淹水胁迫对湖北海棠生长及叶绿素荧光动力学的影响

doi:10.3969/j.issn.1000-2006.201703065

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (01): 35-40.doi: 10.3969/j.issn.1000-2006.201703065

淹水胁迫对湖北海棠生长及叶绿素荧光动力学的影响

张虎¹,曹福亮^2*, 范俊俊², 张往祥²

1. 江苏农林职业技术学院风景园林学院,江苏句容 212400; 2.南京林业大学林学院,江苏南京 210037

出版日期:2018-03-30 发布日期:2018-03-30
基金资助:
基金项目:江苏省科技厅重点研发计划(BE2016332); “十三五”国家科技支撑计划(2015BAD07B0104); 江苏省高职院校教师高级访问学者计划(2015FX026) 第一作者:张虎(315725756@qq.com),副教授。*通信作者:曹福亮(flcao126@126.com),教授,院士。

Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

ZHANG Hu¹, CAO Fuliang^2*, FAN Junjun², ZHANG Wangxiang²

1. College of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China; 2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2018-03-30 Published:2018-03-30

摘要/Abstract

摘要： 【目的】评价湖北海棠耐涝性,并从色素含量和叶绿素荧光动力学角度分析其耐涝机制。【方法】通过人工模拟淹水胁迫,对淹水胁迫过程中湖北海棠生长形态、叶片外观及叶绿素快相荧光动力学特征进行分析。【结果】①淹水处理显著抑制湖北海棠的生长,但淹水30～200 d苗木成活率仍为100%。②淹水后叶色转红,叶绿素与类胡萝卜素(Car)和花青素(Anth)的相对含量比值(m_Chl/m_Car+Anth)显著下降。③进行叶绿素荧光动力学观测发现,处理叶片在O相的初始荧光F_o显著高于对照,在P相的最大荧光F_m显著低于对照,且淹水后在J相处的可变荧光V_j升幅(3.4%～9.0%)大于K相处的可变荧光V_k升幅(3.8%～11.6%)。随着淹水时间的延长,叶片单位面积的反应中心数目(RC/CS_m)和PS Ⅱ电子受体库容量(S_m)显著降低,电子由Q_A向Q_B的传递过程受抑制,导致PSⅡ向PSⅠ的电子传递能力降低(Ψ_o和φ_Ro),最终表现为综合性能参数(PI_total)受到显著抑制,而叶片的热耗散(DI_o/CS_m)显著递增。【结论】淹水胁迫显著抑制湖北海棠生长,但并不致死,因此可认为其具备一定的耐水淹能力。虽然淹水对湖北海棠叶片PSⅡ电子受体侧的影响大于供体侧,但叶片可通过增加热耗散比及Car和Anth相对含量比例来降低激发能的产生,以及过剩激发能的伤害,从而提高其耐水淹能力。

Abstract: 【Objective】The goal of this study was to evaluate waterlogging tolerance of Malus hupehensis, and analyze the waterlogging resistance in terms of pigment relative content and chlorophyll fluorescence kinetics. 【Method】The growth, morphology, leaf appearance, and chlorophyll fluorescence characteristics of M. hupehensis during flooding were analyzed by artificial simulation of flooding stress.【Result】①The growth of M. hupehensis was significantly inhibited by flooding treatment, but the survival rate of seedlings was 100% after 30 days to 200 days flooding. ②The leaves gradually turned red, and the ratio of relative content of chlorophyll to carotenoids and anthocyanin(m_Chl/m_Car+Anth)decreased significantly after flooding. ③ The results of chlorophyll fluorescence kinetics showed that the initial fluorescence F_o(at the O phase)was significantly higher than that in the control, but the maximum fluorescence F_m(at the P phase)was significantly lower than that in the control. After the flooding, the increasing rate of the variable fluorescence V_j(3.4%-9.0%)compared with that of the control was larger than that of V_k(3.8%-11.6%). With prolongation of flooding stress, the number of reaction centers(RC/CS_m)and the capacity of PSⅡ(S_m)significantly decreased, and the transfer of electrons from Q_A to Q_B was inhibited, which resulted in reducing the ability of electronic transmission from PSⅡ to PSⅠ(Ψ_o and φ_Ro). Finally, as a result, the performance indexes(PI_total)significantly decreased. However, the value of DI_o/CS_m increased to reduce the generation of excitation energy.【Conclusion】Flooding significantly inhibited the growth of M. hupehensis, but no seedling died. Therefore, it could be considered that M. hupehensis had a certain waterlogging resistance. Although the effect on the electron acceptor of PSⅡwas greater than that of the donor, M. hupehensis could decrease the excitation energy to improve the flood resistance by increasing heat dissipation ratio, carotenoid and anthocyanin relative content.

中图分类号:

S718
Q945

张虎,曹福亮,范俊俊,等. 淹水胁迫对湖北海棠生长及叶绿素荧光动力学的影响[J]. 南京林业大学学报（自然科学版）, 2018, 42(01): 35-40.

ZHANG Hu, CAO Fuliang, FAN Junjun, ZHANG Wangxiang. Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 42(01): 35-40.DOI: 10.3969/j.issn.1000-2006.201703065 .

参考文献

[1] STRASSER R J, TSIMILLI-MICHAEL M, QIANG S, et al. Simultaneous in vivo recording of prompt and delayed fluorescence and 820 nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis[J]. Biochimica et Biophysica Acta, 2010, 1797(6/7): 1313-1326. DOI: 10.1016/j.bbabio.2010.03.008.
[2] GOLTOLTSEV V, ZAHARIEVA I, CHERNEV P, et al. Delayed fluorescence in photosynthesis[J]. Photosynthesis Research, 2009, 101(2/3): 217-232. DOI: 10.1007/s11120-009-9451-1.
[3] PAPAGEORGIOU E, GOVINDJEE G C. Chlorophyll a fluorescence: a signature of photosynthesis[J]. Journal of Plant Physiology, 2016, 163(6):689-690. DOI: 10.1016/j.jplph.2005.10.001.
[4] 李鹏民, 高辉远, STRASSER R J. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J]. 植物生理与分子生物学学报, 2005, 31(6): 559-566. DOI: 10.3321/j.issn:1671-3877.2005.06.001. LI P M, GAO H Y, STRASSER R J. Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study[J]. Journal of Plant Physiology and Molecular Biology, 2005, 31(6):559-566.
[5] ZHOU Z Q. The apple genetic resources in China: the wild species and their distributions, informative characteristics and utilisation[J]. Genet Res Crop Evol, 1999, 46(6): 599-609. DOI: 10.1023/A:1008747709534.
[6] 屈克义, 胡汉环, 杜远义, 等. 湖北海棠叶煎液药效学实验研究[J]. 时珍国医国药, 2000, 11(2): 107-108. DOI: 10.3969/j.issn.1008-0805.2000.02.006. QU K Y, HU H H, DU Y Y, et al. Experiment study of pharmacodynamics of Malus hupehensis decoction[J]. Lishizhen Medicne and Materia Medica Research, 2000, 11(2): 107-108.
[7] ZHANG W, FAN J, TAN Q, et al. Mechanisms underlying the regulation of root formation in Malus hupehensis, stem cuttings by using exogenous hormones[J]. Journal of Plant Growth Regulation, 2017, 36(1): 174-185. DOI: 10.1007/s00344-016-9628-8.
[8] 马丽清. 珠眉海棠与山定子耐盐机理的对比研究[D]. 北京: 中国农业大学, 2002. MA L Q. Comparative studies on salt tolerance mechanisms of Malus zumi and Malus baccata[D]. Beijing: China Agricultural University, 2002.
[9] 张坤玺. 苹果砧木幼苗耐碱性综合评价及营养元素积累的差异分析[D]. 杨凌: 西北农林科技大学, 2016. ZHANG K X. Alkali-tolerance of apple rootstock seedlings and the differences in nutrient elements accumulation[D]. Yanglin: Northwest Agriculture and Forestry University, 2016.
[10] 范晓丹, 刘飞, 王衍安, 等. 不同苹果砧木对缺锌胁迫的耐性评价[J]. 应用生态学报, 2015, 26(10): 3045-3052. DOI:10.13287/j.1001-9332.20150921.002. FAN X D, LIU F, WANG Y A, et al. Evaluation of zinc deficiency tolerance in different kinds of apple rootstocks[J]. Chinese Journal of Applied Ecology, 2015, 26(10): 3045-3052.
[11] 刘春风, 谢寅峰, 张往祥. 不同品种海棠对高温胁迫的生理响应[J]. 林业工程学报, 2015, 29(4):31-36. DOI:10.13360/j.issn.1000-8101.2015.04.007. LIU C F, XIE Y F, ZHANG W X. Physiological response of different crabapple varieties to high temperature stress[J]. Journal of Forestry Engineering, 2015, 29(4): 31-36.
[12] 刘春风, 刘少轩, 谢寅峰,等. 高温胁迫对观赏海棠形态和生长的影响[J]. 林业科技开发, 2013, 27(3):42-45. DOI: 10.3969/j.issn.1000-8101.2013.03.011. LIU C F, LIU S X, XIE Y F, et al. Effects of high temperature stress on morphology and growth of crabapple varieties[J]. China Forestry Science and Technology, 2013, 27(3): 42-45.
[13] 李萍萍. 观赏海棠品种(系)的抗寒特性研究[D]. 泰安: 山东农业大学, 2012. LI P P. Study of cold-resistance cultivars(colne)in crabapple(Malus spp.)[D]. Taian: Shandong Agricultural University, 2012.
[14] 徐颖. 海棠幼苗对干旱复水和NaCl胁迫的反应及其抗性评价[D]. 泰安: 山东农业大学, 2016. XU Y. Resistance evaluation and response of crabapple seedlings under drought and rehydration and NaCl solution stress[D]. Taian: Shandong Agricultural University, 2016.
[15] ZHAO H, YANG H. Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malus hupehensis Rehd[J]. Scientia Horticulturae, 2008, 116(4): 442-447. DOI: 0.1016/j.scienta.2008.02.017.
[16] MERZLYAK M N, SOLOVCHENKO A E, GITELSON A A.Reflectance spectral features and non-destructive estimation of chlorophyll, carotenoid and anthocyanin content in apple fruit[J]. Postharvest Biology and Technology, 2003, 27(2): 197-211. DOI: 10.1016/S0925-5214(02)00066-2.
[17] STRASSER R J, SRIVASTAVA A, TSIMILLI-MICHAEL M. The fluorescence transient as a tool to characterize and screen photosynthetic samples[M]. London: Publishers Taylor and Francis, 2000: 445-483.
[18] 曹福亮, 蔡金峰, 汪贵斌, 等. 淹水胁迫对乌桕生长及光合作用的影响[J]. 林业科学, 2010, 46(10): 57-61. DOI: 10.11707/j.1001-7488.20101009. CAO F L, CAI J F, WANG G B, et al. Effects of waterlogging stress on the growth and photosynthesis of Sapium sebiferum[J]. Scientia Silvae Sinicae, 2010, 46(10):57-61.
[19] 聂庆娟, 史宝胜, 孟朝,等. 不同叶色红栌叶片中色素含量、酶活性及内含物差异的研究[J]. 植物研究, 2008, 28(5): 599-602. NIE Q J, SHI B S, MENG C, et al. The enzyme activities, pigment and inclusion contents in different leaves color of Cotinus coggygria ‘Royal Purple' in autumn[J]. Bulletin of Botanical Research, 2008, 28(5):599-602.
[20] 张敏, 黄利斌, 周鹏,等. 榉树秋季转色期叶色变化的生理生化[J]. 林业科学, 2015, 51(8): 44-51. DOI: 10.11707/j.1001-7488.20150806. ZHANG M, HUANG L B, ZHOU P, et al. Physiological and biochemical changes in Zelkova serrata leaves during leaf color transformation in autumn[J]. Scientia Silvae Sinicae, 2015, 51(8): 44-51.
[21] 衣宁, 赵文倩, 刘倩, 等. 油松新生叶与老叶光合功能的比较[J]. 林业科技, 2014, 39(6):10-14. DOI: 10.3969/j.issn.1001-9499.2014.06.003. YI N, ZHAO W Q, LIU Q, et al. Comparation on photosynthetic capacity of new leaves and older leaves of Pinus tabuliformis[J]. Forestry Science and Technology, 2014, 39(6): 10-14.
[22] 杨小鑫, 吕运舟, 董筱昀, 等. ‘金焰彩栾'与黄山栾树光合特性比较[J]. 南京林业大学学报(自然科学版), 2016, 40(4): 74-80. DOI: 10.3969/j.issn.1000-2006.2016.04.012. YANG X X, LV Y Z, DONG X Y, et al. Photosynthetic characteristics of Koelreuteria bipinnata var. integrifoliola and its natural yellow mutant ‘Jinyan' [J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2016, 40(4):74-80.
[23] DEMMIG-ADAMS B, ADAMS W W. The role of xanthophyll cycle carotenoids in the protection of photosynthesis[J]. Trends in Plant Science, 1996, 1(1): 21-26. DOI: 10.1016/S1360-1385(96)80019-7.
[24] HUGHES N M, MORLEY C B, SMITH W K. Coordination of anthocyanin decline and photosynthetic maturation in juvenile leaves of three deciduous tree species[J]. New Phytologist, 2007, 175(4):675-685.
[25] STEYN W J, WAND S J E, HOLCROFT D M, et al. Anthocyanins in vegetative tissues: a proposed unified function in photoprotection[J]. New Phytologist, 2002, 155(3): 349-361.
[26] ZHANG L T, ZHANG Z S, GAO H Y, et al. Mitochondrial alternative oxidase pathway protects plants against photoinhibition by alleviating inhibition of the repair of photodamaged PSⅡ through preventing formation of reactive oxygen species in Rumex K-1 leaves[J]. Physiologia Plantarum, 2011, 143(4): 396-407. DOI: 10.1111/j.1399-3054.2011.01514.x
[27] 邱念伟, 周峰, 王颖, 等. 松树与杨树叶片叶绿素快相荧光动力学特征比较[J]. 林业科学, 2013, 49(3): 136-143. DOI: 10.11707/j.1001-7488.20130319. QIU N W, ZHOU F, WANG Y, et al. Comparison on characteristics of the fast chlorophyll fluorescence induction kinetics between Pinus species and Populus species[J]. Scientia Silvae Sinicae, 2013, 49(3): 136-143.
[28] 姚广, 高辉远, 王未未, 等. 铅胁迫对玉米幼苗叶片光系统功能及光合作用的影响[J]. 生态学报, 2009, 29(3): 1162-1169. DOI: 10.3321/j.issn:1000-0933.2009.03.012. YAO G, GAO H Y, WANG W W, et al. The effects of Pb-stress on functions of photosystems and photosynthetic rate in maize seedling leaves[J]. Acta Ecologica Sinica, 2009, 29(3): 1162-1169.
[29] LU C M, ZHANG J H. Heat-induced multiple effects on PSII in wheat plants[J]. Journal of Plant Physiology, 2000, 156(2): 259-265. DOI: 10.1016/S0176-1617(00)80315-6.
[30] CHEN H X, GAO H Y, AN S Z, et al. Dissipation of excess energy in mehler-peroxidase reaction in Rumex leaves during salt shock[J]. Photosynthetica, 2004, 42(1): 117-122.
[31] JIANG C D, JIANG G M, WANG X, et al. Increased photosynthetic activities and thermostability of photosystem II with leaf development of elm seedlings(Ulmus pumila)probed by the fast fluorescence rise OJIP[J]. Environmental and Experimental Botany, 2006, 58(1): 261-268. DOI: 10.1016/j.envexpbot.2005.09.007.

淹水胁迫对湖北海棠生长及叶绿素荧光动力学的影响

Effects of flooding stress on the growth and chlorophyll fluorescence kinetics of Malus hupehensis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	丛明珠, 刘琪璟, 孙震, 董淳超, 钱尼澎. 长白山北坡植物群落β多样性及其组分驱动因素分析[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 99-106.
[2]	曹荔荔, 阮宏华, 李媛媛, 倪娟平, 王国兵, 曹国华, 沈彩芹, 徐亚明. 不同林龄水杉人工林地表大型土壤动物群落特征比较研究[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 91-98.
[3]	张怡婷, 夏念和, 林树燕, 丁雨龙. 我国寒竹属空间分布特征及影响因素[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 107-114.
[4]	胡衍平, 刘卫东, 张珉, 陈明皋, 程勇, 魏志恒, 庞文胜, 吴际友. 山乌桕家系叶片叶色参数和色素含量及其解剖结构研究[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 123-133.
[5]	王一洁, 王璐冕, 丁真慧, 钱程, 曹加杰. 城市滨水绿地空间夏季微气候效应研究[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 233-241.
[6]	赵国扬, 洪波, 高俊平, 赵鑫, 黄洪峰, 徐彦杰. 菊属新品种‘雀欢’[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 254-255.
[7]	任佳辉, 高捍东, 陈哲楠, 李浩, 刘强, 陈澎军. 杂交新美柳苗对盐涝胁迫的生长和生理响应[J]. 南京林业大学学报（自然科学版）, 2025, 49(2): 57-66.
[8]	董亚文, 陈双林, 谢燕燕, 郭子武, 张景润, 汪舍平, 徐勇敢. 林下植被演替过程中毛竹和主要优势树种叶片建成成本变化特征[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 179-186.
[9]	徐薪璐, 孔淑鑫, 吕卓, 江帅君, 赵婉琪, 林树燕. 靓竹叶色表型叶片形态、结构与光合特性相关性研究[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 145-154.
[10]	曹永慧, 陈庆标, 周本智, 葛晓改, 王小明. 不同截雨干旱时间对毛竹叶片氮含量时空分布的影响[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 155-161.
[11]	隋夕然, 李军, 陈娟, 华军, 沈谦, 杨洪胜, 何前程, 李由, 王伟, 彭冶, 葛之葳, 张增信. 徐州市侧柏人工林群落不同演替阶段物种多样性变化[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 171-178.
[12]	尹华康, 张晋东, 黄金燕, 蒲冠桦, 毛泽恩, 周材权, 黄耀华, 付励强. 四川马边大风顶自然保护区大熊猫主食竹空间分布特征[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 187-193.
[13]	孔凡斌, 金晨涛, 徐彩瑶. 罗霄山地区生态系统服务与居民福祉耦合协调关系变化及其影响因素[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 245-254.
[14]	龚霞, 吴银明, 王海峰, 曾攀, 唐亚, 温铿, 焦文献. 花椒新品种‘蜀椒1号’[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 265-266.
[15]	吴桐, 王贤荣, 伊贤贵, 周华近, 陈洁, 李蒙, 陈祥珍, 高书成. 樱花新品种‘胭脂雪’[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 267-268.