两个南方红豆杉天然居群的交配系统分析

doi:10.12302/j.issn.1000-2006.202110046

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (5): 80-86.doi: 10.12302/j.issn.1000-2006.202110046

两个南方红豆杉天然居群的交配系统分析

罗芊芊¹^,²(), 李峰卿³, 肖德卿², 邓章文¹, 王建华¹, 周志春²^,^*()

1.抚州市林业科学研究所,江西抚州 344000
2.中国林业科学研究院亚热带林业研究所,浙江杭州 311400
3.中国林业科学研究院亚热带林业实验中心,江西分宜 336600

收稿日期:2021-10-23 修回日期:2022-05-12 出版日期:2023-09-30 发布日期:2023-10-10
通讯作者: *周志春(zczhou_caf@163.com),研究员。
作者简介:罗芊芊(luoqianqian617327@163.com),助理工程师。
基金资助:
百山祖国家公园科学研究项目(2021ZDLY02);中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2018ZB010);抚州市科技计划项目(2018Tc06)

Mating system analyses of two natural populations of Taxus wallichiana var. mairei

LUO Qianqian¹^,²(), LI Fengqing³, XIAO Deqing², DENG Zhangwen¹, WANG Jianhua¹, ZHOU Zhichun²^,^*()

1. Forestry Research Institute of Fuzhou,Fuzhou 344000,China
2. Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China
3. Experimental Center of Subtropical Forestry,Chinese Academy of Forestry,Fenyi 336600,China

Received:2021-10-23 Revised:2022-05-12 Online:2023-09-30 Published:2023-10-10

摘要/Abstract

摘要：

【目的】研究南方红豆杉(Taxus wallichiana var. mairei)天然居群的交配系统,揭示浙江龙泉和江西分宜天然居群的交配机制以及亲代间的遗传多样性差异,为南方红豆杉的科学保育和遗传资源有效利用提供理论参考。【方法】以浙江龙泉和江西分宜天然居群亲代及其子代为研究对象,运用12对SSR引物对71个亲代及8个采种母株的104个子代进行分析。【结果】参试的浙江龙泉和江西分宜居群亲代的等位基因数(N_a)皆为7,2个参试居群的有效等位基因数(N_e)分别为4.831和3.552,浙江龙泉居群较江西分宜居群高出1.279个有效等位基因。2个参试居群的观测杂合度(H_o)均小于期望杂合度(H_e),且固定指数(F)大于0。浙江龙泉居群的等位基因丰富度和私有等位基因丰富度(A_R=4.195,P_A=1.832)皆大于江西分宜居群(A_R=3.576,P_A=1.647)。2个参试居群的遗传多样性略有差异,但仍具有较高的遗传多样性。多位点交配系统分析(MLTR)结果表明,浙江龙泉和江西分宜居群中,南方红豆杉的异交率较高(t_m=1.200)。单位点和多位点父本相关性的差值[r_p(s)-r_p(m)]为0.095,亲代居群内存在近交现象,但近交水平较低( $C t m - t s$ =0.268)。有效花粉供体(N_ep=1.5)数目较少,表明南方红豆杉自然交配时亲本(父本)来源非常有限。【结论】南方红豆杉属于高度异交树种,2个参试居群的异交率差异不明显且遗传多样性皆较高,居群内存在近交现象,但近交水平较低。

关键词: 南方红豆杉, 天然居群, 交配系统, 亲代, 子代, 微卫星DNA

Abstract:

【Objective】 The objective of this study is to examine the mating system of Taxus wallichiana var. mairei in natural populations located in Longquan City from Zhejiang Province and Fenyi City from Jiangxi Province. Furthermore, the study aims to elucidate the influence of mating mechanisms and variances in parental genetic diversity, to provide a theoretical basis for the scientific conservation and efficient utilization of the genetic resources of T. wallichiana var. mairei. 【Method】 A total of 71 parental plants and 104 progenies stemming from eight mother plants were meticulously analyzed using 12 pairs of the SSR primers, all of which were sourced from natural populations located in Longquan of Zhejiang Province (ZJLQ) and Fenyi of Jiangxi Province (JXFY). With the aid of GenAlEx 6.5, a number of genetic diversity measures such as the observed alleles and effective alleles, as well as observed heterozygosity, expected heterozygosity, Shannon index and fixed index were meticulously calculated. Furthermore, the team also utilized HP-Rare 1.1 to calculate allele richness and private allele richness, while FSTAT 2.9.3 was utilized to test the genetic diversity parameters for any potential differences among parents within each population through 1 000 simulations, along with the calculation of the inbreeding coefficient of the parental population. Lastly, we used MLTR 3.4 to estimate thoroughly the genetic parameters of T. wallichiana var. mairei’s mating system, which included single-locus population outcrossing rate, multilocus population outcrossing rate, number of effective pollen donors, multilocus correlation of paternity, singlelocus correlation of paternity, difference between the paternal correlation of single-locus and multilocus.【Result】 The average allele of the parental generation from Longquan and Fenyi populations was 7. Meanwhile, the effective allele number (N_e), Shannon index (I), allele richness (A_R) and private allele richness (P_A) of the two parental populations were 4.192, 1.508, 3.886 and 1.740, respectively. In comparison, the ZJLQ population displayed higher N_e, I, A_R and P_A values than the JXFY population, standing at 4.831, 1.620, 4.195 and 1.832, respectively. Both populations displayed an inbreeding coefficient greater than zero, with the ZJLQ population’s (F_is= 0.057) coefficient being significantly lower than that of the JXFY population (F_is= 0.171). Additionally, the observed heterozygosity (H_o) of the both populations was lower than that of the expected heterozygosity (H_e), with the fixed index (F) being greater than zero, indicating a loss of heterozygotes. The fixed index (F) of the ZJLQ population was 0.001, which did not differ from zero. The Longquan population exhibited higher values for both allele richness and private allele richness (A_R= 4.195, P_A= 1.832) in comparison to the Fenyi population (A_R= 3.576, P_A= 1.647). Despite a slight difference in the genetic diversity of the two populations, they both displayed high levels of genetic diversity. Results from the multi-locus mating system analysis (MLTR) revealed that the outcrossing rate of T. wallichiana var. mairei was higher (t_m = 1.200) in the suitable populations of Longquan and Fenyi. Although the difference between paternal correlation of unit point and multilocus [r_p(s) - r_p(m)]equaled 0.095 and was greater than 0, indicating inbreeding in the parental population, the level of inbreeding was low (t_m-t_s = 0.268). The number of available pollen donors in both ZJLQ and JXFY populations was less than 2, and the paternal correlation and the number of effective pollen donors (N_ep=1/r_p(m)) were significantly different, suggesting that the parental sources were considerably limited during natural mating of T. wallichiana var. mairei. 【Conclusion】 T. wallichiana var. mairei is considered to be a highly outcrossing species, with both its single-locus population outcrossing rate and multilocus population outcrossing rate being high. However, there were no obvious differences in the outcrossing rate and genetic diversity between the two populations. Inbreeding was observed, but at low levels. The Longquan population is declining primarily due to the shortage of middle-aged and young plants and a high number of old trees. Despite the lower number of parental individuals in the ZJLQ population than the JXFY population, the former has a relatively high proportion of female individuals, which facilitates successful pollination rates and genetic exchange within the population. This advantageous characteristic results in the genetic diversity of the ZJLQ population not being inferior to that of the JXFY population. The Fenyi population has a larger number of young trees and seedlings, indicating that the habitat is suitable for the germination of T. wallichiana var. mairei seeds and the formation of seedlings. Therefore, with appropriate nurturing measures, this type of population has the potential to develop into an expanding population.

Key words: Taxus wallichiana var. mairei, natural population, mating system, parental generation, progeny, microsatellite(SSR)

中图分类号:

S722

罗芊芊,李峰卿,肖德卿,等. 两个南方红豆杉天然居群的交配系统分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 80-86.

LUO Qianqian, LI Fengqing, XIAO Deqing, DENG Zhangwen, WANG Jianhua, ZHOU Zhichun. Mating system analyses of two natural populations of Taxus wallichiana var. mairei[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(5): 80-86.DOI: 10.12302/j.issn.1000-2006.202110046.

图/表 6

图1

表1

表2

表3

图2

表4

2个南方红豆杉天然居群的交配系统参数"

居群 population	t_m	t_s	$C t m - t s$	r_p(m)	r_p(s)	r_p(s) - r_p(m)	N_ep
ZJLQ	1.200(0.000)	1.035(0.029)	0.165(0.029)	0.706(0.098)	0.501(0.111)	-0.205(0.078)	1.4
JXFY	1.200(0.000)	0.804(0.040)	0.396(0.040)	0.515(0.133)	0.999(0.121)	0.484(0.117)	1.9
总体水平total	1.200(0.000)	0.932(0.018)	0.268(0.018)	0.660(0.085)	0.755(0.098)	0.095(0.060)	1.5

表4

参考文献 40

[1]	ZHOU Y, CHEN G P, SU Y J, et al. Microsatellite loci from Taxus chinensis var. mairei (Taxaceae),an endangered and economically important tree species in China[J]. Front Biol China, 2009, 4(2):214-216.DOI: 10.1007/s11515-009-0013-x.
[2]	陈立新, 华波, 彭宝珠, 等. 南方红豆杉人工授粉与雌雄同株现象研究[J]. 现代农业科技, 2013(11):181,183.
	CHEN L X, HUA B, PENG B Z, et al. Study on artificial pollination and monoecious phenomenon of Taxus mairei[J]. Mod Agric Sci Technol, 2013(11): 181,183.DOI: 10.3969/j.issn.1007-5739.2013.11.120.
[3]	费永俊, 刘志雄, 王祥, 等. 南方红豆杉响应不同传粉式样的结实表现[J]. 西北植物学报, 2005, 25(3):478-483.
	FEI Y J, LIU Z X, WANG X, et al. Fruit settings of Taxus chinensis var. mairei in responding to different pollination types[J]. Acta Bot Boreali Occidentalia Sin, 2005, 25(3):478-483.DOI: 10.3321/j.issn:1000-4025.2005.03.010.
[4]	李先琨, 向悟生, 欧祖兰, 等. 濒危植物南方红豆杉种群克隆生长空间格局与动态[J]. 云南植物研究, 2003, 25(6):625-632.
	LI X K, XIANG W S, OU Z L, et al. Clonal growth spatial pattern and dynamics of the endangered plant Taxus mairei population[J]. Acta Bot Yunnanica, 2003, 25(6):625-632.DOI: 10.3969/j.issn.2095-0845.2003.06.001.
[5]	罗芊芊, 周志春, 邓宗付, 等. 南方红豆杉天然居群叶片的表型性状和氮磷化学计量特征的变异规律[J]. 植物资源与环境学报, 2021, 30(1):27-35.
	LUO Q Q, ZHOU Z C, DENG Z F, et al. Variation law of phenotypic traits and nitrogen and phosphorus stoichiometric characteristics of leaf of natural populations of Taxus wallichiana var. mairei[J]. J Plant Resour Environ, 2021, 30(1):27-35.DOI: 10.3969/j.issn.1674-7895.2021.01.04.
[6]	孙启武, 王磊, 张小平, 等. 皖南山区南方红豆杉种群动态研究[J]. 林业科学研究, 2009, 22(4):579-585.
	SUN Q W, WANG L, ZHANG X P, et al. Study on the population dynamics of Taxus chinensis var. mairei in the mountain area of southern Anhui Province[J]. For Res, 2009, 22(4):579-585.DOI: 10.3321/j.issn:1001-1498.2009.04.020.
[7]	高润梅, 石晓东, 樊兰英, 等. 山西省南方红豆杉自然分布与群落生态学特征[J]. 应用生态学报, 2016, 27(6):1820-1828.
	GAO R M, SHI X D, FAN L Y, et al. Natural distribution and community ecological characteristics of Taxus chinensis var.mairei in Shanxi Province,China[J]. Chin J Appl Ecol, 2016, 27(6):1820-1828.DOI: 10.13287/j.1001-9332.201606.029.
[8]	王敏求, 武星彤, 王利宝, 等. 南方红豆杉群体基因流比较分析[J]. 分子植物育种, 2019, 17(19):6269-6275.
	WANG M Q, WU X T, WANG L B, et al. Comparison of gene flow for Taxus chinensis var.mairei[J]. Mol Plant Breed, 2019, 17(19):6269-6275.DOI: 10.13271/j.mpb.017.006269.
[9]	WEN Y F, UCHIYAMA K, UENO S, et al. Assessment of the genetic diversity and population structure of Maire yew (Taxus chinensis var. mairei) for conservation purposes[J]. Can J For Res, 2018, 48(5):589-598.DOI: 10.1139/cjfr-2017-0429.
[10]	张志权, 廖文波, 钟翎, 等. 南方红豆杉种子萌发生物学研究[J]. 林业科学研究, 2000, 13(3):280-285.
	ZHANG Z Q, LIAO W B, ZHONG L, et al. Biological study on seed germination of Taxus mairei[J]. For Res, 2000, 13(3):280-285.DOI: 10.13275/j.cnki.lykxyj.2000.03.009.
[11]	张艳杰, 高捍东, 鲁顺保. 南方红豆杉种子中发芽抑制物的研究[J]. 南京林业大学学报(自然科学版), 2007, 31(4):51-56.
	ZHANG Y J, GAO H D, LU S B. Germination inhibitors in methanol extract from Taxus chinensis var. mairei seed[J]. J Nanjing For Univ (Nat Sci Ed), 2007, 31(4):51-56.DOI: 10.3969/j.issn.1000-2006.2007.04.011.
[12]	肖遥, 张蕊, 楚秀丽, 等. 24个产地南方红豆杉在两试验点的生长差异及其选择[J]. 林业科学研究, 2017, 30(2):342-348.
	XIAO Y, ZHANG R, CHU X L, et al. Growth variation and selection of Taxus wallichiana var. mairei from 24 locations in two trial plots[J]. For Res, 2017, 30(2):342-348.DOI: 10.13275/j.cnki.lykxyj.2017.02.022.
[13]	罗芊芊, 楚秀丽, 李峰卿, 等. 5年生南方红豆杉生长和分枝性状家系变异与选择[J]. 林业科学研究, 2020, 33(1):136-143.
	LUO Q Q, CHU X L, LI F Q, et al. Family variation and selection of growth and branching traits of 5-year-old Taxus wallichiana var. mairei[J]. For Res, 2020, 33(1):136-143.DOI: 10.13275/j.cnki.lykxyj.2020.01.018.
[14]	鲁定伟, 周要全, 李曙波, 等. 南方红豆杉生物学、生态学特性及培育技术[J]. 经济林研究, 2014, 32(4):159-164.
	LU D W, ZHOU Y Q, LI S B, et al. Biological characteristics,ecological characteristics and cultivation techniques in Taxus chinensis var. mairei[J]. Nonwood For Res, 2014, 32(4):159-164.DOI: 10.14067/j.cnki.1003-8981.2014.04.031.
[15]	姚甲宝, 袁小军, 周新华, 等. 南方红豆杉2年生容器苗育苗方案优选[J]. 东北林业大学学报, 2019, 47(11):11-16.
	YAO J B, YUAN X J, ZHOU X H, et al. Scheme optimization for big container seedlings of Taxus wallichiana var. mairei[J]. J Northeast For Univ, 2019, 47(11):11-16.DOI: 10.13759/j.cnki.dlxb.2019.11.003.
[16]	NEEL M C. Conservation implications of the reproductive ecology of Agalinis acuta (Scrophulariaceae)[J]. Am J Bot, 2002, 89(6):972-980.DOI: 10.3732/ajb.89.6.972.
[17]	FALCONER D S. Introduction to quantitative genetics[M].2nd ed. London: Longman, 1981.
[18]	FRANKEL O H, SOULÉ M E. Conservation and evolution[M]. Cambridge, London: Cambridge University Press, 1981.
[19]	赖焕林, 王章荣, 陈天华. 林木群体交配系统研究进展[J]. 世界林业研究, 1997, 10(5):10-15.
	LAI H L, WANG Z R, CHEN T H. Advances in research on mating systems of forest tree populations[J]. World For Res, 1997, 10(5):10-15.
[20]	徐刚标. 南方红豆杉濒危机制及保育技术[M]. 北京: 科学出版社, 2015:54-89.
	XU G B. Endangered mechanism and conversation for Taxus wallichiana var.mairei[M]. Beijing: Science Press, 2015:54-89.
[21]	罗芊芊. 南方红豆杉天然居群及家系遗传变异研究[D]. 长沙: 中南林业科技大学, 2020.DOI: 10.27662/d.cnki.gznlc.2020.000464.
	LUO Q Q. Genetic variation of Taxus wallichiana var.mairei natural populations and families[D]. Changsha: Central South University of Forestry & Technology, 2020.
[22]	易官美, 黎建辉, 王冬梅, 等. 南方红豆杉SSR分布特征分析及分子标记的开发[J]. 园艺学报, 2013, 40(3):571-578.
	YI G M, LI J H, WANG D M, et al. SSR distribution characteristic analysis and molecular marker development in Taxus wallichiana var. mairei[J]. Acta Hortic Sin, 2013, 40(3):571-578.DOI: 10.16420/j.issn.0513-353x.2013.03.031.
[23]	程蓓蓓. 中国红豆杉属分子谱系地理学与遗传多样性研究[D]. 北京: 中国林业科学研究院, 2016.
	CHENG B B. Molecular phylogeographic and genetic diversity of Taxus L.(taxaeae) in China[D]. Beijing: Chinese Academy of Forestry, 2016.
[24]	PEAKALL R, SMOUSE P E. GenAlEx 6.5:genetic analysis in Excel.population genetic software for teaching and research: an update[J]. Bioinformatics, 2012, 28(19):2537-2539.DOI: 10.1093/bioinformatics/bts460.
[25]	KALINOWSKI S T. Hp-rare 1.0: a computer program for performing rarefaction on measures of allelic richness[J]. Mol Ecol Notes, 2005, 5(1):187-189.DOI: 10.1111/j.1471-8286.2004.00845.x.
[26]	GOUDET J. FSTAT (version 1.2): a computer program to calculate F-statistics[J]. J Hered, 1995, 86(6):485-486.DOI: 10.1093/oxfordjournals.jhered.a111627.
[27]	王霞, 王静, 蒋敬虎, 等. 观光木片断化居群的遗传多样性和交配系统[J]. 生物多样性, 2012, 20(6):676-684. doi: 10.3724/SP.J.1003.2012.09083
	WANG X, WANG J, JIANG J H, et al. Genetic diversity and the mating system in a fragmented population of Tsoongiodendron odorum[J]. Biodivers Sci, 2012, 20(6):676-684.DOI: 10.3724/SP.J.1003.2012.09083.
[28]	RITLAND K. Extensions of models for the estimation of mating systems using n independent loci[J]. Heredity, 2002, 88(4):221-228.DOI: 10.1038/sj.hdy.6800029. pmid: 11920127
[29]	RITLAND K. Correlated matings in the partial selfer Mimulus guttatus[J]. Evolution, 1989, 43(4):848-859.DOI: 10.1111/j.1558-5646.1989.tb05182.x.
[30]	ALLENDORF F W, LUIKART G. Conservation and the genetics of populations[M]. Malden,MA: Blackwell Pub, 2007.
[31]	徐刚标. 植物群体遗传学[M]. 北京: 科学出版社, 2009:39-68.
	XU G B. Plant population genetics[M]. Beijing: Science Press, 2009:39-68.
[32]	杨汉波, 张蕊, 周志春. 木荷种子园的遗传多样性和交配系统[J]. 林业科学, 2016, 52(12):66-73.
	YANG H B, ZHANG R, ZHOU Z C. Genetic diversity and mating system in a seed orchard of Schima superba[J]. Sci Silvae Sin, 2016, 52(12):66-73.DOI: 10.11707/j.1001-7488.20161208.
[33]	谭小梅, 周志春, 金国庆, 等. 马尾松二代无性系种子园遗传多样性和交配系统分析[J]. 林业科学, 2012, 48(2):69-74.
	TAN X M, ZHOU Z C, JIN G Q, et al. Genetic diversity and mating system analysis of Pinus massoniana in a second-generation clonal seed orchard[J]. Sci Silvae Sin, 2012, 48(2):69-74.DOI: 10.11707/j.1001-7488.20120210.
[34]	DERING M, MISIORNY A, CHALUPKA W. Inter-year variation in selfing,background pollination,and paternal contribution in a Norway spruce clonal seed orchard[J]. Can J For Res, 2014, 44(7):760-767.DOI: 10.1139/cjfr-2014-0061.
[35]	WEI Z G, QU Z, HOU C, et al. Genetic diversity and paternal analysis of open- pollinated progenies of Larix olgensis seed orchard[J]. J Nat Sci, 2015, 1(1):19-24.
[36]	TAMAKI I, SETSUKO S, TOMARU N. Estimation of outcrossing rates at hierarchical levels of fruits,individuals,populations and species in Magnolia stellata[J]. Heredity, 2009, 102(4):381-388.DOI: 10.1038/hdy.2008.128.
[37]	何亚平, 费世民, 徐嘉, 等. 四川麻疯树花序结构和雌雄花动态研究[J]. 四川林业科技, 2008, 29(2):1-8.
	HE Y P, FEI S M, XU J, et al. The inflorescence structure and dynamics of male and female flowers of Jatropha curcas in Sichuan Province[J]. J Sichuan For Sci Technol, 2008, 29(2):1-8.DOI: 10.16779/j.cnki.1003-5508.2008.02.001.
[38]	CHARLESWORTH D, CHARLESWORTH B. Inbreeding depression and its evolutionary consequences[J]. Annu Rev Ecol Syst, 1987, 18:237-268.DOI: 10.1146/annurev.es.18.110187.001321.
[39]	SCHEMSKE D W, HUSBAND B C, RUCKELSHAUS M H, et al. Evaluating approaches to the conservation of rare and endangered plants[J]. Ecology, 1994, 75(3):584-606.DOI: 10.2307/1941718.
[40]	周志春, 余能健, 金国庆. 南方红豆杉和三尖杉药用种质选择及高效栽培[M]. 北京: 中国林业出版社, 2009:41-57.
	ZHOU Z C, YU N J, JIN G Q. Medicinal variety selection and efficient cultivation of Taxus wallichiana var.mairei and Cephalotaxus fortunei[M]. Beijing: China Forestry Publishing House, 2009:41-57.

居群代号 population code	采样地 seed collection site	东经 longtitude (E)	北纬 latitude (N)	海拔/m altitude	亲代数目 number of adults	亲代性别比例(♀/♂) female/male of parental plants	采种母株数 number of mother plants collected	采集叶样的子代数 number of progeny of leaf samples collected
ZJLQ	浙江龙泉 Longquan,Zhejiang	118°47'48″	28°0'36″	585~772	22	13/9	5	78
JXFY	江西分宜 Fenyi, Jiangxi	114°32'39″	27°37'17″	241~624	49	6/43	3	26

引物 primer	序列sequence(5'-3')	片段大小/bp size range	退火温度/℃ annealing temperature	参考文献 reference
S7	F:ACCCCATAGTTCAGGGTCGA	149~292	57	[23]
	R:AGAAGGAGGCTCGGCTCTAA
S24	F:TGCTTAGAGAAGAAAGTTTTCCA	145~312	54	[22]
	R:TGGCATACAAGAGGCTGACA
S34	F:ATAACCATCACACGTGACTTGC	193~300	57	[22]
	R:TGAACCCTAGGTGACGACCAT
S36	F:TGGTTTACATATTGAAGCGAGCAA	109~176	55	[22]
	R:GCAGCGAAACATATGTAGCACAA
S41	F:GGACAAGGGTTGGATCACTTCTGT	203~294	61	[22]
	R:CCAACGGCCACCCGAAGAGT
S44	F:TGACAACACAACCACACAATGTCCA	133~375	57	[22]
	R:TCTTGTTTTCTGGGCCTAAAGTGT
S45	F:GCGCTAGTCCTCAGTGGTGCC	134~272	63	[22]
	R:GCGCCGGGGCCAACTAAACT
S64	F:GCATTCTGCTCCTGAGTGTGGCA	223~397	61	[22]
	R:AGTAGTCTATCCTCGTCTCCTCCCA
S77	F:CCAATGTGGGCTACATCCACC	178~357	58	[22]
	R:ATTGTAATAGCATGGATAAGTGCCC
S79	F:AGTTTGCATGCTCTTCAACCTAGT	176v249	57	[22]
	R:AGGCAGAATCGGTGAGTGGTT
S82	F:TGTCGCATCGAGGACGATGCTTTC	201~434	62	[22]
	R:CATGGCGGCGGCAGTTCTTG
S93	F:CAGGGCTCAAATTCGCGGGC	147~195	62	[22]
	R:CCGCCTGGCGTTTGACAGGA

两个南方红豆杉天然居群的交配系统分析

Mating system analyses of two natural populations of Taxus wallichiana var. mairei

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

居群 population	样本数目 number of samples	N_a	N_e	A_R	P_A	H_o	H_e	I	F	F_is
ZJLQ	22	7	4.831	4.195	1.832	0.726	0.749	1.620	0.001	0.057
JXFY	49	7	3.552	3.576	1.647	0.568	0.677	1.395	0.156	0.171
均值mean	36	7	4.192	3.886	1.740	0.647	0.713	1.508	0.079	0.114

[1]	欧建德. 杈干现象对南方红豆杉树冠形态、生长和形质的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 87-94.
[2]	叶代全. 杉木第4代育种候选群体的12年生全同胞子代测定表现与选择[J]. 南京林业大学学报（自然科学版）, 2022, 46(6): 240-250.
[3]	张庆源, 田野, 王淼, 翟政, 周诗朝. 美洲黑杨与青杨杂交F₁代苗期表型性状的分化及其类型划分[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 40-48.
[4]	李丹丹, 翁启杰, 甘四明, 周长品, 黄世能, 李梅. 基于EST-SSR标记鉴定猴耳环自由授粉的全同胞子代[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 95-101.
[5]	袁金玲, 马婧瑕, 钟远标, 岳晋军. 基于SSR标记的丛生竹杂种鉴定、遗传分析和指纹图谱构建[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 10-18.
[6]	陈黎, 刘成功, 钱莹莹, 唐晓蝶, 王生树, 李志东, 李燕, 崔珺. 南方红豆杉人工林针叶C、N、P化学计量特征[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 53-61.
[7]	彭洋, 陆跃堂, 赵杨, 肖枫. 棕榈半同胞子代家系苗期测定及优良家系选择[J]. 南京林业大学学报（自然科学版）, 2020, 44(5): 78-84.
[8]	林源, 陈培, 周明明, 尚旭岚, 方升佐. 天然居群青钱柳叶主要生物活性物质及抗氧化活性研究[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 10-16.
[9]	李峰卿, 王秀花, 楚秀丽, 张东北, 吴小林, 周生财, 叶明. 缓释肥N/P比及加载量对5种珍贵树种1年生苗生长和养分库构建的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(1): 72-80.
[10]	欧建德,吴志庄. 幼龄南方红豆杉人工林树冠形态特征与生长形质通径分析[J]. 南京林业大学学报（自然科学版）, 2019, 43(04): 185-191.
[11]	张晶,浦静,赵聪,姜文龙,范俊俊, 穆茜,杨祎凡,王志东,张往祥. 3个观赏海棠半同胞家系子代叶色动态分析与初选[J]. 南京林业大学学报（自然科学版）, 2018, 42(03): 37-44.
[12]	谭健晖,冯源恒,黄永利,杨章旗. 26年生马尾松初级种子园半同胞子代变异及家系选择[J]. 南京林业大学学报（自然科学版）, 2017, 41(03): 189-192.
[13]	肖遥,张蕊,刘建慧,梁朔,周志春. 不同产地南方红豆杉育种亲本叶绿素含量及荧光参数差异分析[J]. 南京林业大学学报（自然科学版）, 2017, 41(03): 57-64.
[14]	欧建德,吴志庄. 南方红豆杉人工林树干干形异常的诊断及处置[J]. 南京林业大学学报（自然科学版）, 2017, 41(03): 95-99.
[15]	欧建德,吴志庄. 南方红豆杉修枝经营措施优化及评价[J]. 南京林业大学学报（自然科学版）, 2017, 41(01): 117-122.