印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

doi:10.12302/j.issn.1000-2006.202203014

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (2): 101-106.doi: 10.12302/j.issn.1000-2006.202203014

印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

母洪娜¹^,²(), 王炜², 樊蕾², 吴楚², 郭晓华², 孙陶泽¹^,²^,^*()

1.长江大学,湿地生态与农业利用教育部工程研究中心,湖北荆州 434025
2.长江大学园艺园林学院,湖北荆州 434025

收稿日期:2022-03-04 修回日期:2022-09-22 出版日期:2023-03-30 发布日期:2023-03-28
通讯作者: * 孙陶泽(suntaoze@yangtzeu.edu.cn),讲师。
基金资助:
湿地工程中心开放基金(KF201915);国家自然科学基金青年项目(51908063);湖北省教育厅哲学社会科学研究项目(21Y058);长江大学本科生“课程思政”项目(长江大学校发[2020]65号)

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

MU Hongna¹^,²(), WANG Wei², FAN Lei², WU Chu², GUO Xiaohua², SUN Taoze¹^,²^,^*()

1. Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou 434025, China
2. College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Received:2022-03-04 Revised:2022-09-22 Online:2023-03-30 Published:2023-03-28

摘要/Abstract

摘要：

【目的】研究了干旱胁迫下印度梨形孢(Piriformospora indica)对桂花(Osmanthus fragrans)的接种效应,为桂花节水低碳的栽培模式提供理论依据。【方法】以2年生桂花苗为材料,通过园土盆栽的方法,设置接种(40 mL含1 g新鲜印度梨形孢菌丝体的菌剂)+干旱(土壤含水量为田间最大持水量的20%~25%)、接种+轻度干旱(土壤含水量为田间最大持水量的45%~50%)、接种+正常供水(土壤含水量为田间最大持水量的65%~70%)、对照(40 mL灭活菌剂)+干旱、对照+轻度干旱、对照+正常供水共6个处理。【结果】印度梨形孢在桂花根系中定殖率高达81%。与对照组相比,接种印度梨形孢的桂花苗在轻度干旱和干旱胁迫下,株高、地径、干质量均高于对照,尤其是接种+干旱处理达到显著或极显著水平。与对照+干旱处理相比,接种后的桂花过氧化物酶(POD)与超氧化物歧化酶(SOD)、脯氨酸和可溶性糖含量分别增加了13.8%、37.9%、47.6%、40.7%。接种+干旱组的丙二醛含量比对照+干旱组增长量大幅降低。【结论】印度梨形孢与桂花共生能促进干旱(伴随夏季高温)胁迫下桂花生长,可能是其通过刺激SOD、POD活性增加和渗透调节物质的显著积累来抵御干旱胁迫;而轻度干旱和正常供水条件下,接种印度梨形孢对桂花生长无显著作用。

关键词: 印度梨形孢, 桂花, 干旱胁迫, 渗透调节, 抗旱性

Abstract:

【Objective】 The colonization effects of Piriformospora indica on Osmanthus fragrans under drought stress were analyzed. 【Method】 Two-year seedlings cultivated in pots with soil were used for this study. The experiment design was set up with the following parameters: inoculation (40 mL mycelium of P. indica) + drought (the soil water content is 20%-25% of the maximum field water capacity), inoculation + slight drought (45%-50% of the maximum field water capacity), inoculation + normal irrigation (65%-70% of the maximum field water capacity), control (40 mL inactivator) + drought, control + slight drought, and control + drought, six treatments in all. 【Result】 (1) Results showed that the colonization rate of P. indica was up to 81%. (2) Compared to the controls, the inoculated seedlings presented a better performance in plant height, stem diameter, and dry mass under both slight drought and drought stresses, especially the inoculation + drought groups, which showed significant or even extremely significant differences. (3) The protective enzyme activity of POD and SOD and the contents of proline and soluble sugar increased by 13.8%, 37.9%, 47.6% and 40.7%, respectively. (4) In addition, the MDA content decreased more quickly in the inoculation group than in the control group (control + drought). 【Conclusion】 This study found that the symbiosis of P. indica with O. fragrans played a positive role for O. fragrans seedlings in withstanding the negative effects of drought stress summer, most likely by stimulating the increase of SOD and POD enzyme activities and the significant accumulation of osmoregulatory substances. In contrast, inoculation with P. indica had no significant effect on the growth of O. fragrans under mild drought and normal water supply conditions.

Key words: Piriformospora indica, Osmanthus fragrans, drought stress, osmotic adjustment, drought resistance

中图分类号:

S685.13

母洪娜,王炜,樊蕾,等. 印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 101-106.

MU Hongna, WANG Wei, FAN Lei, WU Chu, GUO Xiaohua, SUN Taoze. Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(2): 101-106.DOI: 10.12302/j.issn.1000-2006.202203014.

图/表 5

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干旱胁迫 drought stress	株高/cm plant height		地径/mm ground diameter		干质量/g dry mass
干旱胁迫 drought stress	未定殖 no	定殖 yes	未定殖 no	定殖 yes	未定殖 no	定殖 yes
干旱drought	22.30±0.23 b	25.20±0.33 a	3.60±0.05 a	3.99±0.07 b	8.39±0.05 a	11.09±0.06 b
轻度干旱slight drought	24.71±0.17 a	25.91±0.28 a	4.01±0.09 a	4.17±0.16 a	11.20±0.12 a	11.70±0.17 a
正常供水normal irrigation	26.89±0.05 a	26.70±0.01 a	4.60±0.16 a	4.74±0.03 a	13.07±0.26 a	12.90±0.20 a

印度梨形孢对干旱胁迫下桂花生长及抗旱性的影响

Effects of Piriformospora indica on growth and drought resistance in Osmanthus fragrans under water deficit stress

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