印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

doi:10.12302/j.issn.1000-2006.202203014

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2): 101-106.doi: 10.12302/j.issn.1000-2006.202203014

印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

母洪娜¹^,²(), 王炜², 樊蕾², 吴楚², 郭晓华², 孙陶泽¹^,²^,^*()

1.长江大学,湿地生态与农业利用教育部工程研究中心,湖北荆州 434025
2.长江大学园艺园林学院,湖北荆州 434025

收稿日期:2022-03-04 修回日期:2022-09-22 出版日期:2023-03-30 发布日期:2023-03-28
通讯作者: * 孙陶泽(suntaoze@yangtzeu.edu.cn),讲师。
基金资助:
湿地工程中心开放基金(KF201915);国家自然科学基金青年项目(51908063);湖北省教育厅哲学社会科学研究项目(21Y058);长江大学本科生“课程思政”项目(长江大学校发[2020]65号)

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

MU Hongna¹^,²(), WANG Wei², FAN Lei², WU Chu², GUO Xiaohua², SUN Taoze¹^,²^,^*()

1. Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China
2. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Received:2022-03-04 Revised:2022-09-22 Online:2023-03-30 Published:2023-03-28

摘要/Abstract

摘要：

【目的】研究了干旱胁迫下印度梨形孢(Piriformospora indica)对桂花(Osmanthus fragrans)的接种效应,为桂花节水低碳的栽培模式提供理论依据。【方法】以2年生桂花苗为材料,通过园土盆栽的方法,设置接种(40 mL含1 g新鲜印度梨形孢菌丝体的菌剂)+干旱(土壤含水量为田间最大持水量的20%~25%)、接种+轻度干旱(土壤含水量为田间最大持水量的45%~50%)、接种+正常供水(土壤含水量为田间最大持水量的65%~70%)、对照(40 mL灭活菌剂)+干旱、对照+轻度干旱、对照+正常供水共6个处理。【结果】印度梨形孢在桂花根系中定殖率高达81%。与对照组相比,接种印度梨形孢的桂花苗在轻度干旱和干旱胁迫下,株高、地径、干质量均高于对照,尤其是接种+干旱处理达到显著或极显著水平。与对照+干旱处理相比,接种后的桂花过氧化物酶(POD)与超氧化物歧化酶(SOD)、脯氨酸和可溶性糖含量分别增加了13.8%、37.9%、47.6%、40.7%。接种+干旱组的丙二醛含量比对照+干旱组增长量大幅降低。【结论】印度梨形孢与桂花共生能促进干旱(伴随夏季高温)胁迫下桂花生长,可能是其通过刺激SOD、POD活性增加和渗透调节物质的显著积累来抵御干旱胁迫;而轻度干旱和正常供水条件下,接种印度梨形孢对桂花生长无显著作用。

关键词: 印度梨形孢, 桂花, 干旱胁迫, 渗透调节, 抗旱性

Abstract:

【Objective】 The colonization effects of Piriformospora indica on Osmanthus fragrans under drought stress were analyzed. 【Method】 Two-year seedlings cultivated in pots with soil were used for this study. The experiment design was set up with the following parameters: inoculation (40 mL mycelium of P. indica) + drought (the soil water content is 20%-25% of the maximum field water capacity), inoculation + slight drought (45%-50% of the maximum field water capacity), inoculation + normal irrigation (65%-70% of the maximum field water capacity), control (40 mL inactivator) + drought, control + slight drought, and control + drought, six treatments in all. 【Result】 (1) Results showed that the colonization rate of P. indica was up to 81%. (2) Compared to the controls, the inoculated seedlings presented a better performance in plant height, stem diameter, and dry mass under both slight drought and drought stresses, especially the inoculation + drought groups, which showed significant or even extremely significant differences. (3) The protective enzyme activity of POD and SOD and the contents of proline and soluble sugar increased by 13.8%, 37.9%, 47.6% and 40.7%, respectively. (4) In addition, the MDA content decreased more quickly in the inoculation group than in the control group (control + drought). 【Conclusion】 This study found that the symbiosis of P. indica with O. fragrans played a positive role for O. fragrans seedlings in withstanding the negative effects of drought stress summer, most likely by stimulating the increase of SOD and POD enzyme activities and the significant accumulation of osmoregulatory substances. In contrast, inoculation with P. indica had no significant effect on the growth of O. fragrans under mild drought and normal water supply conditions.

Key words: Piriformospora indica, Osmanthus fragrans, drought stress, osmotic adjustment, drought resistance

中图分类号:

S685.13

母洪娜,王炜,樊蕾,等. 印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 101-106.

MU Hongna, WANG Wei, FAN Lei, WU Chu, GUO Xiaohua, SUN Taoze. Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(2): 101-106.DOI: 10.12302/j.issn.1000-2006.202203014.

图/表 5

图1

表1

图2

图3

图4

参考文献 26

[1]	母洪娜, 王良桂, 孙陶泽. 桂花多酚氧化酶 (PPO)基因的克隆及表达分析[J]. 分子植物育种, 2017, 15(2): 441-446.
	MU H N, WANG L G, SUN T Z. Cloning and expression analysis of PPO gene from sweet Osmanthus(Osmanthus fragrans Lour.)[J], Mol Plant Breed, 2017, 15(2): 441-446. DOI: 10.13271/j.mpb.015.000441.
[2]	WANG L, TAN N N, HU J Y, et al. Analysis of the main active ingredients and bioactivities of essential oil from Osmanthus fragrans var. thunbergii using a complex network approach[J]. BMC Syst Biol, 2017, 11(1): 144. DOI: 10.1186/s12918-017-0523-0.
[3]	XIONG L N, MAO S Q, LU B Y, et al. Osmanthus fragrans flower extract and acteoside protect against d-galactose-induced aging in an ICR mouse model[J]. J Med Food, 2016, 19(1): 54-61. DOI: 10.1089/jmf.2015.3462.
[4]	MAR A, PRIPDEEVECH P. Volatile components of crude extracts of Osmanthus fragrans flowers and their antibacterial and antifungal activities[J]. Chem Nat Compd, 2016, 52(6): 1106-1109. DOI: 10.1007/s10600-016-1876-0.
[5]	尹伟, 郁阳, 马秋丽, 等. 桂花叶的化学成分及抗肿瘤活性研究[J]. 热带亚热带植物学报, 2018, 26(2): 178-184.
	YIN W, YU Y, MA Q L, et al. Study on chemical constituents and antitumor activities of leaves of Osmanthus fragrans[J]. J Trop Subtrop Bot, 2018, 26(2): 178-184. DOI: 10.11926/jtsb.3786.
[6]	崔朋飞, 朱先奇, 李玮. 中国农业碳排放的动态演进与影响因素分析[J]. 世界农业, 2018(4): 127-134.
	CUI P F, ZHU X Q, LI W. Dynamic evolution and influencing factors of agricultural carbon emissions in China[J]. World Agric, 2018(4): 127-134. DOI: 10.13856/j.cn11-1097/s.2018.04.019.
[7]	姜华, 毕玉芬, 陈连仙, 等. 旱作条件下紫花苜蓿生理特性的研究[J]. 草地学报, 2012, 20(6): 1077-1080.
	JIANG H, BI Y F, CHEN L X, et al. Physiological characteristics of alfalfa under dry-farming conditions[J]. Acta Agrestia Sin, 2012, 20(6): 1077-1080. DOI: 10.11733/j.issn.1007-0435.2012.06.015.
[8]	VERMA S, VARMA A, REXER K H, et al. Piriformospora indica, Gen. et sp. nov., a new root-colonizing fungus[J]. Mycologia, 1998, 90(5): 896-903. DOI: 10.2307/3761331.
[9]	SUN C, JOHNSONA J M, CAI D G, et al. Piriformospora indica confers drought tolerance in Chinese cabbage leaves by stimulating antioxidant enzymes, the expression of drought-related genes and the plastid-localized CAS protein[J]. J Plant Physiol, 2010, 167(12): 1009-1017. DOI: 10.1016/j.jplph.2010.02.013.
[10]	WALLER F, ACHATZ B, BALTRUSCHAT H, et al. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance and higher yield[J]. Proc Natl Acad Sci USA, 2005, 102(38): 13386-13391. DOI: 10.1073/pnas.0504423102.
[11]	GHABOOLI M, KHATABI B, AHMADI F S, et al. Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley[J]. J Proteom, 2013, 94: 289-301. DOI: 10.1016/j.jprot.2013.09.017.
[12]	武美燕, 蒿若超, 张文英. 印度梨形孢真菌对干旱胁迫下紫花苜蓿生长及抗旱性的影响[J]. 草业学报, 2016, 25(5): 78-86.
	WU M Y, HAO R C, ZHANG W Y. Effects of Piriformospora indica fungus on growth and drought resistance in alfalfa under water deficit stress[J]. Acta Prataculturae Sin, 2016, 25(5): 78-86. DOI: 10.11686/cyxb2015366.
[13]	主朋月, 韩冰, 王晓阳, 等. 印度梨形孢联合紫花苜蓿修复土壤镉污染研究[J]. 环境科学与技术, 2019, 42(6): 21-27.
	ZHU P Y, HAN B, WANG X Y, et al. Study on the remediation of cadmium pollution in soil by combination of Medicago sativa and Piriformospora indica[J]. Environ Sci & Technol, 2019, 42(6): 21-27. DOI: 10.19672/j.cnki.1003-6504.2019.06.003.
[14]	杨芮. 印度梨形孢诱导红掌抗干旱和低温胁迫响应[D]. 福州: 福建农林大学, 2018.
	YANG R. In response to drought and low temperature stress induced by piriformospora indica[D]. Fuzhou: Fujian Agriculture and Forestry University, 2018.
[15]	袁芳. 印度梨形孢提高毛竹耐盐能力及其机理的初步研究[D]. 杭州: 浙江农林大学, 2019.
	YUAN F. Preliminary study on improving the salt tolerance of Phyllostachys edulis by Piriformospora indica and its mechanisms[D]. Hangzhou: Zhejiang A & F University, 2019.
[16]	PHILLIPS J M, HAYMAN D S. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J]. Trans Br Mycol Soc, 1970, 55(1): 158-IN18. DOI: 10.1016/S0007-1536(70)80110-3.
[17]	苍晶, 赵会杰. 植物生理学实验教程[M]. 北京: 高等教育出版社, 2013.
	CANG J, ZHAO H J. Experimental course of plant physiology[M]. Beijing: Higher Education Press, 2013.
[18]	韦巧, 武美燕, 张文英. 不同磷水平下印度梨形孢对生菜生长及磷素吸收利用的影响[J]. 河南农业科学, 2017, 46(1): 100-104.
	WEI Q, WU M Y, ZHANG W Y. Effects of Piriformospora indica on growth and phosphorus absoption of lettuce under different phosphorus levels[J]. J Henan Agric Sci, 2017, 46(1): 100-104. DOI: 10.15933/j.cnki.1004-3268.2017.01.018.
[19]	邢鸿林, 刘天义, 扎史都吉, 等. 光照与氮添加对红皮云杉幼树生长与叶形态功能的影响[J]. 森林工程, 2022, 38(4): 1-9.
	XING H L, LIU T Y, Zhashi Duji, et al. Effects of light and nitrogen addition on the tree growth, needle morphological and functions of Picea koraiensis saplings[J]. Forest Engineering, 2022, 38(4): 1-9.
[20]	魏媛, 张金池, 尹晓阳, 等. 华山松菌根化幼苗的抗旱特性[J]. 南京林业大学学报(自然科学版), 2007, 31(4):69-72.
	WEI Y, ZHANG J C, YIN X Y, et al. Characteristics of drought tolerance in mycorrhizal fungi seedlings of Pinus armandii franch[J]. J Nanjing For Univ (Nat Sci Ed), 2007, 31(4):69-72. DOI: 10.3969/j.issn.1000-2006.2007.04.015.
[21]	赵金梅, 周禾, 王秀艳. 水分胁迫下苜蓿品种抗旱生理生化指标变化及其相互关系[J]. 草地学报, 2005, 13(3): 184-189.
	ZHAO J M, ZHOU H, WANG X Y. Effect of water stress on physiological and biochemical process of alfalfa varieties[J]. Acta Agrestia Sin, 2005, 13(3): 184-189. DOI: 10.11733/j.issn.1007-0435.2005.03.003.
[22]	关追追, 赵江宁, 邱权, 等. 楸树人工林生长规律及其最优生长模型研究: 以河南省洛宁县楸树为例[J]. 森林工程, 2021, 37(2): 1-10.
	GUAN Z Z, ZHAO J N, QIU Q, et al. Growth law and optimal growth model of Catalpa bungei plantation: a case study of Catalpa bungei in Luoning County, Henan Province[J]. Forest Engineering, 2021, 37(2): 1-10.
[23]	XU L, WANG A A, WANG J, et al. Piriformospora indica confers drought tolerance on Zea mays L. through enhancement of antioxidant activity and expression of drought-related genes[J]. Crop J, 2017, 5(3): 251-258. DOI: 10.1016/j.cj.2016.10.002.
[24]	HOSSEINI F, MOSADDEGHI M R, DEXTER A R, et al. Maize water status and physiological traits as affected by root endophytic fungus Piriformospora indica under combined drought and mechanical stresses[J]. Planta, 2018, 247(5): 1229-1245. DOI: 10.1007/s00425-018-2861-6.
[25]	ZGALLAÏ H, STEPPE K, LEMEUR R. Effects of different levels of water stress on leaf water potential, stomatal resistance, protein and chlorophyll content and certain anti-oxidative enzymes in tomato plants[J]. J Integr Plant Biol, 2006, 48(6): 679-685. DOI: 10.1111/j.1744-7909.2006.00272.x.
[26]	任爱天, 鲁为华, 马春晖, 等. 接种AM真菌对紫花苜蓿抗旱性的影响[J]. 新疆农业科学, 2014, 51(9): 1677-1685.
	REN A T, LU W H, MA C H, et al. Effect of arbuscular mycorrhiza fungi on drought tolerance of Medicago sativa L.[J]. Xinjiang Agric Sci, 2014, 51(9): 1677-1685. DOI: 10.6048/j.issn.1001-4330.2014.09.016.

干旱胁迫 drought stress	株高/cm plant height		地径/mm ground diameter		干质量/g dry mass
干旱胁迫 drought stress	未定殖 no	定殖 yes	未定殖 no	定殖 yes	未定殖 no	定殖 yes
干旱drought	22.30±0.23 b	25.20±0.33 a	3.60±0.05 a	3.99±0.07 b	8.39±0.05 a	11.09±0.06 b
轻度干旱slight drought	24.71±0.17 a	25.91±0.28 a	4.01±0.09 a	4.17±0.16 a	11.20±0.12 a	11.70±0.17 a
正常供水normal irrigation	26.89±0.05 a	26.70±0.01 a	4.60±0.16 a	4.74±0.03 a	13.07±0.26 a	12.90±0.20 a

印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 26

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	马坛, 田野, 王书军, 李文昊, 段启英, 张庆源. 不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 172-180.
[2]	沈瑒, 狄晶晶, 陈颖, 冯凯, 陆锦玲, 胡宇辰. H₂S供体NaHS对渗透胁迫下美国剑麻干旱适应性及抗氧化特性的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 121-128.
[3]	杜晋城, 李欣欣, 王泽亮, 刘偲, 钟毅, 王丽华. 聚乙二醇胁迫下3个油橄榄品种生理指标响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 137-143.
[4]	刘佳磊, 白润娥, 张锴, 文才艺, 闫凤鸣. 我国桂花树上常见粉虱种类记述[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 237-244.
[5]	王良桂, 潘多, 丁卉芬, 施婷婷, 岳远征, 杨秀莲. 彩叶桂新品种‘南林彩云’[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 243-244.
[6]	张成, 王小燕, 王贤荣, 段一凡, 张敏, 施大伟, 朱跃, 宋炎峰, 柴子涵, 李岚. 雄全异株桂花不同花期光合和内源激素的变化[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 75-80.
[7]	康宏兴, 田清尹, 施婷婷, 岳远征, 杨秀莲, 王良桂. 彩叶桂花新品种‘南林彩锦’[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 247-248.
[8]	杨秀莲, 宰舟颖, 施婷婷, 岳远征, 王良桂. 彩叶桂新品种‘南林彩玉’[J]. 南京林业大学学报（自然科学版）, 2022, 46(3): 243-244.
[9]	臧德奎, 马燕, 王延玲, 鲍健, 鲍维, 鲍志贤. 桂花新品种‘金灿’[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 241-242.
[10]	李呈呈, 吴其超, 鲍健, 鲍维, 鲍志贤, 臧德奎, 马燕. 桂花新品种‘葱郁’[J]. 南京林业大学学报（自然科学版）, 2022, 46(1): 241-242.
[11]	段一凡, 李岚, 杨欣欣, 王贤荣, 张敏, 张成, 柴子涵. 桂花及其近缘种倍性和基因组大小分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 47-52.
[12]	吴其超, 马燕, 李呈呈, 许建军, 么燕君, 臧德奎. 桂花新品种‘冬荣’[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 245-246.
[13]	黎梦娟, 朱礼明, 霍俊男, 张景波, 施季森, 成铁龙. 唐古特白刺NtCBL1、NtCBL2基因克隆及表达分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 93-99.
[14]	洪震, 刘术新, 洪琮浩, 雷小华. 5种造林树种对干旱胁迫的抗性应答[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 111-119.
[15]	石欣隆, 杨月琴, 薛娴, 刘伟, 宋程威, 郭丽丽, 侯小改. 壳寡糖对干旱胁迫下‘凤丹’幼苗生长及生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 120-126.