碳源对不同产地香樟体细胞胚胎发生及植株再生的影响

doi:10.3969/j.issn.1000-2006.201904002

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南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (1): 54-62.doi: 10.3969/j.issn.1000-2006.201904002

碳源对不同产地香樟体细胞胚胎发生及植株再生的影响

荆茹月¹(), 王佩兰¹, 黄振², 何林骏¹, 李志辉¹^,^*()

1.中南林业科技大学林学院,湖南长沙 410004
2.四川省林业科学院,四川成都 610081

收稿日期:2019-04-01 修回日期:2019-06-20 出版日期:2020-02-08 发布日期:2020-02-02
通讯作者: 李志辉
作者简介:荆茹月( 326040597@qq.com)。
基金资助:
国家重点研发计划(2016YFD06006005);中南林业科技大学研究生科技创新基金(20183030)

Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

JING Ruyue¹(), WANG Peilan¹, HUANG Zhen², HE Linjun¹, LI Zhihui¹^,^*()

1. College of Forestry, Central South Forestry University of Science and Technology, Changsha 410004, China
2. Sichuan Academy of Forestry Sciences,Chengdu 610081, China

Received:2019-04-01 Revised:2019-06-20 Online:2020-02-08 Published:2020-02-02
Contact: LI Zhihui

摘要/Abstract

摘要：

【目的】 探究渗透调节物质种类及其不同浓度对香樟体细胞胚胎发生及植株再生的影响,为提高香樟体胚诱导率、优化体细胞胚胎发生体系奠定理论基础。【方法】 以7月初在湖南省郴州、长沙、娄底及怀化市采集的香樟(Cinnamomum camphora L.)的未成熟合子胚为试验材料,分析不同碳源种类、浓度及组合方式对香樟体胚诱导、芽萌发、生根等方面的影响。【结果】 适合香樟的体胚诱导培养基为MS+1.0 mg/L的6-苄氨基腺嘌呤(6-BA)+0.3 mg/L的2,4-二氯苯氧乙酸(2,4-D)+1.0 mg/L 链霉素+15 g/L 蔗糖+30 g/L 山梨醇+6.5 g/L 琼脂+700 mg/L水解酪蛋白;增殖培养基为MS+15 g/L 蔗糖+30 g/L 山梨醇+6.5 g/L 琼脂+700 mg/L 水解酪蛋白;芽萌发培养基为MS+0.1 mg/L 苯基噻二唑基脲(TDZ)+0.5 mg/L 萘乙酸(NAA)+0.1 mg/L 吲哚丁酸(IBA)+15 g/L 蔗糖+15 g/L 山梨醇+6.5 g/L 琼脂;生根培养基为MS+1.0 mg/L IBA+15 g/L 蔗糖+15 g/L 山梨醇+6.5 g/L 琼脂。【结论】 经过改良,添加15 g/L蔗糖和30 g/L山梨醇的组合碳源,香樟体胚的诱导率明显提高;添加15 g/L蔗糖和15 g/L山梨醇的组合碳源处理的新植株生根条数多、主根明显,生长状况更优。

关键词: 香樟, 碳源, 体胚诱导率, 芽萌发率, 生根率

Abstract:

【Objective】 In this study, we investigated the effects of somotic adjustment substances on somatic embryogenesis and regeneration of Cinnamomum camphora L. plants, which will provide a foundation for enhancing the induction rate of somatic embryogenesis and optimizing a somatic embryogenesis system for this species. 【Method】 The immature zygotic embryos of C. camphora collected in early July in Chenzhou, Changsha, Loudi and Huaihua City were used as materials for examining the effects of different carbon source types, concentrations, and combinations on somatic embryogenesis. The somatic embryo induction rate, germination rate, and rooting rate of plants were determined under different conditions.【Result】 The suitable medium for somatic embryo induction was MS medium+1.0 mg/L 6-benzylaminopurine (6+BA)+0.3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D)+1.0 mg/L streptomycin+15 g/L sucrose+30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable proliferation medium was MS+15 g/L sourse +30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable medium for bud germination was murashige and skoog medium+0.1 mg/L thidiazuron (TDZ)+0.5 mg/L naphthylacetic acid (NAA)+0.1 mg/L 3-indolebutyric acid (IBA)+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar; and a suitable medium for rooting was MS+1.0 mg/L IBA+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar. 【Conclusion】 As a consequence of modification, somatic embryo induction was significantly improved by adding 15 g/L sucrose and 30 g/L sorbitol as combined carbon sources, and the new plants thus generated had a larger number of rooting strips, obvious main roots, and better growth characteristics.

Key words: Cinnamomum camphora L., carbon sources, somatic embryo induction rate, bud germination rate, rooting rate

中图分类号:

S792.23

荆茹月,王佩兰,黄振,等. 碳源对不同产地香樟体细胞胚胎发生及植株再生的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(1): 54-62.

JING Ruyue, WANG Peilan, HUANG Zhen, HE Linjun, LI Zhihui. Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(1): 54-62.DOI: 10.3969/j.issn.1000-2006.201904002.

图/表 9

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碳源种类 types of carbon sources	体胚诱导率/% induction rate of somatic embryo
碳源种类 types of carbon sources	郴州 Chenzhou	长沙 Changsha	怀化 Huaihua	娄底 Loudi
蔗糖sucrose	72.9±2.6 aC	83.7±1.1 aA	77.4±1.5 aB	69.1±1.3 aD
葡萄糖glucose	53.2±2.7 bB	59.7±1.9 bA	45.2±2.1 bC	44.9±1.6 bC
麦芽糖maltose	0.0	10.3±3.9 eA	3.5±1.7 dB	0.0
山梨醇sorbitol	33.3±1.5 cC	45.3±1.3 cA	46.4±1.9 bA	37.9±1.1 cB
甘露醇mannitol	31.9±2.4 cB	44.7±1.5 cA	44.5±2.3 bA	32.5±3.2 dB
海藻糖trehalose	11.2±1.6 dB	22.3±2.6 dA	18.7±3.9 cA	19.7±1.3 eA

因素类型 factor type	factor 系数 coefficient	标准差 standard deviation	标准化系数 standard coefficient	t	P
常量constant	67.786	7.339		9.236	<0.001^***
产地different original location	-0.425	2.055	-0.020	-0.207	0.837
碳源种类types of carbon sources	-8.251	1.346	-0.594	-6.132	<0.001^***
整体模型integral model	R²=0.353 F (2,69) =18.82		R²=0.334 P<0.001^***

混合碳源质量浓度/(g·L^-1) concentration of two mixed carbon sources			体胚诱导率/% induction rate of somatic embryo
蔗糖 source	山梨醇 sorbitol	甘露醇 mannitol	体胚诱导率/% induction rate of somatic embryo
45(CK)			84.1±1.3 c
15	15		87.7±2.3 b
15	30		92.9±1.7 a
15	45		55.3±1.4 d
15	60		31.9±2.5 e
45(CK)			84.1±1.3 b
15		15	85.4±1.7 b
15		30	90.8±1.1 a
15		45	55.1±3.2 c
15		60	30.7±2.5 d

碳源种类 types of carbon sources	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
蔗糖source	46.3±1.4 a	83.7±1.4 a
葡萄糖glucose	35.4±1.9 b	81.3±3.6 a
麦芽糖maltose	0.0	0.0
山梨醇sorbitol	45.3±1.3 a	83.3±1.6 a
甘露醇mannitol	44.4±1.9 a	82.0±2.9 a
海藻糖trehalose	19.4±3.2 c	40.3±1.2 b

蔗糖质量浓度 / (g·L^-1) concentration of sucrose	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
10	41.2±5.7 b	63.3±2.1 b
20	53.3±1.2 a	85.4±4.2 a
30	46.3±1.4 b	83.7±1.4 a

碳源对不同产地香樟体细胞胚胎发生及植株再生的影响

Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

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两种混合碳源质量浓度/(g·L^-1) concentration of two mixed carbon sources			体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
蔗糖 sucrose	山梨醇 sorbitol	甘露醇 mannitol	体胚芽萌发率/% bud germination rate	体胚生根率/% rooting rate
45(CK)			53.3±1.2 a	85.4±4.2 a
15	15		54.7±1.7 a	86.9±3.7 a
15	30		47.3±1.7 b	84.3±3.5 a
15	45		47.2±3.6 b	84.2±3.2 a
45(CK)			53.3±1.2 a	85.4±4.2 a
15		15	53.6±2.7 a	86.0±2.3 a
15		30	46.8±1.6 b	83.9±1.6 a
15		45	41.9±2.6 c	64.4±2.3 b

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