

【Objective】Pagiophloeus tsushimanus Morimoto is a wood-boring beetle that has recently been introduced to China causing serious damage to Cinnamomum camphora in Shanghai City and Fujian Province. Investigating the ultrastructure of the pests' antennal sensilla could elucidate its olfactory behaviors and interactions with its host.【Method】The antennae were submerged in an ultrasonic bath, freeze-dried and sputter-coated with gold. Then, the antennae morphology, the type, number and distribution of antennal sensilla were visualized by using scanning electron microscopy.【Result】The adult antennae were geniculate, seven types including 11 subtypes of sensilla were recorded: sensilla chaetica(Sch Ⅰ-Ⅲ), trichoid sensilla(St Ⅰ-Ⅱ), sensilla basiconica(Sb Ⅰ-Ⅱ), cuticular pore, cylindric sensilla, styloconica sensilla and sensilla furcatea. Sensilla chaetica represent the most abundant type of sensillum, and there were no significant sex-specific differences in length, number, or distribution of the antennal sensilla, except for the lengths of Sch Ⅰ and Sch Ⅱ. However, sensilla basiconica and sensilla furcatea were only found in females.【Conclusion】Studying on the ultrastructure of antennal sensilla of female and male P. tsushimanus provides a theoretical foundation for the development of pheromone-based P. tsushimanus control.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

顾天滋,张丛丛,苏鹏,樊斌琦,王焱,郝德君. 香樟齿喙象成虫触角感器的微观特征分析[J]. 南京林业大学学报（自然科学版）. 2017, 41(04): 89-94 https://doi.org/10.3969/j.issn.1000-2006.201606018

GU Tianzi, ZHANG Congcong, SU Peng, FAN Binqi, WANG Yan, HAO Dejun. Ultrastructure of Pagiophloeus tsushimanus Morimoto antennal sensilla with scanning electron microscope[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(04): 89-94 https://doi.org/10.3969/j.issn.1000-2006.201606018

中图分类号： S763

参考文献

[1] ZACHARUK R Y. Ultrastructure and function of insect chemosensilla[J]. Annual Review of Entomology, 1980, 25(1): 27-47. DOI: 10.1093/aesa/saw027.
[2] XI Y Q, YIN X M, ZHU C D, et al. Scanning electron microscopy studies of antennal sensilla of Ooencyrtus phongi (Hymenoptera: Encyrtidae)[J]. Microscopy Research and Technique, 2011, 74(10): 936-945. DOI:10.1002/jemt. 20979.
[3] SUKONTASON K, METHANITIKORN R, CHAIWONG T, et al. Sensilla of the antenna and palp of Hydrotaea chalcogaster (Diptera: Muscidae)[J]. Micron, 2007, 38(3): 218-223. DOI:10.1016/j. micron.2006.07.018.
[4] ONAGBOLA E O, MEYER W I, BOINA D R, et al. Morphological characterization of the antennal sensilla of Asian citrus psyllid, Diaphorina citri Kuwayama(Hemiptera: Psyllidae), with reference to their probable functions[J]. Micron, 2008, 39(8): 1184-1191. DOI:10.1016/j.micron. 2008.05.002.
[5] YAN S C, MENG Z J, PENG L, et al. Antennal sensilla of the pine weevil Pissodes nitidus Roel.(Coleoptera: Curculionidae)[J]. Microscopy Research and Technique, 2011, 74(5): 389-396. DOI:10.1002/jemt. 20920.
[6] 刘鑫海, 骆有庆, 王建伟, 等. 沙蒿大粒象触角感器的扫描电镜观察[J]. 昆虫知识, 2010, 47(6):1174-1178. DOI: 10.7679/j.issn.2095-1353.2010.211. LIU X H, LUO Y Q, WANG J W, et al. Observations on the antennal sensilla of Adosomus sp. with scanning electron microscope[J]. Chinese Bulletin of Entomology, 2010, 47(6):1174-1178.
[7] 杨贵军, 张大治, 孙晶莹. 沟眶象触角感器的扫描电镜观察[J]. 昆虫知识, 2008, 45(6): 926-931. DOI:10.3969/j.issn.0452-8255.2008.06.016. YANG G J, ZHANG D Z, SUN J Y. Observation on antennal sensilla of Eucryptorrhynchus chinensis with scanning electron microscope[J]. Entomological Knowledge, 2008, 45(6): 926-931.
[8] 高宇, 陈宗懋, 孙晓玲. 茶丽纹象甲触角感器的扫描电镜观察[J]. 植物保护, 2013, 39(3): 45-49. DOI:10.3969/j.issn. 0529-1542.2013.03.009. GAO Y, CHEN Z M, SUN X L. Antennal sensilla of the tea weevil Myllocerinus aurolineatu[J]. Plant Protection, 2013, 39(3): 45-50.
[9] 黄俊浩, 吴时英, 高磊, 等. 中国新记录种——香樟齿喙象的鉴别与为害[J]. 浙江农林大学学报, 2014, 3(5):764-767. DOI:10.11833/j.issn. 2095-0756.2014.05.016. HUANG J H, WU S Y, GAO L, et al. Diagnosis and damage of weevil pest Pagiophloeus tsushimanus on camphor tree[J]. Journal of Zhejiang A & F University, 2014, 3(5):764-767.
[10] SCHNEIDER D. Insect Antennae[J]. Annual Review of Entomology, 1964, 9(1):103-122. DOI:10.1016/j. micron. 2014.07.011.
[11] 那杰, 于维熙, 李玉萍, 等. 昆虫触角感器的种类及其生理生态学意义[J]. 沈阳师范大学学报, 2008, 26(2): 213-216. DOI:10.3969/j.issn. 1673-5862.2008.02.026. NA J, YU W X, LI Y P, et al. The types of insects antennal sensilla and its physiological ecology significance[J]. Journal of Shenyang Normal University(Natural Science), 2008, 26(2): 213-216.
[12] ZUO T T, WANG M, ZHANG J, et al. Antennal Sensilla of Cryptorhynchus lapathi L.(Coleoptera: Curculionidae)[J]. Entomological News, 2015, 125(3): 199-213. DOI:10.3157/021.125.0309.
[13] SADI, TAUBAN D, RENOU M, et al. Structure and function of the antennal sensilla of the palm weevil Rhynchophorus palmarum(Coleoptera, Curculionidae)[J]. Journal of Insect Physiology, 2003, 49(9): 857-872. DOI:10.1016/S0022-1910(03)00137-9.
[14] 王玉刚, 张真, 王鸿斌, 等. 红脂大小蠹成虫触角扫描电镜的观察[J]. 林业科学研究, 2003, 16(6): 726-730. DOI:10.3321/j.issn:1001-1498.2003.06.012. WANG Y G, ZHANG Z, WANG H B, et al. Observation on antennal sensilla of red turpentine beetle(Dendroctonus valens)with scanning electron microscope[J]. Forest Research, 2003, 16(6): 726-730.
[15] MACKAY C A, SWEENEY J D, HILLIER N K. Morphology of antennal sensilla of the brown spruce longhorn beetle, Tetropium fuscum(Fabr.)(Coleoptera: Cerambycidae)[J]. Arthropod Structure & Development, 2014, 43(5): 469-475. DOI:10.1016/j.asd.2014.04.005.
[16] 刘晓梅, 凌冰, 赵旭伟, 等. 黄曲条跳甲触角感器的扫描电镜观察[J]. 环境昆虫学报, 2015, 37(1):68-76. DOI:10.3969/j.issn.1674-0858.2015.01.11. LIU X M, LING B, ZHAO X W, et al. Ultrastructure of antennal sensilla of Phyllotreta striolata Fabricius(Coleoptera: Alticinae)[J]. Journal of Environmental Entomology, 2015, 37(1):68-76.
[17] KAISSLING K. Chemo-electrical transduction in insect olfactory receptors[J]. Annual Review of Neuroscience, 1986, 9(1): 121-145. DOI:10.1146/annurev.ne.09.030186.001005.
[18] KANG G J, ZHU Z R, CHENG J A, et al. Antennal sensilla of parthenogenetic and bisexual Lissorhoptrus oryzophilus(Coleoptera: Curculionidae)[J]. Florida Entomologist, 2012, 95(1):8-15. DOI:10.1653/ 024.095.0102.
[19] ZHAO H Y, WANG Z Y, GAO Y, et al. Antennal morphology and sensillum distribution in Pissodes validirostris (Coleoptera: curculionidae)[J]. Indian Agricultural Research Journals, 2012, 46(1): 70-74. DOI: 10.1002/jemt.20920.
[20] ALMAAS T J, MUSTAPARTA H. Heliothis virescens: response characteristics of receptor neurons in sensilla trichodea type 1 and type 2[J]. Journal of Chemical Ecology, 1991, 17(5): 953-972. DOI: 10.1007/BF01395602.
[21] MUSTAPARTA H. Olfactory sensilla on the antennae of the pine weevil, Hylobius abietis[J]. Zeitschrift für Zellforschung und Mikroskopische Anatomie, 1973, 144(4): 559-571. DOI:10.1007/BF00307381.
[22] MUSTAPARTA H. Responses of single olfactory cells in the pine weevil Hylobius abietis L.(Col.: Curculionidae)[J]. Journal of Comparative Physiology, 1975, 97(4): 271-290. DOI:10.1007/BF00631966.
[23] MAIDA R, MAMELI M, MLLER B, et al. The expression pattern of four odorant-binding proteins in male and female silk moths, Bombyx mori[J]. Journal of Neurocytology, 2005, 34(1-2): 149-163. DOI:10.1007/ s11068-005-5054-8.
[24] BARTLET E, ROMANI R, WILLIAMS I H, et al. Functional anatomy of sensory structures on the antennae of Psylliodes chrysocephala L.(Coleoptera: Chrysomelidae)[J]. International Journal of Insect Morphology and Embryology, 1999, 28(4): 291-300. DOI:10.1016/j.asd.2014.04.005.
[25] LER B, RENARD S, ALLO M R, et al. Antennal sensilla and their possible functions in the host-plant selection behaviour of Phenacoccus manihoti (Matile-Ferrero)(Homoptera: Pseudococcidae)[J]. International Journal of Insect Morphology and Embryology, 1995, 24(4): 375-389. DOI:10.1016/0020-7322(95)00012-S.
[26] SKILBEEK C A, ANDERSON M. The fine structure of glandular units on the antennae of two species of the parasitoid, Aleochara(Coleoptera:Staphylinidae)[J]. International Journal of Insect Morphology and Embryology, 1994, 23(4): 319-328. DOI:10.1016/0020-7322(94)90028-0.
[27] MERIVEE E, RAHI M, BRESCIANI J, et al. Antennal sensilla of the click beetle, Limonius aeruginosus (Olivier)(Coleoptera: Elateridae)[J]. International Journal of Insect Morphology and Embryology, 1998, 27(4): 311-318. DOI: 10.1016/S0020-7322(98)00023-3.

基金

收稿日期:2016-06-07 修回日期:2017-04-13
基金项目:上海市松江区科技攻关项目(14SJGGNY11); 上海市绿化和市容管理局攻关项目(G161206)
第一作者:顾天滋(tianzigu@gmail.com),博士生。*通信作者:郝德君(djhao@njfu.edu.cn),教授。
引文格式:顾天滋,张丛丛,苏鹏,等. 香樟齿喙象成虫触角感器的微观特征分析[J]. 南京林业大学学报(自然科学版),2017,41(4):89-94.