南京林业大学学报(自然科学版) ›› 2022, Vol. 46 ›› Issue (1): 122-130.doi: 10.12302/j.issn.1000-2006.202010026

• 研究论文 • 上一篇    下一篇

盐胁迫下AMF对榉树幼苗生长和光合特性的影响

马仕林1(), 曹鹏翔2, 张金池1,*(), 刘京1, 王金平1, 朱凌骏1, 袁钟鸣1   

  1. 1.南京林业大学林学院,南方现代林业协同创新中心,江苏 南京 210037
    2.江苏盐城大丰林场,江苏 盐城 224111
  • 收稿日期:2020-10-19 接受日期:2021-06-07 出版日期:2022-01-30 发布日期:2022-02-09
  • 通讯作者: 张金池
  • 基金资助:
    江苏省农业科技自主创新基金项目[CX(17)1004];国家林业公益性行业科研专项项目(201504406);林业科学技术推广项目([2015]17号);江苏高校优势学科建设工程资助项目(PAPD)

Effects of AMF on the growth and photosynthetic characteristics of Zelkova serrata under salt stress

MA Shilin1(), CAO Pengxiang2, ZHANG Jinchi1,*(), LIU Jing1, WANG Jinping1, ZHU Lingjun1, YUAN Zhongming1   

  1. 1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
    2. Dafeng Forest Farm, Yancheng 224111, China
  • Received:2020-10-19 Accepted:2021-06-07 Online:2022-01-30 Published:2022-02-09
  • Contact: ZHANG Jinchi

摘要:

【目的】探究盐胁迫下接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对榉树(Zelkova serrata)的生长和光合特性的影响以及光响应模型的适用性,为利用菌根真菌技术提高植物在盐碱地中的生产力提供理论依据。【方法】以榉树幼苗为试验材料,设置不接种AMF(N)、接种摩西球囊霉(Glomus mosseae)1号(GM1)、接种摩西球囊霉2号(GM2)、接种扭形球囊霉(G. tortuosum,GO)4个处理组,进行盐胁迫与非盐胁迫(CK)处理。通过对比分析不同处理间的差异,探索盐胁迫下AMF对榉树生长及光合特性的影响,并评定5种光响应模型的适用性。【结果】①盐胁迫下GM1、GO处理的菌根侵染率显著降低;②盐胁迫显著降低了榉树苗高、地径净增长量,而GM1、GM2处理显著提高了盐胁迫下榉树的地径净增长量;③盐胁迫下,榉树净光合速率明显下降,3种接菌处理均能提升榉树叶片净光合速率、气孔限制值、水分利用效率,并降低胞间CO2浓度,其中GM2和GO较未接菌处理变化最大;④盐胁迫条件下只有以叶子飘模型拟合的平均决定系数(R2)高于0.900,其余4种模型各处理的R2均低于0.900。【结论】盐胁迫对榉树幼苗生长及光合特性有抑制作用;接种AMF可以促进植物生长,提升植物净光合速率,缓解盐胁迫对植物生长及光合特性的抑制作用。综合AMF对榉树生长和光合特性的影响,GM2较为突出,为盐碱地林业生产推荐选用菌株;5种光响应模型中,叶子飘模型的拟合效果最好,为探究盐胁迫下AMF对榉树净光合速率影响的最优模型。

关键词: 盐胁迫, 榉树, 丛枝菌根真菌, 光合特性, 光响应模型

Abstract:

【Objective】 In recent years, the area of land salinization has been increasing with the impact of human activities and climate change. Excessive levels of soil salinity may lead to several ecological problems such as soil dispersion, soil salinization, desertification, destruction of vegetation communities, and reduction of biodiversity. As such, soil salinity is an important environmental factor limiting the continuing development of agriculture and forestry. As a crucial part of terrestrial ecosystems, arbuscular mycorrhizal fungi (AMF) play an important role in the management of saline-alkali land, while Zelkova serrata is a tree species with considerable economic and landscape value in China. Both species have wide application prospects in saline-alkali land forestry production. However, most existing research has focused on the physiological and biochemical characteristics and salt tolerance of Z. serrata, while few have investigated the effects of AMF on the photosynthetic characteristics of salt stressed Z. serrata. Moreover, there is a lack of research on the applicability of the light response model under this condition. Accurate characteristic parameters provided by a suitable light response model may directly or indirectly reflect the photosynthetic physiological process of plants, and offer considerable guidance for forestry production in saline-alkali land. As such, this study investigated the effects of arbuscular mycorrhizal fungi inoculation on the growth and photosynthetic characteristics of Z. serrata, and the applicability of light response model under salt stress. In summary, the objective was to provide a theoretical basis to use mycorrhizal combination technology to improve plant productivity in saline-alkali land.【Method】 Initially, Z. serrata seedlings that were not colonized with AMF were cultivated through seed and substrate sterilization. This was carried out concurrently while three AMF [i.e., Glomus mosseae separated from Bijie, Guizhou (GM1), G. mosseae separated from Chuxiong, Yunnan (GM2) and G. tortuosum separated from Ejin Horo Banner, Inner Mongolia (GO)], were multiplied in the artificial climate room for three months. Z. serrata seedlings with consistent growth were transplanted to the greenhouse of Nanjing Forestry University Xiashu Forestry Farm for the pot experiment and four inoculation treatments were established: (1) not inoculated with mycorrhizal fungi (N); (2) inoculated with GM1; (3) inoculated with GM2; and (4) inoculated with GO. After one year of AMF colonization and plant growth, potted Z. serrata seedlings of GM1, GM2, GO and N treatments were divided into two groups, and subjected to salt stress and non-salt stress treatments, respectively. In the salt stress treatment, 300 mL of 100 mmol/L NaCl solution was poured into each pot every week, and sterile distilled water was used to replace the NaCl solution in the non-salt stress treatment. The treatment was carried out for two months, during which the Hoagland (reduced phosphorus) nutrient solution was regularly replenished. Then, three pots were selected from each treatment to determine the photosynthetic characteristics and mycorrhizal colonization rate. The effect of AMF on the net height growth, net basal diameter growth, net photosynthetic rate, intercellular CO2 concentration, stomatal limit value, and water use efficiency of Z. serrata under salt stress was explored by comparing and analyzing differences between treatments. The applicability of five kinds of light response models was evaluated by plotting the light response curve and comparing the fitting accuracy of each model in each treatment. 【Result】 There were four key findings from the results. First, the mycorrhizal colonization rate of the GM1 and GO treatments decreased significantly under salt stress. Second, salt stress had a significant inhibitory impact on the net height growth and ground diameter growth of Z. serrata, while the GM1 and GM2 treatments significantly increased the net ground diameter growth under salt stress. Third, under salt stress, there was a clear decrease in the net photosynthetic rate of Z. serrata. All three treatments could increase the net photosynthetic rate, stomatal limit value, and water use efficiency, while reducing intercellular CO2 concentration. Among the treatments, the GM2 and GO treatments exhibited the largest changes compared with the N treatment. Fourth, only the average R2 fitted by the Ye Zi-Piao model was >0.900 under salt stress, and the R2 of the other four models were <0.900. 【Conclusion】 Salt stress was likely to inhibit plant growth and photosynthetic characteristics. Inoculation with AMF may promote plant growth, improve the net photosynthetic rate of plant leaves, and alleviate the inhibition of salt stress on plant growth and photosynthetic characteristics. Based on the influence of arbuscular mycorrhizal fungi inoculation on the growth and photosynthetic characteristics of Z. serrata, the GM2 was prominent; this was recommended as the strain for forestry production in saline-alkali land. Among the five light response models, the Ye Zi-Piao model exhibited the best fitting effect, representing the optimal model to explore the effect of AMF on the net photosynthetic rate of salt stressed Z. serrata.

Key words: salt stress, Zelkova serrata, arbuscular mycorrhizal fungi(AMF), photosynthetic characteristics, light response model

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