黄山马尾松林天牛及携带线虫种类初步调查

doi:10.12302/j.issn.1000-2006.202106041

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (4): 29-35.doi: 10.12302/j.issn.1000-2006.202106041

所属专题：松材线虫病致病机理与病害防治

• 专题报道Ⅰ：松材线虫病致病机理与病害防治（执行主编　叶建仁） • 上一篇下一篇

黄山马尾松林天牛及携带线虫种类初步调查

王立超¹(), 苏胜荣²(), 陈凤毛¹^,^*(), 董晓燕¹, 田成连³, 王洋¹

1.南京林业大学林学院,南方现代林业协同创新中心,江苏南京 210037
2.黄山学院生命与环境科学学院,安徽黄山 245041
3.黄山市林业局,安徽黄山 245021

收稿日期:2021-06-29 修回日期:2021-10-10 出版日期:2022-07-30 发布日期:2022-08-01
通讯作者: 陈凤毛
基金资助:
国家重点研发计划(2017YFD0600104);黄山学院人才启动项目(2021xkjq009)

Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City

WANG Lichao¹(), SU Shengrong²(), CHEN Fengmao¹^,^*(), DONG Xiaoyan¹, TIAN Chenglian³, WANG Yang¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. College of Life and Environmental Sciences, Huangshan University, Huangshan 245041, China
3. Huangshan City Forestry Bureau of Auhui Province, Huangshan 245021, China

Received:2021-06-29 Revised:2021-10-10 Online:2022-07-30 Published:2022-08-01
Contact: CHEN Fengmao

摘要/Abstract

摘要：

【目的】松材线虫病是我国危害最大的森林病害,松树一旦感病便迅速死亡。调查黄山地区马尾松林内天牛种类以及天牛携带线虫的变化规律,为黄山市松材线虫病的防控工作提供理论支持。【方法】在安徽省黄山市博村林场通过野外和室内收集天牛,并对天牛进行整姿、鉴定,记录天牛种类和数量。为确定可以携带线虫的媒介天牛,使用贝尔曼漏斗法对可以危害松树且有活力的天牛进行单头天牛线虫分离实验,12 h后,用15 mL离心管接取漏斗下端的天牛浸泡液,将浸泡液放在显微镜下观察是否含有线虫。根据携带线虫的天牛数量占天牛总数的比例计算天牛总体携带线虫比例;为鉴定天牛携带线虫种类,先通过室内真菌培养、观察线虫形态学特征对线虫进行初步鉴定,再使用松材线虫分子检测技术确定天牛携带的线虫是否为松材线虫,对于其他寄生线虫,通过28S rDNA基因进行扩增、测序、比对以及构建系统进化树进行分类鉴定。此外,统计不同时间携带线虫的天牛数量以及天牛携带线虫数量的变化。【结果】在黄山市博村林场共收集到4个亚科12个属的14种天牛,通过线虫分离实验确定可以携带线虫的天牛主要有2种:松墨天牛(Monochamus alternatus)和小灰长角天牛(Acanthocinus griseus),2种天牛整体线虫携带率分别为45.8%和13.6%,通过形态学观察松墨天牛和小灰长角天牛均可携带两种不同形态的扩散型线虫,一种是尖尾形线虫,另一种则是圆尾形线虫。尖尾形线虫通过形态学和松材线虫分子检测鉴定被确定为松材线虫(Bursaphelenchus xylophilus),而圆尾形线虫则通过形态学和系统发育树结果被确定为Contortylenchus sp.类线虫。6月19日至7月23日每周线虫分离试验结果表明,松墨天牛雌成虫携带线虫的比例依次为76.5%、66.0%、52.9%、34.7%和10.0%;松墨天牛雄成虫携带线虫的比例依次为76.5%、60.0%、50.0%、30.8%和14.7%,松墨天牛雌成虫携带线虫的数量依次为1 018.0、530.1、202.5、200.4和80.0条/头,松墨天牛雄成虫携带线虫的数量依次为495.1、68.3、74.9、8.2和3.8条/头。【结论】黄山市博村林场马尾松林内共收集到14种天牛,其中松墨天牛和小灰长角天牛都可以携带松材线虫和Contortylenchus sp.类线虫,但以松墨天牛为主,松墨天牛携带线虫的数量和比例随携带时间推移逐渐减少。

关键词: 松材线虫, 媒介昆虫, 松墨天牛, 小灰长角天牛, 马尾松

Abstract:

【Objective】The pine wilt disease is one of the serious forestry diseases in China, and causes rapid death in pine trees. To provide a theoretical support for the prevention and control of pine wood disease in Huangshan City, an investigation on the species of longicorn beetles and related nematodes was carried out in Pinus massoniana forest. 【Method】Longicorn beetles were collected indoors and outdoors from the Bocun forest farm. The beetles were fixed and identified, and their species and number were recorded. To confirm the presence of nematode vectors, Baermann funnel technique was used to extract the nematodes from beetles, which are harmful to the vitality of pine trees. After soaking the beetles for 12 h, a 15 mL centrifuge tube was used to place the soaking solution at the end of the funnel, which was then observed under a microscope to determine the presence or absence of nematodes. The ratio of beetles that carry nematodes was calculated by determining the ratio of nematode-containing beetles to the total number. To identify the species of nematodes carried by beetles, they were first cultured with fungi and preliminarily identified by observing their morphological characteristics. The molecular identification technique of Bursaphelenchus xylophilus was used to determine if the nematode was B. xylophilus. For the other parasitic nematodes, the 28S rDNA gene of nematodes was amplified, sequenced, blasted, and the phylogenetic tree was constructed for identification. Besides, the ratio and the number of nematodes carried by beetles at different times were counted. 【Result】 Total 14 longicorn beetles belonging to 12 genera and four subfamilies were collected. Two species of beetles Monochamus alternatus and Acanthocinus griseus were associated with nematodes, and their overall nematode carrying rates were 45.8% and 13.6%, respectively. M. alternatus and A. griseus both carry two types of dispersal nematodes with different morphological characteristics, one with a pointed tail and the other with a rounded tail. Results of the morphological and molecular analysis revealed the nematode with the pointed tail as B. xylophilus, while the other as Contortylenchus sp. During the period from June 19 to July 23, the weekly nematode isolation test results showed that the nematodes carried by female adults of M. alternatus were 76.5%, 66.0%, 52.9%, 34.7% and 10.0%, respectively; the proportion of nematodes carried by male adults of M. alternatus was 76.5%, 60.0%, 50.0%, 30.8% and 14.7%. The average number of nematodes carried by per individual female adult of M. alternatus was 1 018.0, 530.1, 202.5, 200.4 and 80.0, and the average number of nematodes carried by per individual male adult was 495.1, 68.3, 74.9, 8.2 and 3.8. 【Conclusion】 A total of 14 longicorn beetles were recorded in the masson pine forest in Bocun forest farm, Huangshan City. Among them, M. alternatus and A. griseus can carry B. xylophilus and Convertylenchus sp., the former being the main strain. The number and proportion of M. alternatus carrying nematodes gradually decreased with time.

Key words: Bursaphelenchus xylophilus, vector insect, Monochamus alternatus, Acanthocinus griseus, Pinus massoniana

中图分类号:

S763

王立超,苏胜荣,陈凤毛,等. 黄山马尾松林天牛及携带线虫种类初步调查[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 29-35.

WANG Lichao, SU Shengrong, CHEN Fengmao, DONG Xiaoyan, TIAN Chenglian, WANG Yang. Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(4): 29-35.DOI: 10.12302/j.issn.1000-2006.202106041.

图/表 5

表1

图1

图2

图3

图4

参考文献 42

[1]	FUTAI K. Pine wood nematode,Bursaphelenchus xylophilus[J]. Annu Rev Phytopathol, 2013, 51:61-83.DOI:10.1146/annurev-phyto-081211-172910.
[2]	程瑚瑞, 林茂松, 黎伟强, 等. 南京黑松上发生的萎蔫线虫病[J]. 森林病虫通讯, 1983, 2(4):1-5.
	CHENG H R, LIN M S, LI W Q, et al. Pine wilt disease on Pinus thunbergia in Nanjing[J]. For Pest Dis, 1983, 2(4):1-5.
[3]	叶建仁. 松材线虫病在中国的流行现状、防治技术与对策分析[J]. 林业科学, 2019, 55(9):1-10.
	YE J R. Epidemic status of pine wilt disease in China and its prevention and control techniques and counter measures[J]. Sci Silvae Sin, 2019, 55(9):1-10.DOI:10.11707/j.1001-7488.20190901.
[4]	陈婷婷, 叶建仁, 吴小芹, 等. 抗松材线虫病马尾松体胚发生与植株再生条件的优化[J]. 南京林业大学学报(自然科学版), 2019, 43(3):1-8.
	CHEN T T, YE J R, WU X Q, et al. Somatic embryogenesis and plantlet regeneration of disease-resistant Pinus massoniana Lamb[J]. J Nanjing For Univ (Nat Sci Ed),2019, 43(3):1-8.DOI:10.3969/j.issn.1000-2006.201806005.
[5]	尹艳楠, 谈家金, 李梦伟, 等. 蜡样芽孢杆菌NJSZ-13菌株防治松材线虫病研究[J]. 南京林业大学学报(自然科学版), 2021, 45(3):152-158.
	YIN Y N, TAN J J, LI M W, et al. A study on the biocontrol of pine wilt disease by Bacillus cereus NJSZ-13[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(3):152-158.DOI: 10.12302/j.issn.1000-2006.201910013.
[6]	潘佳亮, 姚翰文, 董瀛谦, 等. 2019年全国松材线虫病疫情分析[J]. 中国森林病虫, 2021, 40(1):32-37.
	PAN J L, YAO H W, DONG Y Q, et al. Analysis of the epidemic situation of pine wilt disease in China in 2019[J]. For Pest Dis, 2021, 40(1):32-37.DOI:10.19688/j.cnki.issn1671-0886.20200042.
[7]	国家林业局. 国家林业局2017年第4号公告[EB/OL]. (2017-01-11)[2021-06-30]. http://www.forestry.gov.cn/sites/main/main/gov/content.jsp?TID=2344.
[8]	于海英, 吴昊, 张旭东, 等. 落叶松自然条件下感染松材线虫初报[J]. 中国森林病虫, 2019, 38(4):7-10.
	YU H Y, WU H, ZHANG X D, et al. Preliminary study on Larix spp.infected by Bursaphelenchus xylophilus in natural environment[J]. For Pest Dis, 2019, 38(4):7-10.DOI:10.19688/j.cnki.issn1671-0886.20180024.
[9]	国家林业和草原局. 国家林业和草原局2019年第20号公告[EB/OL]. (2019-12-12) [2021-06-30]. http://www.gov.cn/xinwen/2019-12/18/content_5462013.htm.
[10]	丁晓磊, 张悦, 林司曦, 等. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报(自然科学版), 2022, 46(4):1-7.
	DING X L, ZHANG Y, LIN S X, et al. An overview of high-throughput sequencing techniques applied on Bursaphelenchus xylophilus[J]. J Nanjing For Univ(Nat Sci Ed): 2022, 46(4):1-7.DOI:10.12302/j.issn.1000-2006.202204039.
[11]	郑凡. 黄山市森林资源现状及变化分析[J]. 安徽林业科技, 2015, 41(3):54-57.
	ZHENG F. Analysis on forest resource actualities and changes of Huangshan City[J]. Anhui For Sci Technol, 2015, 41(3):54-57.DOI:10.3969/j.issn.2095-0152.2015.03.016.
[12]	国家林业和草原局. 国家林业局2018年第1号公告[EB/OL]. (2018-02-02)[2021-06-30]. http://www.forestry.gov.cn/sites/main/main/gov/content.jsp?TID=2465.
[13]	国家林业和草原局. 国家林草局2019年第4号公告[EB/OL]. (2019-01-23)[2021-06-30]. http://www.forestry.gov.cn/zlszz/5356/20190517/054027583884147.html.
[14]	MAMIYA Y, ENDA N. Transmission of Bursaphelenchus lignicolus (Nematoda:Aphelenchoididae) by Monochamus alternatus (Coleoptera:Cerambycidae)[J]. Nematologica, 1972, 18(2):159-162.DOI:10.1163/187529272x00395.
[15]	WINGFIELD M J, BLANCHETTE R A. The pine-wood nematode,Bursaphelenchus xylophilus,in Minnesota and Wisconsin:insect associates and transmission studies[J]. Can J For Res, 1983, 13(6):1068-1076.DOI:10.1139/x83-143.
[16]	LINIT M J, KONDO E, SMITH M T. Insects associated with the pinewood nematode,Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae),in Missouri[J]. Environ Entomol, 1983, 12(2):467-470.DOI:10.1093/ee/12.2.467.
[17]	SOUSA E, NAVES P, BONIFÁCIO L, et al. Preliminary survey for insects associated with Bursaphelenchus xylophilus in Portugal[J]. EPPO Bull, 2002, 32(3):499-502.DOI:10.1046/j.1365-2338.2002.00597.x.
[18]	徐福元, 杨宝君, 葛明宏. 松材线虫病媒介昆虫的调查[J]. 森林病虫通讯, 1993, 12(2):20-21.
	XU F Y, YANG B J, GE M H. Investigation on insects associated with Bursaphelenchus xylophilus[J]. For Pest Dis, 1993, 12(2):20-21.
[19]	WANG Y, CHEN F M, WANG L C, et al. Investigation of beetle species that carry the pine wood nematode,Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle,in China[J]. J For Res, 2021, 32(4):1745-1751.DOI:10.1007/s11676-020-01146-2.
[20]	LI M, LI H, SHENG R C, et al. The first record of Monochamus saltuarius (Coleoptera;Cerambycidae) as vector of Bursaphelenchus xylophilus and its new potential hosts in China[J]. Insects, 2020, 11(9):636.DOI:10.3390/insects11090636.
[21]	王玉嬿, 石运琳, 周新胜, 等. 南京地区黑松萎蔫线虫病发病规律的初步观察[J]. 森林病虫通讯, 1985, 4(2):15-17.
	WANG Y Y, SHI Y L, ZHOU X S, et al. Preliminary observation on the pathogenesis of pine wilt disease of Pinus thunbergii in Nanjing[J]. For Pest Dis, 1985, 4(2):15-17.
[22]	赵锦年, 余盛明, 王浩杰, 等. 黄山风景区松蛀虫及携带线虫潜能的研究[J]. 中国森林病虫, 2004, 23(4):15-18.
	ZHAO J N, YU S M, WANG H J, et al. Pine borers in Huangshan Scenic Area and the potential for carrying nematodes by them[J]. For Pest Dis, 2004, 23(4):15-18.DOI:10.3969/j.issn.1671-0886.2004.04.005.
[23]	KANZAKI N, MAEHARA N, AKIBA M, et al. Morphological characters of dauer juveniles of three species of Bursaphelenchus Fuchs,1937[J]. Nematology, 2016, 18(2):209-220.DOI:10.1163/15685411-00002954.
[24]	陈凤毛, 叶建仁, 吴小芹. 松材线虫实时PCR检测技术[J]. 南京林业大学学报(自然科学版), 2007, 31(4):121-124.
	CHEN F M, YE J R, WU X Q. Detection technique of Bursaphelenchus xylophilus using real time PCR[J]. J Nanjing For Univ (Nat Sci Ed),2007, 31(4):121-124.DOI:10.3969/j.issn.1000-2006.2007.04.027.
[25]	陈凤毛, 叶建仁, 吴小芹, 等. 松材线虫两种实用分子检测技术[J]. 北京林业大学学报, 2011, 33(4):149-152.
	CHEN F M, YE J R, WU X Q, et al. Two kinds of applied molecular skills to detect Bursaphelenchus xylophilus[J]. J Beijing For Univ, 2011, 33(4):149-152.DOI:10.13332/j.1000-1522.2011.04.012.
[26]	宋雅婷, 王立超, 孙守慧, 等. 滑刃属线虫1个中国新记录种[J]. 南京林业大学学报(自然科学版), 2020, 44(3):105-110.
	SONG Y T, WANG L C, SUN S H, et al. First report of Aphelenchoides rotundicaudatus in China[J]. J Nanjing For Univ (Nat Sci Ed),2020, 44(3):105-110.DOI:10.3969/j.issn.1000-2006.201909046.
[27]	黄任娥, 叶建仁, 潘宏阳, 等. 感染松材线虫病松树滑刃目线虫调查[J]. 植物病理学报, 2008, 38(2):136-146.
	HUANG R E, YE J R, PAN H Y, et al. Investigation on Aphelenchida(Nematoda) from pine wood infected with Bursaphelenchus xylophilus[J]. Acta Phytopathol Sin, 2008, 38(2):136-146.DOI:10.13926/j.cnki.apps.2008.02.018.
[28]	史延梅, 陈凤毛, 叶建仁, 等. 我国松树寄生线虫分类检索[J]. 南京林业大学学报(自然科学版), 2012, 36(1):137-141.
	SHI Y M, CHEN F M, YE J R, et al. Classification of parasitic Nematodes in pine trees in China[J]. J Nanjing For Univ (Nat Sci Ed), 2012, 36(1):137-141.DOI:10.3969/j.issn.1000-2006.2012.01.028.
[29]	汤坚, 叶建仁, 陈凤毛, 等. 松树体内寄生线虫种类及其致病性[J]. 南京林业大学学报(自然科学版), 2008, 32(3):112-116.
	TANG J, YE J R, CHEN F M, et al. Species of parasitic nematode in pine host and pathogenicity[J]. J Nanjing For Univ (Nat Sci Ed),2008, 32(3):112-116.DOI:10.3969/j.issn.1000-2006.2008.03.027.
[30]	谢立群, 巨云为, 赵博光. 松材线虫传播机理的研究进展[J]. 安徽农业科学, 2007, 35(19):5798-5800,5867.
	XIE L Q, JU Y W, ZHAO B G. Review on transmission mechanism of Bursaphelenchus xylophilus[J]. J Anhui Agric Sci, 2007, 35(19):5798-5800,5867.DOI:10.13989/j.cnki.0517-6611.2007.19.075.
[31]	FUKUSHIGE H. The number of Bursaphelenchus xylophilus carried by Monochamus alternatus and some possible factors regulating the number[J]. Jpn J Nematol, 1990, 20:18-24.
[32]	MAEHARA N, FUTAI K. Factors affecting the number of Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae) carried by several species of beetles[J]. Nematology, 2002, 4(5):653-658.DOI:10.1163/15685410260438944.
[33]	TOGASHI K. Factors affecting the number of Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae) carried by newly emerged adults of Monochamus alternatus (Coleoptera:Cerambycidae)[J]. Appl Entomol Zool, 1989, 24(4):379-386.DOI:10.1303/aez.24.379.
[34]	JIKUMARU S, TOGASHI K. Inhibitory effect of Bursaphelenchus mucronatus (nematoda:Aphelenchoididae) on B.xylophilus boarding adult Monochamus alternatus (coleoptera:Cerambycidae)[J]. J Nematol, 2004, 36(1):95-99.
[35]	MAEHARA N, TSUDA K, YAMASAKI M, et al. Effect of fungus inoculation on the number of Bursaphelenchus xylophilus (Nematoda:Aphelenchoididae) carried by Monochamus alternatus (Coleoptera:Cerambycidae)[J]. Nematology, 2006, 8(1):59-67.DOI:10.1163/156854106776179944.
[36]	田成连. 黄山市松墨天牛及小灰长角天牛羽化规律研究[J]. 安徽林业科技, 2018, 44(5):8-9,13.
	TIAN C L. Study on the eclosion regularities of Monochamus alternatus and Acanthocinus griseus in Huangshan City[J]. Anhui For Sci Technol, 2018, 44(5):8-9,13.DOI:10.3969/j.issn.2095-0152.2018.05.003.
[37]	来燕学, 杨忠岐, 王小艺, 等. 卵巢线虫(Contortylenchus genitalicola)在中国松褐天牛上的分布与作用研究[J]. 林业科学研究, 2018, 31(1):51-59.
	LAI Y X, YANG Z Q, WANG X Y, et al. Distribution and function of the ovarian nematode Contortylenchus genitalicola(Tylenchida:Allantonematidae) on Monochamus alternatus(Coleoptera:Cerambycidae) in China[J]. For Res, 2018, 31(1):51-59.DOI:10.13275/j.cnki.lykxyj.2018.01.006.
[38]	KOSAKA H, OGURA N. Biology of a tylenchid nematode parasitic on the Japanese pine sawyer,Monochamus alternatus[J]. Nematologica, 1991, 37(1/2/3/4):455-469.DOI:10.1163/187529291x00448.
[39]	KOSAKA H, OGURA N. Contortylenchus genitalicola n.sp.(Tylenchida:Allantonematidae)from the Japanese pine sawyer,Monochamus alternatus(Coleoptera:Cerambycidae)[J]. Appl Entomol Zool, 1993, 28(4):423-432.DOI:10.1303/aez.28.423.
[40]	陈敬祥, 程杰, 林同. 松墨天牛dynamin-1-like protein基因的鉴定及表达分析[J]. 江苏农业学报, 2017(3):524-532.
	CHEN J X, CHENG J, LIN T. Identification and expression of dynamin-1-like protein gene from Monochamus alternatus[J]. Jiangsu J Agric Sci, 2017(3):524-532.DOI:10.3969/j.issn.1000-4440.2017.03.007.
[41]	赵锦年, 张常青, 戴建昌, 等. 松墨天牛成虫羽化逸出及其携带松材线虫能力的研究[J]. 林业科学研究, 1999, 12(6):572-576.
	ZHAO J N, ZHANG C Q, DAI J C, et al. Studies of emerge emigration of Monochamus alternatus and its ability of carrying nematode[J]. For Res, 1999, 12(6):572-576.DOI:10.3321/j.issn:1001-1498.1999.06.002.
[42]	柴希民, 张都海, 张国贤, 等. 松褐天牛成虫携带松材线虫的数量[J]. 东北林业大学学报, 2000, 28(5):99-101.
	CHAI X M, ZHANG D H, ZHANG G X, et al. The number of Bursaphelenchus xylophilus carried by Monochamus alternatus adults[J]. J Northeast For Univ, 2000, 28(5):99-101.DOI:10.13759/j.cnki.dlxb.2000.05.023.

诱捕天牛种类 species of longicorn beetles	数量/头 number of longicorn beetle trapped
松墨天牛 Monochamus alternatus	5 910
小灰长角天牛 Acanthocinus griseus	22
短角幽天牛 Spondylis buprestoides	3
赤梗天牛 Arhopalus unicalor	3
桃红颈天牛 Aromia bungii	2
星天牛 Anoplophora chinensis	1
八星粉天牛 Olenecamptus octopustulatus	1
弧纹虎天牛 Chlorophorus miwai	18
黄荆重突天牛 Tetraophthalmus episcopalis	1
桔狭胸天牛 Philus antennatus	2
斜顶天牛 Pseudoterinaea bicoloripes	1
斑角坡天牛 Pterolophia annulata	14
瘤胸簇天牛 Aristobia hispida	1
齿角坡天牛 Pterolophia serrata	1

黄山马尾松林天牛及携带线虫种类初步调查

Preliminary investigations of vector beetle and nematode species in Pinus massoniana forest in Huangshan City

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 42

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	范明阳, 胡萌, 杨园, 方炎明. 中国东部地区马尾松与黄山松群落分类及群落结构和物种多样性特征[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 47-58.
[2]	王章荣, 季孔庶, 徐立安, 邹秉章, 林能庆, 林景泉. 马尾松实生种子园营建技术、现实增益及多世代低成本经营新模式探讨[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 9-16.
[3]	王宇, 易艳灵, 刘海, 文晓晨, 李天一, 尹海锋, 李贤伟, 范川. 两种采伐方式对马尾松人工林林分空间结构的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 138-146.
[4]	王立超, 陈凤毛, 董晓燕, 田成连, 王洋. 松墨天牛取食和产卵特性研究[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 219-224.
[5]	竹磊, 徐军亮, 章异平, 罗鹏飞, 师志强, 候佳玉, 翟乐鑫. 河南洛阳马尾松树干液流昼夜变化特征及其影响因子分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 92-100.
[6]	孙薇, 王斌, 楚秀丽, 王秀花, 张东北, 吴小林, 周志春. 马尾松容器苗生长和养分性状对磷添加和接种菌根菌的响应及关联[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 226-233.
[7]	季孔庶, 徐立安, 王登宝, 倪州献, 王章荣. 中国马尾松遗传改良研究历程与成就[J]. 南京林业大学学报（自然科学版）, 2022, 46(6): 10-22.
[8]	丁晓磊, 张悦, 林司曦, 叶建仁. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 1-7.
[9]	韦鹏飞, 理永霞, 冯宇倩, 刘振凯, 张星耀. 松材线虫性别决定基因Bx-sex-1表达特征和生物学功能分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 15-20.
[10]	汪青桐, 丁晓磊, 叶建仁, 史秀峰. 基于SNP分子标记的华东地区松材线虫种群遗传分化研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 21-28.
[11]	王磊, 叶建仁, 史丽娜. 利用腐生线虫加速替代疫木中松材线虫种群数量研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 36-44.
[12]	江奕, 陈凤毛. 喜旱莲子草与乌蔹莓等植物提取物对松材线虫的毒杀活性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 45-52.
[13]	吴佳雯, 尹艳楠, 谈家金, 郝德君. 蜡样芽孢杆菌NJSZ-13菌株诱导马尾松抗松材线虫病研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 53-58.
[14]	张瑞芝, 姜生伟, 吴昊, 陈俏丽, 李丹蕾, 王峰. 松材线虫Bx-HSF-1转录激活活性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 8-14.
[15]	胡兴峰, 吴帆, 孙晓波, 陈厚平, 殷安政, 季孔庶. 38年生马尾松种源生长及材性联合分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 203-212.