南京林业大学学报(自然科学版) ›› 2019, Vol. 43 ›› Issue (01): 32-38.doi: 10.3969/j.issn.1000-2006.20186025
任 丽,董京祥,杨 洋,黄海娇,李慧玉*
出版日期:
2019-01-28
发布日期:
2019-01-28
基金资助:
REN Li,DONG Jingxiang,YANG Yang,HUANG Haijiao,LI Huiyu*
Online:
2019-01-28
Published:
2019-01-28
摘要: 【目的】研究BpTCP7启动子的表达模式,为深入分析BpTCP7基因功能提供一定参考。【方法】克隆了BpTCP7基因上游1 791 bp启动子序列,通过PLACE和Plant CARE网址对顺式作用元件进行分析,然后转入拟南芥,并分析BpTCP7启动子的组织表达特性及盐、旱胁迫应答反应。【结果】BpTCP7启动子序列中含有与细胞周期、开花、叶片发育、激素及胁迫响应等相关的顺式作用元件。该启动子在拟南芥中的表达模式呈现为营养生长阶段和生殖生长阶段均有表达,且在叶片中表达明显,与此同时,BpTCP7启动子在幼嫩、成熟叶片的边缘均有表达。与对照相比,NaCl、PEG胁迫诱导处理后BpTCP7基因表达量有升高的现象。【结论】BpTCP7基因参与白桦的叶片发育、激素应答、胁迫响应(盐、干旱)等生物学过程,对营养、生殖生长阶段各组织器官的发育也有一定的调控作用。
中图分类号:
任丽,董京祥,杨洋,等. 白桦BpTCP7基因启动子的克隆及表达分析[J]. 南京林业大学学报(自然科学版), 2019, 43(01): 32-38.
REN Li,DONG Jingxiang,YANG Yang,HUANG Haijiao,LI Huiyu. Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(01): 32-38.DOI: 10.3969/j.issn.1000-2006.20186025.
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