基于广泛靶向代谢组学技术的勃氏甜龙竹竹叶化学成分分析

doi:10.12302/j.issn.1000-2006.202204026

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 241-246.doi: 10.12302/j.issn.1000-2006.202204026

基于广泛靶向代谢组学技术的勃氏甜龙竹竹叶化学成分分析

王飞¹(), 谢瞰¹, 杨亚晋¹, 刘莉莉¹, 陈粉粉¹, 李恩良², 郭爱伟¹^,³^,^*()

1.西南林业大学生命科学学院,云南昆明 650224
2.西岐桫椤省级自然保护区管护局,云南墨江 654800
3.云南省高校林木生物技术重点实验室,云南昆明 650224

收稿日期:2022-04-11 修回日期:2022-06-27 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *郭爱伟(g.aiwei.swfu@hotmail.com),教授。
作者简介:王飞(674283334@qq.com)。
基金资助:
云南省优势特色重点学科生物学一级学科建设项目(50097505);云南省教育厅研究生基金项目(111722056);国家自然科学基金项目(31860650)

Analysis of chemical components in Dendrocalamus brandisii leaves based on extensive widely targeted metabolomics

WANG Fei¹(), XIE Kan¹, YANG Yajin¹, LIU Lili¹, CHEN Fenfen¹, LI Enliang², GUO Aiwei¹^,³^,^*()

1. College of Life Science,Southwest Forestry University,Kunming 650224,China
2. Xiqi Tree-fern Provincial Nature Reserve Management and Conservation Bureau,Mojiang 654800,China
3. Yunnan University Key Laboratory of Forest Biotechnology,Kunming 650224,China

Received:2022-04-11 Revised:2022-06-27 Online:2024-03-30 Published:2024-04-08

1. 林业2402王飞附表.pdf(379KB)

摘要/Abstract

摘要：

【目的】探明勃氏甜龙竹(Dendrocalamus brandisii)竹叶化学成分,准确认识勃氏甜龙竹竹叶的应用价值,为其开发和利用提供科学依据。【方法】采用Waters ACQUITY UPLC HSS T3 C18(2.1 mm×100 mm,1.8 μm)液相色谱柱,在0.35 mL/min的流速下,以A相为0.04%(体积分数,下同)的乙酸水溶液、B相为0.04%的乙酸乙腈溶液进行洗脱,并选取电喷雾离子源在三重四级杆中,根据优化的去簇电压和碰撞能下扫描检测;利用武汉迈特维尔生物科技有限公司自建数据库,根据二级谱信息进行物质定性,利用三重四级杆质谱MRM对代谢物进行定量,通过对化合物特征离子色谱峰面积的积分和校正,确定各物质的相对含量。【结果】在勃氏甜龙竹竹叶中共检测到10大类407种代谢物,其中黄酮类化合物79种,占检测化合物总数的19.41%;脂质类61种,占检测化合物总数的14.99%;酚酸类55种,占检测化合物总数的13.51%;氨基酸及其衍生物55种,占检测化合物总数的13.51%;有机酸类35种,占检测化合物总数的8.60%;核苷酸及其衍生物31种,占检测化合物总数的7.62%;生物碱类28种,占检测化合物总数的6.88%;木脂素和香豆素类17种,占检测化合物总数的4.18%;萜类1种,占检测化合物总数的0.24%;其他类化合物45种,占检测化合物总数的11.06%。【结论】基于广泛靶向代谢组学技术对勃氏甜龙竹竹叶化合物类型和相对含量分析结果表明,除基本营养组成外,勃氏甜龙竹竹叶中含有多种药用价值的化合物,其中黄酮类化合物是勃氏甜龙竹竹叶中的主要活性成分之一,在人类健康和动物保健领域具有广阔的开发和应用前景。本研究为进一步开发和利用勃氏甜龙竹竹叶中的药用活性物质提供科学依据。

关键词: 勃氏甜龙竹, 竹叶, 化学成分, 代谢物, 广泛靶向代谢组

Abstract:

【Objective】 This research aims to reveal the chemical components of the leaves of Dendrocalamus brandisii and accurately identify the application value of these leaves, hoping to provide a scientific basis for the development and utilization of this species. 【Method】 The research employed a Waters ACQUITY UPLC HSS T3 C18 (2.1 mm × 100 mm, 1.8 μm) liquid chromatography column. Elution was conducted at a flow rate of 0.35 mL/min using a 0.04% (volume fraction, same as below)aqueous acetic acid solution as phase A and a 0.04% acetic acid acetonitrile solution as phase B. The electrospray ion source was selected in a triple quadrupole, where detection was scanned under optimized declustering voltage and collision energy. The self-built database of Wuhan Metware Biotechnology Co., Ltd. was used to qualitatively identify substances based on secondary spectrum information. Metabolites were quantified using a triple quadrupole mass spectrometer (MRM), with the relative content of each substance determined by integrating and correcting compound characteristic ion chromatographic peak areas. 【Result】 The 407 metabolites were detected in ten categories, including 79 flavonoids, accounting for 19.41% of the total compounds. There were 61 types of lipids and 55 phenolic acids, accounting for 14.99% and 13.51% of the total detected compounds, respectively. There were 55 types of amino acids and their derivatives and 35 organic acids, accounting for 13.51% and 8.60% of the total detected compounds, respectively. Thirty-one nucleotides and their derivatives accounted for 7.62% of the total detected compounds, and 28 alkaloids accounted for 6.88% of the total detected compounds. There were 17 lignans and coumarins, accounting for 4.18% of the total detected compounds. One terpene accounted for 0.24% of the total detected compounds. There were 45 other compounds, accounting for 11.06% of the total detected compounds. 【Conclusion】 Besides basic nutritional components, these leaves contain many medicinal compounds. Flavonoid compounds, in particular, serve as one of the primary active ingredients in these leaves. These compounds have significant development and application prospects in human health and animal care. This study provides a scientific basis for further development and utilization of medicinally active substances present in the leaves of Dendrocalamus brandisii.

Key words: Dendrocalamus burmannii, bamboo leaf, chemical composition, metabolites, widely targeted metabolomics

中图分类号:

S795

王飞,谢瞰,杨亚晋,等. 基于广泛靶向代谢组学技术的勃氏甜龙竹竹叶化学成分分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 241-246.

WANG Fei, XIE Kan, YANG Yajin, LIU Lili, CHEN Fenfen, LI Enliang, GUO Aiwei. Analysis of chemical components in Dendrocalamus brandisii leaves based on extensive widely targeted metabolomics[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 241-246.DOI: 10.12302/j.issn.1000-2006.202204026.

图/表 1

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基于广泛靶向代谢组学技术的勃氏甜龙竹竹叶化学成分分析

Analysis of chemical components in Dendrocalamus brandisii leaves based on extensive widely targeted metabolomics

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