4个北美冬青品种苗对低温胁迫的生理响应及抗寒性比较

doi:10.3969/j.issn.1000-2006.202003059

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (5): 34-40.doi: 10.3969/j.issn.1000-2006.202003059

所属专题：色叶树木与木本花卉专题

• 专题报道Ⅱ(执行主编方升佐) • 上一篇下一篇

4个北美冬青品种苗对低温胁迫的生理响应及抗寒性比较

马娟娟(), 赵斌, 陈颖^*(), 凌熙晨, 俞婕, 陈茜

南京林业大学生物与环境学院,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2020-03-19 修回日期:2020-05-15 出版日期:2020-10-30 发布日期:2020-10-30
通讯作者: 陈颖
基金资助:
江苏省科技计划重点项目(BE2017375);江苏高校优势学科建设工程项目(PAPD);南京林业大学大学生创新训练计划项目(2019NFUSPITP0448)

Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance

MA Juanjuan(), ZHAO Bin, CHEN Ying^*(), LING Xichen, YU Jie, CHEN Xi

Co-Innovation Center for the the Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

Received:2020-03-19 Revised:2020-05-15 Online:2020-10-30 Published:2020-10-30
Contact: CHEN Ying

摘要/Abstract

摘要：

【目的】探究不同品种北美冬青的抗寒性,为北美冬青新品种苗木的推广和研究提供参考。【方法】以4个引进的北美冬青(Ilex verticillata)品种‘Red Sprite’、‘Gray’、‘Oosterwijk’和‘Winter Gold’2年生扦插苗枝条为试验材料,通过0、-7、-17、-27 ℃低温逐级降温分别处理24 h,研究4个品种北美冬青对低温胁迫的生理响应及抗寒性差异。【结果】4个品种的相对电导率和MDA(丙二醛)含量均随着温度的降低而增加,都在-27 ℃时达到最高。‘Red Sprite’、‘Gray’、‘Oosterwijk’和‘Winter Gold’的低温半致死温度(LT50)分别为-26.38、-24.26、-29.19和-20.04 ℃。随着处理温度的降低,渗透调节物质可溶性糖、可溶性蛋白及脯氨酸含量在处理温度>-17 ℃时都出现增加(‘Winter Gold’的可溶性糖含量最高点在-27 ℃),但各品种各指标峰值出现的温度点不同。H₂O₂含量与SOD(超氧化物歧化酶)、POD(过氧化物酶)酶活性同时在-17 ℃时达到最高,而CAT(过氧化氢酶) 酶活性在-27 ℃时急剧增加,说明3种酶对活性氧的清除存在温度效应。【结论】4个品种中‘Oosterwijk’与‘Gray’的抗寒性在整体水平上强于其他2个品种,通过隶属函数和权重综合分析,得出4个北美冬青品种的抗寒能力由强到弱依次为:‘Oosterwijk’、‘Gray’、‘Red Sprite’、‘Winter Gold’。

关键词: 北美冬青品种, 低温胁迫, 生理响应, 抗寒性评价

Abstract:

【Objective】 Investigating the cold resistance of four Ilex verticillata varieties provides a guidance and a reference for seedling promotion and research of these new varieties. 【Method】The physiological responses of four I. verticillata varieties (‘Red Sprite’, ‘Gray’, ‘Oosterwijk’ and ‘Winter Gold’) to low temperature stress were studied by exposing their branches to temperature treatments of 0 ℃, -7 ℃, -17 ℃ and -27 ℃ for 24 h. Cold resistance of the four varieties was compared. 【Result】The relative conductivity and malondialdehyde content of the four varieties increased with a decrease in temperature, and reached a maximum at -27 ℃. The low temperature semi-lethal temperatures for ‘Red Sprite’, ‘Gray’, ‘Oosterwijk’ and ‘Winter Gold’ were -26.38 ℃, -24.26 ℃, -29.19 ℃ and -20.04 ℃, respectively. Associated with the decrease in treatment temperature, there was an increase in the soluble sugar, soluble protein, and proline contents and osmotic regulators at > -17 ℃ (the soluble sugar of ‘Winter Gold’ reached its highest point at -27 ℃); however, the appearance of peak temperature for each variety was different. The H₂O₂ content and the activity of superoxide dismutase and peroxidase all reached their highest value at -17 ℃. The activity of the catalase enzyme increased sharply at -27 ℃, indicating that the three enzymes had a temperature effect on the scavenging of reactive oxygen species.【Conclusion】The cold resistance of ‘Oosterwijk’ and ‘Gray’ was stronger than that of the other two varieties as a whole. Through the comprehensive analysis of membership function and weight value, the order of cold resistance of the four I. verticillata varieties, from strong to weak was: ‘Oosterwijk’ > ‘Gray’ > ‘Red Sprite’ > ‘Winter Gold’.

Key words: Ilex verticillata varieties, low temperature stress, physiological response, cold resistance evaluation

中图分类号:

S688

马娟娟,赵斌,陈颖,等. 4个北美冬青品种苗对低温胁迫的生理响应及抗寒性比较[J]. 南京林业大学学报（自然科学版）, 2020, 44(5): 34-40.

MA Juanjuan, ZHAO Bin, CHEN Ying, LING Xichen, YU Jie, CHEN Xi. Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(5): 34-40.DOI: 10.3969/j.issn.1000-2006.202003059.

图/表 6

图1

表1

图2

图3

表2

表3

参考文献 27

[1]	余有祥, 周正宝, 徐旻昱 , 等. ‘奥斯特’北美冬青繁育技术[J]. 中国花卉园艺, 2012(14):32-35.
	YU Y X, ZHOU Z B, XU M Y , et al. Breeding technology of Ilex verticillata ‘Oosterwijk’[J]. China Flowers Hortic, 2012(14):32-35.
[2]	陈茜, 周之涵, 王瑞琪 , 等. 低温处理对北美冬青叶片的抗氧化能力的影响[J]. 热带亚热带植物学报, 2016,24(6):689-695.
	CHEN X, ZHOU Z H, WANG R Q , et al. Effect of low temperature stress on antioxidant ability in Ilex verticillata leaves[J]. Journal of Tropical and Subtropical Botany, 2016,24(6):689-695. 10.11926/j.issn.1005-3395.2016.06.013.
[3]	王伟丽, 何立平, 余敏芬 , 等. 12个北美冬青品种的ISSR亲缘关系分析[J]. 浙江农林大学学报, 2018,35(4):612-617.
	WANG W L, HE L P, YU M F , et al. Phylogenetic relationships among 12 cultivars of Ilex verticillata based on ISSR molecular markers[J]. J Zhejiang A F Univ, 2018,35(4):612-617.
[4]	查琳, 袁紫倩, 董建华 , 等. ‘奥斯特’北美冬青在我国的区域性引种试验[J]. 林业科技开发, 2015,29(6):80-82.
	ZHA L, YUAN Z Q, DONG J H , et al. Regional introduction experiment of Ilex verticillata ‘Oosterwijk’[J]. China For Sci Technol, 2015,29(6):80-82. DOI: 10.13360/j.issn.1000-8101.2015.06.020.
[5]	姚丽娟, 周秀兰, 杨燕萍 , 等. 温州地区北美冬青栽培适应性观察[J]. 农业科技通讯, 2016(1):223-224.
	YAO L J, ZHOU X L, YANG Y P , et al. Adaptability of cultivation of Ilex verticillata in Wenzhou area[J]. Bull Agric Sci Tech, 2016(1):223-224.
[6]	吴自光, 孙新新, 翟光耀 , 等. 临沂地区北美冬青引种适应性研究[J]. 现代农业科技, 2017(16):124-125.
	WU Z G, SUN X X, ZHAI G Y , et al. Study on the adaptability of introduction of Ilex verticillata in Linyi area[J]. Mod Agric Sic Technol, 2017(16):124-125.
[7]	李鸿杰, 雷颖 . 甘肃南部北美冬青引种适应性试验研究[J]. 林业科技通讯, 2019(1):39-42.
	LI H J, LEI Y . Study on introduction experiment of Ilex verticillatain in south of Gansu Province[J]. For Sci Technol, 2019 ( 1):39-42. DOI: 10.13456/j.cnki.lykt.2018.10.10.0008.
[8]	张小莉, 王鹏程, 宋纯鹏 . 植物细胞过氧化氢的测定方法[J]. 植物学报, 2009,44(1):103-106.
	ZHANG X L, WANG P C, SONG C P . Methods of detecting hydrogen peroxide in plant cells[J]. Chin Bull Bot, 2009,44(1):103-106.
[9]	李合生 . 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
	LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000.
[10]	梁锁兴, 席海源, 王文平 , 等. 电解质渗出率配合Logistic方程鉴定7个平欧杂种榛品种(系)的抗寒性[J]. 农学学报, 2017,7(4):34-38.
	LIANG S X, XI H Y, WANG W P , et al. Determination of cold-resistance for 7 flat-European hazel cultivars through electrical permeation rate combining Logistic equation[J]. J Agric, 2017,7(4):34-38.
[11]	王国霞, 王会鱼, 李春阁 , 等. 取样部位、时间对植物高温半致死温度的影响[J]. 福建林业科技, 2019,46(1):45-51.
	WANG G X, WANG H Y, LI C G , et al. Analysis of the influence of sampling site and time on the determination of semilethal high temperature of plants[J]. J Fujian For Sci Technol, 2019,46(1):45-51. DOI: 10.13428/j.cnki.fjlk.2019.01.010
[12]	刘旭梅, 赵冰, 申惠翡 , 等. 低温胁迫下二十个杜鹃花品种的抗寒性评价[J]. 北方园艺, 2017(5):60-66.
	LIU X M, ZHAO B, SHEN H F , et al. Comprehensive evaluation of cold resistance of twenty Rhododendron cultivars under cold stress[J]. North Hortic, 2017(5):60-66. DOI: 10.11937/bfyy.201705015
[13]	MACHADO R D, CHRISTOFF A P, LOSS-MORAIS G , et al. Comprehensive selection of reference genes for quantitative gene expression analysis during seed development in Brassica napus[J]. Plant Cell Rep, 2015,34(7):1139-1149. DOI: 10.1007/s00299-015-1773-1. doi: 10.1007/s00299-015-1773-1 pmid: 25721200
[14]	陈洁, 金晓玲, 宁阳 , 等. 3 种含笑属植物抗寒生理指标的筛选及评价[J]. 河南农业科学, 2016,45(2) : 113-118.
	CHEN J, JIN X L, NING Y , et al. Identification and comprehensive evaluation of cold resistance indexes of three Michelia plants[J]. Journal of Henan Agricultural Sciences, 2016,45(2) : 113-118. DOI: 10. 15933/j. cnki. 10043268.
[15]	LIU Y S, GENG J C, SHA X Y , et al. Effect of Rhizobium symbiosis on low-temperature tolerance and antioxidant response in alfalfa (Medicago sativa L.)[J]. Front Plant Sci, 2019,10:538. DOI: 10.3389/fpls.2019.00538. doi: 10.3389/fpls.2019.00538 pmid: 31114600
[16]	池敏杰, 刘育梅 . 3 种番荔枝属植物对低温胁迫的生理响应及抗寒性评价[J]. 2019,48(4):339-342.
	CHI M J, LIU Y M . Physiological response and cold resistance evaluation of three species of Annona to low temperature stress[J]. Subtropical Plant Science, 2019,48(4):339-342. DOI: 10.3969/j.issn.1009-7791.2019.04.006.
[17]	CAO S F, CAI Y T, YANG Z F , et al. MeJA induces chilling tolerance in loquat fruit by regulating proline and γ-aminobutyric acid contents[J]. Food Chem, 2012,133(4):1466-1470. DOI: 10.1016/j.foodchem.2012.02.035.
[18]	BAO G Z, AO Q, LI Q Q , et al. Physiological characteristics of Medicago sativa L. in response to acid deposition and freeze-thaw stress[J]. Water Air Soil Pollut, 2017,228(9):376. DOI: 10.1007/s11270-017-3561-8.
[19]	刘艳菊, 周丽霞, 曹红星 . 低温胁迫下不同浓度ABA对4个油棕新品种幼苗生理特性的影响[J]. 热带作物学报, 2020,41(6):1124-1131.
	LIU Y J, ZHOU L X, CAO H X . Effects of exogenous ABA on physiology of 4 oil Palm new varieties under cold stress[J]. Chinese Journal of Tropical Crops, 2020,41(6):1124-1131.
[20]	曹阳, 张旭艳 . 4种常绿阔叶植物叶片抗寒性研究[J]. 山西农业科学, 2019,47(3):334-336.
	CAO Y, ZHANG X Y . Study on cold resistance of four evergreen broad-leaved plant leaves[J]. J Shanxi Agric Sci, 2019,47(3):334-336. DOI : 10.3969/j.issn.1002-2481.2019.03.10.
[21]	李文明, 魏一粟, 钱燕萍 , 等. 5种酢浆草属植物对低温胁迫的生理响应及抗寒性评价[J]. 东北林业大学学报, 2017,45(7):28-33.
	LI W M, WEI Y S, QIAN Y P , et al. Evaluation of cold resistance and physiological response to low temperature on five kinds of Oxalis plants[J]. J Northeast For Univ, 2017,45(7):28-33. DOI: 10.13759/j.cnki.dlxb.2017.07.006
[22]	原慧芳, 谢江, 周会平 , 等. 不同橡胶树品种耐寒性指标比较及综合评价[J]. 植物资源与环境学报, 2018,27(4):72-80.
	YUAN H F, XIE J, ZHOU H P , et al. Comparison on cold tolerance indexes of different cultivars of Hevea brasiliensis and comprehensive evaluation [J]. J Plant Resour Environ, 2018,27(4):72-80. DOI : 10.3969/j.issn.1674-7895.2018.04.08.
[23]	方仁, 安振宇, 黄伟雄 , 等. 6个番荔枝品种在自然低温条件下的抗寒性比较[J]. 江苏农业科学, 2017,45(7):136-138.
	FANG R, AN Z Y, HUANG W X , et al. Comparison of cold resistance of 6 cherimoya cultivars under natural low temperature[J]. Jiangsu Agric Sci, 2017,45(7):136-138. DOI: 10.15889/j.issn.1002-1302.2017.07.036.
[24]	STEINDAL A L H, RØDVEN R, HANSEN E , et al. Effects of photoperiod,growth temperature and cold acclimatisation on glucosinolates,sugars and fatty acids in kale[J]. Food Chem, 2015,174:44-51. DOI: 10.1016/j.foodchem.2014.10.129. doi: 10.1016/j.foodchem.2014.10.129 pmid: 25529650
[25]	AIRAKI M, LETERRIER M, MATEOS R M , et al. Metabolism of reactive oxygen species and reactive nitrogen species in pepper (Capsicum annuum L.) plants under low temperature stress[J]. Plant Cell Environ, 2012,35(2):281-295. DOI: 10.1111/j.1365-3040.2011.02310.x. doi: 10.1111/j.1365-3040.2011.02310.x pmid: 21414013
[26]	CLEMENTE-MORENO M J, OMRANIAN N, SÁEZ P L , et al. Low-temperature tolerance of the Antarctic species Deschampsia antarctica:a complex metabolic response associated with nutrient remobilization[J]. Plant Cell Environ, 2020,13737:1-18. DOI: 10.1111/pce.13737.
[27]	DHARSHINI S, HOANG N V, MAHADEVAIAH C , et al. Root transcriptome analysis of Saccharum spontaneum uncovers key genes and pathways in response to low-temperature stress[J]. Environ Exp Bot, 2020,171:103935. DOI: 10.1016/j.envexpbot.2019.103935.

品种 varieties	Logistic拟合方程 fitting curve equation of Logistic	半致死温度/℃ LT₅₀	R²
‘Red Sprite’	y=100/(1+1.642e^0.019^x)	-26.4	0.952
‘Gray’	y=100/(1+2.173e^0.032^x)	-24.3	0.969
‘Oosterwijk’	y=100/(1+1.692e^0.018^x)	-29.2	0.976
‘Winter Gold’	y=100/(1+2.140e^0.038^x)	-20.0	0.945

品种 varieties	REC	MDA	H₂O₂	SS	SP	Pro	SOD	POD	CAT	隶属函数平均值 appraisal average value
‘Red Sprite’	0.43	0.87	0.81	0.52	0.39	0.51	0.29	0.17	0.43	0.49
‘Gray’	0.46	0.76	0.70	0.46	0.42	0.67	0.20	0.10	0.44	0.47
‘Oosterwijk’	0.50	0.21	0.75	0.46	0.85	0.65	0.29	0.70	0.32	0.52
‘Winter Gold’	0.42	0.43	0.75	0.44	0.35	0.29	0.15	0.19	0.23	0.36

品种 varieties	抗寒性指数(D) cold resistance index																	综合评价值 comprehensive assessment
品种 varieties	REC	MDA		H₂O₂		SS		SP		Pro		SOD		POD		CAT		综合评价值 comprehensive assessment
‘Red Sprite’	0.034		0.049		0.129		0.038		0.039		0.051		0.010		0.037		0.076	0.549
‘Gray’	0.039		0.020		0.211		0.067		0.040		0.051		0.005		0.008		0.077	0.605
‘Oosterwijk’	0.013		0.020		0.065		0.097		0.117		0.016		0.011		0.025		0.110	0.665
‘Winter Gold’	0.036		0.017		0.146		0.036		0.022		0.042		0.007		0.028		0.045	0.461

4个北美冬青品种苗对低温胁迫的生理响应及抗寒性比较

Physiological responses of seedlings of four Ilex verticillata varieties to low temperature stress and a comparison of their cold resistance

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 27

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	郭伟, 韩秀, 张利, 王迎, 杜辉, 燕语, 孙忠奎, 张林, 李国华, 罗磊. 青檀扦插苗对不同氮素水平的形态、光合生理响应和转录组分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 87-96.
[2]	洪震, 刘术新, 洪琮浩, 雷小华. 5种造林树种对干旱胁迫的抗性应答[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 111-119.
[3]	张庆, 魏树和, 代惠萍, 贾根良. 硒对茶树镉毒害的缓解作用研究[J]. 南京林业大学学报（自然科学版）, 2020, 44(1): 200-204.
[4]	董珊珊,李宁冉,杨海燕,吴文龙,闾连飞,李维林. 蓝莓根系对土壤锰胁迫的生理响应[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 169-174.
[5]	张宇,陈肃,高源,黄海娇. BpMYB4基因在白桦中的遗传转化及低温胁迫应答反应[J]. 南京林业大学学报（自然科学版）, 2019, 43(01): 25-31.
[6]	周余华,梁有旺,彭方仁. 低温胁迫下克恩氏冬青幼苗叶片差异蛋白质分析[J]. 南京林业大学学报（自然科学版）, 2017, 41(06): 187-192.
[7]	张衡锋,韦庆翠,汤庚国,陆小青. 7种冬青对苯气体胁迫的生理响应[J]. 南京林业大学学报（自然科学版）, 2015, 39(02): 174-178.
[8]	刘强,王占武,周晓梅. 两种枸杞对NaHCO₃胁迫的抗性生理响应[J]. 南京林业大学学报（自然科学版）, 2014, 38(06): 165-169.
[9]	陈容,张丽,曹颖,卢学琴,胡尚连,段宁. 低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达[J]. 南京林业大学学报（自然科学版）, 2014, 38(04): 39-44.
[10]	闫绍鹏,杨瑞华,王秋玉. 低温胁迫对欧美杂种山杨无性系的生理影响[J]. 南京林业大学学报（自然科学版）, 2013, 37(06): 161-164.
[11]	赵明明，周余华,彭方仁*，郝明灼,梁有旺,任莺，华宏. 低温胁迫下冬青叶片细胞内Ca²⁺水平及可溶性糖含量的变化[J]. 南京林业大学学报（自然科学版）, 2013, 37(05): 1-5.
[12]	叶青，魏建芬，解楠楠，姜琴，方伟. 自然低温胁迫对毛竹生理生化特性的影响应[J]. 南京林业大学学报（自然科学版）, 2011, 35(03): 27-.
[13]	王利民,陈金林*,梁珍海,赵好,王丽娜,薛丹,郭丽华. 盆栽滨梅幼苗对NaCl胁迫的响应[J]. 南京林业大学学报（自然科学版）, 2010, 34(03): 89-92.
[14]	薛建辉，苏敬，刘金根，吴永波. 5个常绿阔叶园林树种对低温变化的生理响应[J]. 南京林业大学学报（自然科学版）, 2009, 33(04): 38-42.
[15]	王相娥1，薛立1*，谢腾芳1，何跃君1,2. 低温处理对6种园林绿化树种幼苗生理生化特性的影响[J]. 南京林业大学学报（自然科学版）, 2009, 33(03): 139-142.