无机氮素形态对银杏苗期碳氮代谢的影响

doi:10.3969/j.issn.1000-2006.201807043

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (02): 197-202.doi: 10.3969/j.issn.1000-2006.201807043

无机氮素形态对银杏苗期碳氮代谢的影响

黄东静,曹福亮,汪贵斌^*

(南京林业大学南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037)

出版日期:2019-03-30 发布日期:2019-03-30
基金资助:
收稿日期:2018-07-19 修回日期:2018-12-11
基金项目:国家重点研发计划(2017YFD0600700)。
第一作者:黄东静(291051451@qq.com)。
*通信作者:汪贵斌(gbwang@njfu.com.cn),教授,ORCID(0000-0002-9987-6496)。

Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba

HUANG Dongjing, CAO Fuliang, WANG Guibin^*

(Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry,Nanjing Forestry University, Nanjing 210037, China)

Online:2019-03-30 Published:2019-03-30

摘要/Abstract

摘要： 【目的】研究不同氮素形态配比施肥对银杏碳氮代谢的影响,为银杏合理施肥提供理论依据。【方法】以1年生银杏实生苗为材料,采用温室盆栽试验,设置5种不同铵硝比[m(NH⁺₄-N):m(NO^-₃-N)]处理,分别为T₁(100:0)、T₂(75:25)、T₃(50:50)、T₄(25:75)和T₅(0:100),分析了不同处理下银杏叶片全氮、可溶性蛋白、游离氨基酸、全碳、可溶性糖、淀粉和蔗糖含量以及淀粉酶(AMS)、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和谷氨酸合成酶(GOGAT)活性的变化规律。【结果】随着NO^-₃-N比例的增加,银杏幼苗叶片碳氮代谢物含量和相关酶活性总体上(除可溶性蛋白含量)呈先增后减的变化趋势。碳氮代谢物方面,T₃处理的蔗糖和游离氨基酸含量最高,T₄处理的全氮、全碳、可溶性糖、可溶性蛋白、淀粉和蔗糖含量均最高,而T₁处理除可溶性蛋白含量外,剩余碳氮代谢物的含量均最低。酶活性方面,T₃处理的SS和SPS活性最高,T₄处理的AMS、NR、GS和GOGAT活性均最高,而T₁处理的AMS、SS和NR活性均最低,T₅处理的SPS、GS和GOGAT活性均最低。总体上,T₃和T₄处理碳氮代谢物含量和酶活性均显著高于其他处理,但T₃与T₄处理间无显著差异。【结论】NH⁺₄-N和NO^-₃-N混合施用增强了银杏的碳氮代谢能力,在当前土壤条件下,m(NH⁺₄-N):m(NO^-₃-N)为25:75时效果最佳,在生产中应根据实际情况进行适当的调整。

Abstract: 【Objective】The effects of nitrogen form and ratio on carbon-nitrogen metabolism and related enzyme activities in the leaves of Ginkgo biloba seedlings were investigated to provide a theoretical basis for N fertilizer management of Ginkgo biloba. 【Method】Using 1-year-old seedlings of Ginkgo biloba as test materials, a greenhouse pot experiment was performed with 5 ratios of m(NH₄⁺-N):m(NO₃-N), namely T₁(100:0), T₂(75:25), T₃(50:50), T₄(25:75), and T₅(0:100). We analyzed the content of total nitrogen, soluble protein, free amino acid, total carbon, soluble sugar, starch and sucrose, and the activity of amylase(AMS), sucrose synthetase(SS), sucrose phosphate synthase(SPS), nitrate reductase(NR), glutamine synthetase(GS), and glutamine synthetase(GOGAT)in the Ginkgo biloba seedlings under different treatments. 【Result】The carbon-nitrogen metabolites(except soluble protein content)and related enzyme activities in the leaves of Ginkgo biloba increased initially and then decreased, with an increase in the NO₃^--N ratio. In terms of carbon-nitrogen metabolism, the content of sucrose and free amino acids were the highest in the T₃ group, and the content of total nitrogen, total carbon, soluble sugar, soluble protein, starch, and sucrose were the highest in the T₄ group. The content of carbon-nitrogen metabolism indicators, except for soluble protein content, were the lowest in the T₁ group. In terms of enzyme activity, SS and SPS activities were the highest in the T₃ group, and AMS, NR, GS and GOGAT activities were the highest in the T₄ group. AMS, SS, and NR activities were the lowest in the T₁ group, and SPS, GS and GOGAT activities were the lowest in the T₅ group. Overall, the contents of carbon-nitrogen metabolites and related enzyme activities in T₃ and T₄ groups were significantly higher than that in other groups, but no significant differences were observed between the T₃ and T₄ groups. 【Conclusion】The effects of NH₄⁺-N and NO₃^--N mixed supply on the carbon-nitrogen metabolism of Ginkgo biloba were significantly higher than those of the single supply. The effect of 25:75 m(NH₄⁺-N):m(NO₃^--N)was the best, and appropriate adjustments should be performed on the basis of the conditions.

中图分类号:

S792.95

黄东静,曹福亮,汪贵斌. 无机氮素形态对银杏苗期碳氮代谢的影响[J]. 南京林业大学学报（自然科学版）, 2019, 43(02): 197-202.

HUANG Dongjing, CAO Fuliang, WANG Guibin. Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba [J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(02): 197-202.DOI: 10.3969/j.issn.1000-2006.201807043.

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无机氮素形态对银杏苗期碳氮代谢的影响

Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba

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