饲养林麝毛发皮质醇含量及其指示作用

doi:10.12302/j.issn.1000-2006.202103039

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (2): 236-240.doi: 10.12302/j.issn.1000-2006.202103039

饲养林麝毛发皮质醇含量及其指示作用

李大卫(), 胡德夫(), 张天祥, 郭小兵, 石明慧, 靳玮江, 张宝峰, 刘树强

北京林业大学生态与自然保护学院,北京 100083

收稿日期:2021-03-25 接受日期:2021-09-01 出版日期:2022-03-30 发布日期:2022-04-08
通讯作者: 胡德夫
基金资助:
国家自然科学基金项目(31872962)

Cortisol content in hair of captive forest musk deer(Moschus berezovskii) and its indication

LI Dawei(), HU Defu(), ZHANG Tianxiang, GUO Xiaobing, SHI Minghui, JIN Weijiang, ZHANG Baofeng, LIU Shuqiang

College of Ecology and Nature Conservation, Beijing Forestry University, Beijing 100083, China

Received:2021-03-25 Accepted:2021-09-01 Online:2022-03-30 Published:2022-04-08
Contact: HU Defu

摘要/Abstract

摘要：

【目的】探查饲养林麝的麝场规模、饲养空间及年龄因素对饲养林麝毛发生理应激的影响,为林麝饲养管理提供科学依据。【方法】采集陕西凤县25个麝场142只饲养雄性林麝同一部位的毛发,运用酶联免疫法(ELISA)测定林麝毛发皮质醇含量,比较不同年龄组林麝毛发皮质醇含量的差异,聚类分析林麝的毛发皮质醇含量,卡方检验高皮质醇组和低皮质醇组林麝在不同饲养环境中的分布情况,探查评估饲养雄性林麝的生理应激状态。【结果】饲养雄麝毛发皮质醇含量在3~9岁龄没有显著差异。毛发皮质醇含量的聚类分析表明,雄麝的生理应激状态可分为低皮质醇和高皮质醇2个组别;卡方检验结果显示,皮质醇的2个组别在不同规模麝场的分布不存在显著差异,在不同饲养空间中的分布存在显著差异,高皮质醇组林麝在小圈占比最高,低皮质醇组林麝在大圈占比最高。【结论】饲养条件下,根据林麝毛发皮质醇含量可有效探查其生理应激状态,可分为高敏感组和低敏感组;林麝饲养空间显著影响其生理状态,进而影响高敏感和低敏感个体的比例组成。

关键词: 林麝, 生理应激, 毛发皮质醇, 饲养环境

Abstract:

【Objective】We studied the effects of the size of the musk deer farm, enclosure space, and age factors on the physiological stress of forest musk deer (Moschus berezovskii) to provide a scientific basis for the feeding and management of the deer. 【Method】In this study, hair was taken from the left thigh of 142 captive male forest musk deer from 25 musk farms in Fengxian, Shaanxi, China. Enzyme-linked immunoassay (ELISA) was used to determine the cortisol content in the hair samples and the difference in the cortisol content in the hair of deer of different age groups. Additionally, the cluster analysis of the hair cortisol content of forest musk deer and a chi-square test of the distribution of the high cortisol group and the low cortisol group of forest musk deer in different feeding environments was used to explore and evaluate the physiological affecting farmed male forest musk deer.【Result】There was no significant difference in the cortisol content in the hair of male musk deer at the ages of 3-9 years. The cluster analysis of the hair cortisol content showed that the state of physiology stress in the male musk deer could be divided into two groups: low cortisol and high cortisol. The results of the chi-square test showed that there was no significant difference in the distribution of cortisol between the two groups at different scales of musk deer farms. The high cortisol group had the highest proportion in the small circle, and the low cortisol group had the highest proportion in the large circle.【Conclusion】The results showed that the cortisol content in forest musk deer hair could effectively detect the state of physiology, which could be divided into high-sensitivity and low-sensitivity groups. Feeding space significantly affected the physiological state of musk deer and affected the proportions of highly sensitive and less sensitive individuals.

Key words: forest musk deer(Moschus berezovskii), physiological stress, hair cortisol, feeding environment

中图分类号:

S864
S718

李大卫,胡德夫,张天祥,等. 饲养林麝毛发皮质醇含量及其指示作用[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 236-240.

LI Dawei, HU Defu, ZHANG Tianxiang, GUO Xiaobing, SHI Minghui, JIN Weijiang, ZHANG Baofeng, LIU Shuqiang. Cortisol content in hair of captive forest musk deer(Moschus berezovskii) and its indication[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(2): 236-240.DOI: 10.12302/j.issn.1000-2006.202103039.

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年龄组 age group	采样个体数 sampling individuals	毛发皮质醇含量/ (ng·g^-1) hair cortisol content
3岁组	39	476.66±159.42
4岁组	21	477.44±150.81
5岁组	15	486.26±238.76
6岁组	23	508.27±262.32
7岁组	25	449.90±154.72
8岁组	5	447.74±157.11
9岁组	14	499.09±239.14

分组 group	林麝样本数量 sample number of Moschus berezovskii	毛发皮质醇含量/ (ng·g^-1) hair cortisol content
Ⅰ	12	926.91±141.65
Ⅱ	130	438.08±135.14

养殖场规模 farm scale	分组 group	林麝数量 number of Moschus berezovskii	占比/% proportion
小规模 small scale	Ⅰ	10	10.2
小规模 small scale	Ⅱ	88	89.8
中规模 medium scale	Ⅰ	1	3.7
中规模 medium scale	Ⅱ	26	96.3
大规模 large scale	Ⅰ	1	5.9
大规模 large scale	Ⅱ	16	94.1

养殖场圈舍 farm enclosure	分组 group	林麝数量 number of Moschus berezovskii	占比/% proportion
小圈 small enclosure	Ⅰ	9	18.4
小圈 small enclosure	Ⅱ	40	81.6
中圈 middle enclosure	Ⅰ	2	4.3
中圈 middle enclosure	Ⅱ	44	95.7
大圈 large enclosure	Ⅰ	1	2.1
大圈 large enclosure	Ⅱ	46	97.9

饲养林麝毛发皮质醇含量及其指示作用

Cortisol content in hair of captive forest musk deer(Moschus berezovskii) and its indication

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