黄连木变色期叶片色素变化规律及呈色机理

doi:10.12302/j.issn.1000-2006.202210034

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (2): 97-104.doi: 10.12302/j.issn.1000-2006.202210034

黄连木变色期叶片色素变化规律及呈色机理

许志钊(), 杨秀云^*(), 王祎琛, 杜淑辉

山西农业大学林学院,山西太谷 030801

收稿日期:2022-10-26 修回日期:2023-01-30 出版日期:2024-03-30 发布日期:2024-04-08
通讯作者: *杨秀云(xyyang2002@yeah.net),教授。
作者简介:许志钊(846939322@qq.com)。
基金资助:
山西省基础研究计划项目(202103021224144);山西农业大学生物育种工程项目(YZGC138)

Changes in pigment and coloration mechanism of leaves during the discoloration period of Pistacia chinensis

XU Zhizhao(), YANG Xiuyun^*(), WANG Yichen, DU Shuhui

College of Forestry, Shanxi Agricultural University, Taigu 030801, China

Received:2022-10-26 Revised:2023-01-30 Online:2024-03-30 Published:2024-04-08

摘要/Abstract

摘要：

【目的】研究黄连木(Pistacia chinensis)秋季叶片变色期相关生理指标含量变化,揭示其叶色变化与生理特征间的内在规律。【方法】以变色期的黄连木为研究对象,对其叶色参数、色素、渗透物质、全氮、全磷含量和关键酶活性变化进行定量测定及分析。【结果】变色期黄连木叶片中叶绿素和类胡萝卜素含量不断下降,花青素含量增多,叶片变为红色;黄连木变色期叶片中花青素与色相参数a^*值、类胡萝卜素与叶绿素质量比、花青素与叶绿素质量比、可溶性糖、游离氨基酸含量、苯丙氨酸解氨酶和花青素合成酶活性极显著正相关(P<0.01),与二氢黄酮醇-4-还原酶活性显著正相关(P<0.05),与全氮含量显著负相关(P<0.05),与全磷含量极显著负相关(P<0.01)。【结论】黄连木秋季变色期叶片中花青素与叶绿素质量比及花青素含量在变色中期剧增是导致叶片变红的物质基础,可溶性糖含量、苯丙氨酸解氨酶活性是影响呈色的关键因子,同时,变色期矿质元素氮和磷含量显著降低,共同促进花青素合成进而对叶色产生影响。

关键词: 黄连木, 变色期, 花青素, 呈色机理

Abstract:

【Objective】 The changes of physiological substances related to the discoloration period of the leaves of Pistacia chinensis in autumn were studied to reveal the regularity between leaf color and content.【Method】 The experiment was conducted to quantify and analyze the changes in leaf color parameters, pigments, osmotic substances, total nitrogen and phosphorus contents and the key enzyme activities of P. chinensis.【Result】 During the discoloration period, the contents of chlorophyll and carotenoid in leaves continuously decreased, the content of anthocyanins increased, and the leaves turned red. Anthocyanins in the leaves of P. chinensis showed extremely significantly positive correlations with a^* values, the ratio of carotenoid to chlorophyll and anthocyanin to chlorophyll, soluble sugar, free amino acid, phenylalanine ammonialyase(PAL) and anthocyanidin synthase(ANS) (P<0.01). Anthocyanins were positively correlated with dihydroflavonol-4-reductase(DFR) (P<0.05). Anthocyanins were negatively correlated with nitrogen(N) (P<0.05). Anthocyanins were extremely significantly negatively correlated with phosphorus(P) (P<0.01). 【Conclusion】 The anthocyanin/chlorophyll ratio and anthocyanin content in the leaves during the autumn discoloration period of P. chinensis are the material basis for leaf reddening in the middle stage of discoloration. Soluble sugar content and PAL enzyme activity are the key factors affecting color. Mineral elements (nitrogen and phosphorus) content in the discoloration period was significantly reduced, promoting anthocyanin synthesis and affecting leaf color.

Key words: Pistacia chinensis, discoloration period, anthocyanins, coloration mechanism

中图分类号:

S687.9

许志钊,杨秀云,王祎琛,等. 黄连木变色期叶片色素变化规律及呈色机理[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 97-104.

XU Zhizhao, YANG Xiuyun, WANG Yichen, DU Shuhui. Changes in pigment and coloration mechanism of leaves during the discoloration period of Pistacia chinensis[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(2): 97-104.DOI: 10.12302/j.issn.1000-2006.202210034.

图/表 7

图1

图2

图3

图4

图5

表1

图6

参考文献 46

[1]	王祎琛, 毛俊俨, 陈星京, 等. 应用最大熵模型预测黄连木在中国的潜在分布[J]. 东北林业大学学报, 2021, 49(7):61-65.
	WANG Y C, MAO J Y, CHEN X J, et al. Predicting potential distribution of Pistacia chinensis in China using MaxEnt model[J]. J Northeast For Univ, 2021, 49(7):61-65.DOI: 10.13759/j.cnki.dlxb.2021.07.011.
[2]	田会敏, 代三花. 黄连木的园林特性及开发利用[J]. 现代园艺, 2013(6):114,116.
	TIAN H M, DAI S H. Landscape characteristics and development and utilization of Pistacia chinensis[J]. Xiandai Hortic, 2013(6): 114,116.DOI:10.14051/j.cnki.xdyy.2013.06.142
[3]	CHAPPELL J, HAHLBROCK K. Transcription of plant defence genes in response to UV light or fungal elicitor[J]. Nature, 1984, 311(5981):76-78.DOI: 10.1038/311076a0.
[4]	SHI M Z, XIE D Y. Biosynthesis and metabolic engineering of anthocyanins in Arabidopsis thaliana[J]. Recent Pat Biotechnol, 2014, 8(1):47-60.DOI: 10.2174/1872208307666131218123538.
[5]	王晰, 丁文杰, 李娅, 等. 长筒石蒜花青素合成酶基因LlANS的克隆与表达分析[J]. 河南农业大学学报, 2018, 52(4): 611-617.
	WANG X, DING W J, LI Y, et al. Cloning and expression analysis of anthocyanidin synthase (LlANS) in Lycoris longituba[J]. J Henan Agricl Univ, 2018, 52(4):611-617.DOI: 10.16445/j.cnki.1000-2340.2018.04.020.
[6]	YOSHIHARA N, IMAYAMA T, FUKUCHI-MIZUTANI M, et al. cDNA cloning and characterization of UDP-glucose:anthocyanidin 3-O-glucosyltransferase in Iris hollandica[J]. Plant Sci, 2005, 169(3):496-501.DOI: 10.1016/j.plantsci.2005.04.007.
[7]	张少露. 红叶石楠叶色变化过程中叶结构和生理特征研究[D]. 雅安: 四川农业大学, 2017.
	ZHANG S L. Preliminary study on leaf structure and physiological and biochemical characteristics of Photinia frasery during leaf color change[D]. Ya'an: Sichuan Agricultural University, 2017.
[8]	胡静静, 沈向, 李雪飞, 等. 黄连木秋季叶色变化与可溶性糖和矿质元素的关系[J]. 林业科学, 2010, 46(2):80-86.
	HU J J, SHEN X, LI X F, et al. Relationships of leaf color changes and soluble sugars and mineral elements in leaves of Pistacia chinensis in autumn[J]. Sci Silvae Sin, 2010, 46(2):80-86.DOI: 10.11707/j.1001-7488.20100214.
[9]	郭欢欢, 刘勇, 姚飞, 等. 不同种源黄连木秋季色素含量与叶色参数的关系[J]. 西北植物学报, 2017, 37(10):2003-2009.
	GUO H H, LIU Y, YAO F, et al. Relationship between pigment contents and leaf color parameters of Pistacia chinensis Bunge of different provenances in autumn[J]. Acta Bot Boreali Occidentalia Sin, 2017, 37(10):2003-2009.DOI: 10.7606/j.issn.1000-4025.2017.10.2003.
[10]	SONG X H, DUAN X J, CHANG X C, et al. Molecular and metabolic insights into anthocyanin biosynthesis during leaf coloration in autumn[J]. Environ Exp Bot, 2021, 190:104584.DOI: 10.1016/j.envexpbot.2021.104584.
[11]	李力, 张盛楠, 刘亚敏, 等. 基于Lab模型的北美红枫呈色生理因素探究[J]. 西北农林科技大学学报(自然科学版), 2017, 45(9):87-94.
	LI L, ZHANG S N, LIU Y M, et al. Lab model based analysis on physiological factors affecting color of Acer rubrum L.[J]. J Northwest A F Univ (Nat Sci Ed), 2017, 45(9):87-94.DOI: 10.13207/j.cnki.jnwafu.2017.09.012.
[12]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000:134-137.
	LI H S. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000:134-137.
[13]	李小方, 张志良. 植物生理学实验指导[M]. 5版. 北京: 高等教育出版社, 2016:124-125.
	LI X F, ZHANG Z L. Experimental instruction of plant physiology[M]. 5th ed. Beijing: Higher Education Press, 2016:124-125.
[14]	张志良, 瞿伟菁. 植物生理学实验指导[M]. 3版. 北京: 高等教育出版社, 2003.
	ZHANG Z L, QU W J. The experimental guide for plant physiology[M]. 3rd ed. Beijing: Higher Education Press, 2003.
[15]	路文静, 李奕松. 植物生理学实验教程[M]. 北京: 中国林业出版社, 2012:143-144.
	LU W J, LI Y S. Experimental course of plant physiology[M]. Beijing: China Forestry Publishing House, 2012:143-144.
[16]	王晶英, 敖红, 张杰, 等. 植物生理生化实验技术与原理[M]. 哈尔滨: 东北林业大学出版社, 2003:165-166.
	WANG J Y, AO H, ZHANG J, et al. Techniques and principle of plant physiology and biochemistry experiment[M]. Harbin: Northeast Forestry University Press, 2003:165-166.
[17]	LOWTHER J R. Use of a single sulphuric acid-hydrogen peroxide digest for the analysis of Pinus radiata needles[J]. Commun Soil Sci Plant Anal, 1980, 11(2):175-188.DOI: 10.1080/00103628009367026.
[18]	蔡永萍. 植物生理学实验指导[M]. 北京: 中国农业大学出版社, 2014.
	CAI Y P. Experimental instruction of plant physiology[M]. Beijing: China Agricultural University Press, 2014.
[19]	陈慧. 秀丽香港四照花叶变色期生理特性研究[D]. 杭州: 浙江农林大学, 2017.
	CHEN H. The research on the physiological characteristics of Dendrobenthamia hongkongensis ssp.elegans[D]. Hangzhou: Zhejiang A&F University, 2017.
[20]	张敏, 黄利斌, 周鹏, 等. 榉树秋季转色期叶色变化的生理生化[J]. 林业科学, 2015, 51(8):44-51.
	ZHANG M, HUANG L B, ZHOU P, et al. Physiological and biochemical changes in Zelkova serrata leaves during leaf color transformation in autumn[J]. Sci Silvae Sin, 2015, 51(8):44-51.DOI: 10.11707/j.1001-7488.20150806.
[21]	郭亚林. 红叶杨叶片呈色机理初探[D]. 雅安: 四川农业大学, 2013.
	GUO Y L. Preliminary study on the chromogenic mechanism of leaf in red-leaf poplars[D]. Ya'an: Sichuan Agricultural University, 2013.
[22]	江洁蓓, 梁玲, 张腾驹, 等. 粉红珙桐叶片呈色相关生理特性的季节变化[J]. 西北植物学报, 2019, 39(11):2019-2027.
	JIANG J B, LIANG L, ZHANG T J, et al. Seasonal dynamic of physiological characteristics of pink Davidia involucrata[J]. Acta Bot Boreali Occidentalia Sin, 2019, 39(11):2019-2027.DOI: 10.7606/j.issn.1000-4025.2019.11.2019.
[23]	赵东辉, 高玉福, 荣立苹, 等. 紫花槭秋季叶片呈色生理变化研究[J]. 经济林研究, 2019, 37(2):114-119.
	ZHAO D H, GAO Y F, RONG L P, et al. Study on physiological change of Acer pseudo-sieboldianum leaf color in autumn[J]. Non Wood For Res, 2019, 37(2):114-119.DOI: 10.14067/j.cnki.1003-8981.2019.02.016.
[24]	齐睿, 李小红, 石博雨, 等. 红叶石楠转色期叶片色彩参数与色素含量的相关性分析[J]. 河南农业科学, 2019, 48(4):93-101.
	QI R, LI X H, SHI B Y, et al. Analysis on the relationship of leaf color parameters and pigments contents in Photinia × fraseri dress with veraison stage[J]. J Henan Agric Sci, 2019, 48(4):93-101.DOI: 10.15933/j.cnki.1004-3268.2019.04.015.
[25]	史宝胜. 紫叶李叶色生理变化及影响因素研究[D]. 哈尔滨: 东北林业大学, 2006.
	SHI B S. The research on the physiological characters and the influence factors on leave color of purple leaf cherry plum[D]. Harbin: Northeast Forestry University, 2006.
[26]	PALLARDY S G. Physiology of woody plants[M]. Pittsburgh, USA: Academic Press, 2010.
[27]	安然, 王树凤, 陈益泰, 等. 栎树秋季叶色多样性及相关生理特性的变化[J]. 林业科学研究, 2022, 35(2):78-88.
	AN R, WANG S F, CHEN Y T, et al. Leaf color diversity and the change of physiological characteristics of Quercus spp.during the discoloration period in autumn[J]. For Res, 2022, 35(2):78-88.DOI: 10.13275/j.cnki.lykxyj.2022.02.009.
[28]	聂庆娟, 史宝胜, 孟朝, 等. 不同叶色红栌叶片中色素含量、酶活性及内含物差异的研究[J]. 植物研究, 2008, 28(5):599-602.
	NIE Q J, SHI B S, MENG Z, et al. The enzyme activities,pigment and inclusion contents in different leaves color of Cotinus coggygria ‘Royal Purple’ in autumn[J]. Bull Bot Res, 2008, 28(5):599-602.
[29]	唐生森, 陈虎, 覃永康, 等. 枫香秋季变色期叶色变化及其生理基础[J]. 广西植物, 2021, 41(12):2061-2068.
	TANG S S, CHEN H, QIN Y K, et al. Physiological basis of Liquidambar formosana leaves during leaf color transformation in autumn[J]. Guihaia, 2021, 41(12):2061-2068.
[30]	陈芳. 金叶白蜡叶片呈色机理的研究[D]. 保定: 河北农业大学, 2013.
	CHEN F. Study on the leaf chromogenic mechanism of Fraxinus linn[D]. Baoding: Hebei Agricultural University, 2013.
[31]	李向茂. 小丑火棘秋冬转色期叶色变化的生理特性[J]. 上海交通大学学报(农业科学版), 2013, 31(3):82-87.
	LI X M. Physiological characters of Pyracantha fortuneana ‘Harlequin’ leaves during color-changing period in autumn and winter[J]. J Shanghai Jiao Tong Univ (Agric Sci), 2013, 31(3):82-87.DOI: 10.3969/J.ISSN.1671-9964.2013.03.014.
[32]	朱亮, 顾国军, 徐振锋, 等. 北亚热带常绿阔叶林乔灌草物种的碳氮磷生态化学计量比[J]. 应用与环境生物学报, 2019, 25(6):1277-1285.
	ZHU L, GU G J, XU Z F, et al. Ecological stoichiometric ratio of carbon,nitrogen,and phosphorus in tree,shrub,and herb species in a subtropical evergreen broad-leaved forest[J]. Chin J Appl Environ Biol, 2019, 25(6):1277-1285.DOI: 10.19675/j.cnki.1006-687x.2019.02014.
[33]	BRANT A N, CHEN H Y. Patterns and mechanisms of nutrient resorption in plants[J]. Critical Reviews in Plant Sciences, 2015, 34(5): 471-486. DOI:10.1080/07352689.2015.1078611.
[34]	OREN-SHAMIR M, LEVI-NISSIM A. Temperature effects on the leaf pigmentation of Cotinus coggygria‘Royal Purple’[J]. J Hortic Sci, 1997, 72(3):425-432.DOI: 10.1080/14620316.1997.11515530.
[35]	王峰, 王秀杰, 赵胜男, 等. 光对园艺植物花青素生物合成的调控作用[J]. 中国农业科学, 2020, 53(23):4904-4917.
	WANG F, WANG X J, ZHAO S N, et al. Light regulation of anthocyanin biosynthesis in horticultural crops[J]. Sci Agric Sin, 2020, 53(23):4904-4917.
[36]	谢智华, 姜卫兵, 韩键, 等. 早熟桃夏季红叶现象的生理机制研究[J]. 园艺学报, 2012, 39(7):1271-1277.
	XIE Z H, JIANG W B, HAN J, et al. Studies on physiological mechanism of red leaf phenomena of early maturing peach in summer[J]. Acta Hortic Sin, 2012, 39(7):1271-1277.DOI: 10.16420/j.issn.0513-353x.2012.07.011.
[37]	冯立娟, 苑兆和, 尹燕雷, 等. 槭属2品种叶变色期花青苷含量与相关酶活性的变化[J]. 林业科学, 2009, 45(8):56-60.
	FENG L J, YUAN Z H, YIN Y L, et al. Anthocyanin content and the relevant enzymes activities during leaf color changing of two Acer species[J]. Sci Silvae Sin, 2009, 45(8):56-60.DOI: 10.3321/j.issn:1001-7488.2009.08.010.
[38]	郑恬静, 王克凤, 桑瀚旭, 等. 两种海棠秋季叶色变化的生理机制研究[J]. 湖北农业科学, 2017, 56(15):2908-2912.
	ZHENG T J, WANG K F, SANG H X, et al. Study on physiological mechanism of leaf color change in autumn for two species of Malus baccata[J]. Hubei Agric Sci, 2017, 56(15):2908-2912.DOI: 10.14088/j.cnki.issn0439-8114.2017.15.028.
[39]	侯泽豪, 王书平, 魏淑东, 等. 植物花青素生物合成与调控的研究进展[J]. 广西植物, 2017, 37(12):1603-1613.
	HOU Z H, WANG S P, WEI S D, et al. Anthocyanin biosynthesis and regulation in plants[J]. Guihaia, 2017, 37(12):1603-1613.DOI: 10.11931/guihaia.gxzw201705002.
[40]	MURRAY J R, HACKETT W P. Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L[J]. Plant Physiol, 1991, 97(1):343-351.DOI: 10.1104/pp.97.1.343.
[41]	王惠聪, 黄旭明, 胡桂兵, 等. 荔枝果皮花青苷合成与相关酶的关系研究[J]. 中国农业科学, 2004, 37(12):2028-2032.
	WANG H C, HUANG X M, HU G B, et al. Studies on the relationship between anthocyanin biosynthesis and related enzymes in Litchi pericarp[J]. Sci Agric Sin, 2004, 37(12):2028-2032.DOI: 10.3321/j.issn:0578-1752.2004.12.043.
[42]	刘恺媛, 王茂良, 辛海波, 等. 植物花青素合成与调控研究进展[J]. 中国农学通报, 2021, 37(14):41-51.
	LIU K Y, WANG M L, XIN H B, et al. Anthocyanin biosynthesis and regulate mechanisms in plants:a review[J]. Chin Agric Sci Bull, 2021, 37(14):41-51.
[43]	ROSATI C, CADIC A, DURON M, et al. Molecular characterization of the anthocyanidin synthase gene in Forsythia × intermedia reveals organ-specific expression during flower development[J]. Plant Sci, 1999, 149(1):73-79.DOI: 10.1016/S0168-9452(99)00146-6.
[44]	万东璞, 于卓, 吴燕民, 等. 花青素代谢调控植物彩叶研究进展[J]. 中国农业科技导报, 2020, 22(2):30-38.
	WAN D P, YU Z, WU Y M, et al. Regulation of anthocyanin metabolism on colored leaves of plants[J]. J Agric Sci Technol, 2020, 22(2):30-38.DOI: 10.13304/j.nykjdb.2018.0233.
[45]	ZHAO Z C, HU G B, HU F C, et al. The UDP glucose:Flavonoid-3-O-glucosyltransferase (UFGT) gene regulates anthocyanin biosynthesis in Litchi (Litchi chinesis Sonn.) during fruit coloration[J]. Mol Biol Rep, 2012, 39(6):6409-6415.DOI: 10.1007/s11033-011-1303-3.
[46]	WU X X, GONG Q H, NI X P, et al. UFGT:the key enzyme associated with the petals variegation in Japanese apricot[J]. Front Plant Sci, 2017, 8:108.DOI: 10.3389/fpls.2017.00108.

指标 index	叶绿素 Chl	类胡萝卜素 Car	花青素 Ahc
L^*	0.656	0.060	-0.429
a^*	-0.959^**	-0.749	0.875^**
b^*	0.656	0.143	-0.461
类胡萝卜素/叶绿素Car/Chl	-0.917^**	-0.561	0.930^**
花青素/叶绿素Ahc/Chl	-0.748	-0.759^*	0.991^**
可溶性糖含量Ss content	-0.963^**	-0.678	0.926^**
可溶性蛋白含量Sp content	-0.904^**	-0.479	0.648
游离氨基酸含量Ac content	-0.766^*	-0.915^**	0.934^**
全氮含量total N content	0.967^**	0.668	-0.869^*
全磷含量total P content	0.885^**	0.522	-0.921^**
苯丙氨酸解氨酶PAL	-0.835^*	-0.688	0.977^**
二氢黄酮醇-4-还原酶DFR	-0.968^**	-0.479	0.797^*
花青素合成酶ANS	-0.975^**	-0.627	0.904^**
类黄酮3-O-糖基转移酶UFGT	-0.052	-0.142	0.509

黄连木变色期叶片色素变化规律及呈色机理

Changes in pigment and coloration mechanism of leaves during the discoloration period of Pistacia chinensis

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 46

相关文章 7

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	崔祺, 吴昀, 李东泽, 吴凡, 韩蕊莲, 黄均华, 胡绍庆. 彩叶桂叶片发育过程中叶色表型与色素成分变化[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 79-86.
[2]	夏晓雨, 王凤娟, 符群, 张娜, 郭庆启. 蓝莓果汁饮料原花青素的热稳定性及降解动力学模型[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 89-95.
[3]	宋协海,郭欢欢,刘勇,贺国鑫,薛敦孟,李成. 黄连木容器苗生长对缓释肥的响应[J]. 南京林业大学学报（自然科学版）, 2018, 42(03): 117-122.
[4]	母洪娜,孙陶泽,杨秀莲,王良桂. 两个桂花品种花色色素相关基因的差异表达[J]. 南京林业大学学报（自然科学版）, 2015, 39(03): 183-186.
[5]	潘彪,翟胜丞,黄建秋,林留根. 连云港藤花落史前古城遗址出土木材的树种鉴定[J]. 南京林业大学学报（自然科学版）, 2010, 34(05): 75-78.
[6]	张大生1,崔丽洁2,王景明1,吴少华2*. 红巴梨f3h基因的克隆及植物表达载体的构建[J]. 南京林业大学学报（自然科学版）, 2005, 29(02): 65-68.
[7]	马自超;莱依符. 蓝锭果红色素的分离与鉴定[J]. 南京林业大学学报（自然科学版）, 1987, 11(04): 67-71.